Recombinant Human Mesoderm posterior protein 1 (MESP1)

Code CSB-YP871559HU
MSDS
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Source Yeast
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Code CSB-EP871559HU
MSDS
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Source E.coli
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Code CSB-EP871559HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871559HU
MSDS
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Source Baculovirus
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Code CSB-MP871559HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MESP1
Uniprot No.
Alternative Names
bHLHc5; Class C basic helix-loop-helix protein 5; Mesoderm posterior 1; Mesoderm posterior 1 homolog (mouse); Mesoderm posterior 1 homolog; Mesoderm posterior protein 1; MESP 1; Mesp1; MESP1_HUMAN; MGC10676; MGC159208; MGC159210; OTTMUSP00000030151
Species
Homo sapiens (Human)
Expression Region
1-268
Target Protein Sequence
MAQPLCPPLS ESWMLSAAWG PTRRPPPSDK DCGRSLVSSP DSWGSTPADS PVASPARPGT LRDPRAPSVG RRGARSSRLG SGQRQSASER EKLRMRTLAR ALHELRRFLP PSVAPAGQSL TKIETLRLAI RYIGHLSAVL GLSEESLQRR CRQRGDAGSP RGCPLCPDDC PAQMQTRTQA EGQGQGRGLG LVSAVRAGAS WGSPPACPGA RAAPEPRDPP ALFAEAACPE GQAMEPSPPS PLLPGDVLAL LETWMPLSPL EWLPEEPK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
Gene References into Functions
  1. The present study firstly provided experimental evidence supporting the concept that a MESP1 lossoffunction mutation may contribute to the development of double outlet right ventricle in humans, which presents a significant insight into the molecular pathogenesis of congenital heart disease. PMID: 28677747
  2. results suggest that pathologic variants in MESP1 may contribute to the development of congenital heart disease (CHD) and that additional protein partners and downstream targets could likewise contribute to the wide range of causes for CHD PMID: 26694203
  3. The MESP1-NKX2-5 hESC reporter line allows us to identify molecular cues crucial for specification and expansion of human cardiac mesoderm and early progenitors and their differentiation to specific cardiovascular derivatives. PMID: 25187301
  4. MESP1 SNPs are associated with congenital heart disease in patients and altered transcription in vitro. PMID: 24056064
  5. Transcription factors ETS2 and MESP1 transdifferentiate human dermal fibroblasts into cardiac progenitors. PMID: 22826236
  6. MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling PMID: 18297060

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Subcellular Location
Nucleus.
Database Links

HGNC: 29658

OMIM: 608689

KEGG: hsa:55897

STRING: 9606.ENSP00000300057

UniGene: Hs.447531

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