Recombinant Human Microtubule-associated protein 9 (MAP9)

Code CSB-YP013447HU
MSDS
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Source Yeast
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Code CSB-EP013447HU
MSDS
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Source E.coli
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Code CSB-EP013447HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013447HU
MSDS
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Source Baculovirus
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Code CSB-MP013447HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAP9
Uniprot No.
Alternative Names
ASAP; Aster associated protein; Aster-associated protein; Map9; MAP9_HUMAN; microtubule associated protein 9; Microtubule-associated protein 9
Species
Homo sapiens (Human)
Expression Region
2-647
Target Protein Sequence
SDEVFSTTL AYTKSPKVTK RTTFQDELIR AITARSARQR SSEYSDDFDS DEIVSLGDFS DTSADENSVN KKMNDFHISD DEEKNPSKLL FLKTNKSNGN ITKDEPVCAI KNEEEMAPDG CEDIVVKSFS ESQNKDEEFE KDKIKMKPKP RILSIKSTSS AENNSLDTDD HFKPSPRPRS MLKKKSHMEE KDGLEDKETA LSEELELHSA PSSLPTPNGI QLEAEKKAFS ENLDPEDSCL TSLASSSLKQ ILGDSFSPGS EGNASGKDPN EEITENHNSL KSDENKENSF SADHVTTAVE KSKESQVTAD DLEEEKAKAE LIMDDDRTVD PLLSKSQSIL ISTSATASSK KTIEDRNIKN KKSTNNRASS ASARLMTSEF LKKSSSKRRT PSTTTSSHYL GTLKVLDQKP SQKQSIEPDR ADNIRAAVYQ EWLEKKNVYL HEMHRIKRIE SENLRIQNEQ KKAAKREEAL ASFEAWKAMK EKEAKKIAAK KRLEEKNKKK TEEENAARKG EALQAFEKWK EKKMEYLKEK NRKEREYERA KKQKEEETVA EKKKDNLTAV EKWNEKKEAF FKQKEKEKIN EKRKEELKRA EKKDKDKQAI NEYEKWLENK EKQERIERKQ KKRHSFLESE ALPPWSPPSR TVFAKVF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in organization of the bipolar mitotic spindle. Required for bipolar spindle assembly, mitosis progression and cytokinesis. May act by stabilizing interphase microtubules.
Gene References into Functions
  1. MAP9 downregulation is associated with colorectal malignancy. PMID: 24876664
  2. ASAP stabilizes p53 both by alleviating MDM2-mediated p53 ubiquitination and by enhancing its p300-dependent acetylation. PMID: 22672907
  3. Results highlight dual ASAP regulation by Plk1 and further confirm the importance of ASAP for spindle pole organization, bipolar spindle assembly, and mitosis. PMID: 20615875
  4. ASAP is a human microtubule-associated protein required for bipolar spindle assembly and cytokinesis. PMID: 16049101
  5. Study demonstrates that AurA-mediated phosphorylation of ASAP is essential to spindle formation and mitosis. PMID: 17925329

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Note=Localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis.
Database Links

HGNC: 26118

OMIM: 610070

KEGG: hsa:79884

STRING: 9606.ENSP00000310593

UniGene: Hs.61271

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