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Recombinant Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4), partial

Recombinant Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4), partial

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Code	CSB-EP013514HU
Abbreviation	Recombinant Human MAST4 protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

MAST4

Uniprot No.

O15021

Research Area

Signal Transduction

Alternative Names

MAST4; MAST4_HUMAN; Microtubule associated serine/threonine protein kinase member 4; Microtubule-associated serine/threonine-protein kinase 4

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1140-1231aa

Target Protein Sequence

QPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

17.4 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human MAST4 contains amino acids 1140-1231. The expected molecular weight for the MAST4 protein is calculated to be 17.4 kDa. This MAST4 recombinant protein is manufactured in e.coli. The MAST4 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant MAST4 protein during the following stages.

The human microtubule-associated serine/threonine-protein kinase 4 (MAST4) plays a role in the regulation of various cellular processes, particularly those related to the cytoskeleton and cell morphology. As a member of the serine/threonine kinase family, MAST4 likely participates in signal transduction pathways, influencing cell growth, division, and migration. Studying MAST4 contributes to the broader understanding of kinase-mediated cellular signaling pathways.

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Target Background

Gene References into Functions

A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
high expression level of MAST4 in most normal human tissues, with an exception of in testis, small intestine, colon and peripheral blood leukocyte. PMID: 17086981

Subcellular Location

Cytoplasm.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family

Tissue Specificity

Highly expressed in most normal human tissues, with an exception of in testis, small intestine, colon and peripheral blood leukocyte.

Database Links

HGNC: 19037

KEGG: hsa:375449

STRING: 9606.ENSP00000385727

UniGene: Hs.595458

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