Recombinant Human Meteorin (METRN)

In Stock
Code CSB-EP892322HU
Size US$306
Order now
Image
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP892322HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) METRN.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP892322HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) METRN.
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
METRN
Uniprot No.
Research Area
Neuroscience
Alternative Names
C16orf23; c380A1.2; Meteorin; Meteorin precursor ; meteorin; glial cell differentiation regulator; Metrn; METRN_HUMAN; MGC2601
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
24-293aa
Target Protein Sequence
GYSEERCSWRGSGLTQEPGSVGQLALACAEGAVEWLYPAGALRLTLGGPDPRARPGIACLRPVRPFAGAQVFAERAGGALELLLAEGPGPAGGRCVRWGPRERRALFLQATPHQDISRRVAAFRFELREDGRPELPPQAHGLGVDGACRPCSDAELLLAACTSDFVIHGIIHGVTHDVELQESVITVVAARVLRQTPPLFQAGRSGDQGLTSIRTPLRCGVHPGPGTFLFMGWSRFGEARLGCAPRFQEFRRAYEAARAAHLHPCEVALH
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
36.5 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Expressing the recombinant Human METRN protein in e.coli cells involves inserting a DNA fragment encoding the Human METRN protein (24-293aa) into a plasmid vector and transferring it to the e.coli cells. Positive cells are screened, cultured, and induced to express the METRN protein. The protein carries a N-terminal 10xHis tag and C-terminal Myc tag. The cells are lysed to collect the recombinant Human METRN protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human METRN protein exceeds 85%.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

 Q&A
Q:

Regarding CSB-EP892322HU (all expression systems), I would like to know if in liquid form (Tris-based buffer, 50% glycerol) if this product is soluble in cell culture medium or is it lyophilic?
Please let me know if you have any additional questions.

A:
Thanks for your inquiry. Sorry, these are semi-customized proteins that we haven't expressed before. Regarding this protein, we provide the soluble expression for Yeast, Baculovirus and Mammalian cell expression systems,
as for the E.coli expression system, we will try our best to optimize the condition to ensure soluble expression, If it is expressed in inclusion bodies, it will be renatured, and eventually gunaratee we will provide soluble proteins.
So we can guarantee that all of the proteins will be provided in soluble form. As for the lyophilic, we have used the software (https://web.expasy.org/protparam/) to predict, the result is -0.134, it should be lyophilic.
Q:

We would like to know the instructions for solubilizing the protein? If you have this information.
Please let me know if you have any additional questions.

A:
Thanks for your inquiry. As for the the instructions for solubilizing the protein, we may try a series of conditions to optimize the expression, such as optimizing the codon, the vector, the culture temperature, the incubation time, the concentration of the inducer, etc., try to do the soluble expression but not 100% guarantee, if it is an inclusion body expression, it will be renatured and we can guarantee that we will eventually provide soluble protein.

Target Background

Function
Involved in both glial cell differentiation and axonal network formation during neurogenesis. Promotes astrocyte differentiation and transforms cerebellar astrocytes into radial glia. Also induces axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia.
Gene References into Functions
  1. The joint measurements of circulating METRN levels in the first trimester and systolic blood pressure and weight in the second trimester significantly increase the probabilities of predicting preeclampsia. PMID: 26121675
  2. Meteorin plays important roles in both glial cell differentiation and axonal network formation during neurogenesis. PMID: 15085178
Subcellular Location
Secreted.
Protein Families
Meteorin family
Database Links

HGNC: 14151

OMIM: 610998

KEGG: hsa:79006

STRING: 9606.ENSP00000455068

UniGene: Hs.533772

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*