Recombinant Human Meprin A subunit alpha (MEP1A), partial

Code CSB-YP620984HU
MSDS
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Source Yeast
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Code CSB-EP620984HU
MSDS
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Source E.coli
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Code CSB-EP620984HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP620984HU
MSDS
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Source Baculovirus
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Code CSB-MP620984HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MEP1A
Uniprot No.
Alternative Names
Endopeptidase 2; Endopeptidase-2; Mep1a; MEP1A_HUMAN; Meprin A alpha (PABA peptide hydrolase) ; Meprin A alpha subunit precursor; Meprin A subunit alpha; N benzoyl L tyrosyl P amino benzoic acid hydrolase alpha subunit ; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha; PPHA
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Gene References into Functions
  1. Mep1A is overexpressed in most hepatocellular carcinomas and induces tumor cell migration and invasion. Mep1A expression is regulated by Reptin, and Mep1A mediates Reptin-induced migration. PMID: 27999200
  2. In this review we report on recent findings that summarize the complex molecular regulation of meprins, particular folding, activation and shedding. Dysregulation of meprin alpha and meprin beta is often associated with pathological conditions such as neurodegeneration, inflammatory bowel disease and fibrosis PMID: 28502593
  3. Results showed that MEP1A expression was elevated in colorectal cancer (CRC) tissues at both the mRNA and protein levels, and provided evidence for its functionally crucial role in CRC carcinogenesis as tumor proliferation and invasion. PMID: 27378469
  4. Variants rs17468190 (G/T) in the MEP1A gene are associated with glucose and insulin metabolism in overweight/obese central European women with polycystic ovary syndrome. PMID: 24388959
  5. Meprin metalloproteases A and B inactivate interleukin 6 PMID: 24474695
  6. Proteolytically active meprinalpha leads to an increase in EGFR and ERK1/2 phosphorylation and subsequently enhances cell proliferation and migration PMID: 22923609
  7. The present results indicate that a TNF-alpha-mediated down-regulation of CDX2 can be related to suppressed expression of MEP1A during intestinal inflammation. PMID: 22326557
  8. Meprin-alpha activity is regulated differently in primary tumors and metastases of colorectal cancer. By virtue of its pro-migratory and pro-angiogenic activity, meprin-alpha may promote tumor progression in colorectal cancer. PMID: 22096485
  9. Cystatin C was an inhibitor for human meprin alpha(K(i) = 8.5 x 10(-6) M) but, interestingly, not for meprin beta. PMID: 20806899
  10. meprins could be important players in several remodeling processes involving collagen fiber deposition PMID: 20631730
  11. activation in colorectal cancer triggered by the plasminogen-activating system PMID: 12189145
  12. The combination of these polymorphisms could not be found in any control individuals, suggesting that they might be involved in genetic predisposition to migraine in this family. PMID: 16378686
  13. Mephrin beta induced a dramatic change in cell morphology and reduced the cell number, indicating a function in terminal differentiation, whereas meprin alpha did not affect cell viability, and may play a role in basal keratinocyte proliferation. PMID: 17195012
  14. MEP1A is a UC susceptibility gene and indicate that decreased meprin-alpha expression is associated with intestinal inflammation in IBD patients and in a mouse experimental model of IBD. PMID: 19262505

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Peptidase M12A family
Database Links

HGNC: 7015

OMIM: 600388

KEGG: hsa:4224

STRING: 9606.ENSP00000230588

UniGene: Hs.179704

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