Recombinant Human Mitogen-activated protein kinase kinase kinase 5(MAP3K5) ,partial

Code CSB-YP013427HU
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Source Yeast
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Code CSB-EP013427HU
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Source E.coli
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Code CSB-EP013427HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013427HU
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Source Baculovirus
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Code CSB-MP013427HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names MAP3K5
Uniprot No. Q99683
Alternative Names Apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; ASK1; M3K5 ; M3K5_HUMAN; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; Mitogen activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Gene References into Functions
  1. Advanced glycation end products significantly activated ASK1, MKK3, and MKK6, which led to activation of p38 MAPK, resulting in upregulated fibrotic response in human coronary smooth muscle cells. PMID: 30305582
  2. ASK1 transcriptional upregulation molecularly defines a metabolically-detrimental obese sub-phenotype. PMID: 28702328
  3. Knockdown of miR-20a enhanced sensitivity of colorectal cancer cells to cisplatin through the ROS/ASK1/JNK pathway. PMID: 29940575
  4. Findings offer insight into positive regulation of Akt signaling through P2Y12 phosphorylation as well as MAPK signaling in platelets by ASK1. PMID: 28753204
  5. Cold stress-induced ferroptosis involves the ASK1-p38 pathway. PMID: 28887319
  6. TRIM48 Promotes ASK1 Activation and Cell Death through Ubiquitination-Dependent Degradation of the ASK1-Negative Regulator PRMT1 PMID: 29186683
  7. These findings indicate that chaetocin arrests the cell cycle and induces apoptosis by regulating the reactive oxygen species-mediated ASK-1/JNK signaling pathways. PMID: 28849240
  8. Findings provide evidence that ASK-1 expression is regulated by SLC35F2 which exerts its oncogenic effect on papillary thyroid carcinoma progression through activation of TGFBR-1 and ASK-1. PMID: 29274137
  9. Co-administration of acetaminophen and 5'-AMP significantly ameliorated APAP-induced hepatotoxicity in mice. This was triggered by attenuating apoptosis signal-regulated kinase 1(ASK1) methylation and increasing ubiquitination-mediated ASK1 protein degradation. PMID: 28031524
  10. The anti-cancer mechanism for the AgNPs may be involved in activating the ASK1-JNK/p38-Caspase-3 pathway. PMID: 29381295
  11. TRAF1 functions as a positive regulator of insulin resistance, inflammation, and hepatic steatosis dependent on the activation of ASK1-P38/JNK axis. PMID: 26860405
  12. LRRK2-induced apoptosis was suppressed by ASK1 inhibition in neuronal stem cells derived from patients with Parkinson's disease (PD). These results clearly indicate that LRRK2 acts as an upstream kinase in the ASK1 pathway and plays an important role in the pathogenesis of PD PMID: 28888991
  13. Apoptosis signal-regulating kinase 1 (ASK1) expression was dramatically suppressed and correlated with hepatocyte nuclear factor 4alpha (HNF4alpha) levels in hepatocellular carcinoma (HCC) tissues. PMID: 27050273
  14. ASK1 phosphorylated and stabilized TLX, which led induction of HIF-1alpha, and its downstream VEGF-A in an Akt dependent manner. PMID: 27890558
  15. CD40 activation resulted in down-regulation of Thioredoxin (Trx)-1 to permit ASK1 activation and apoptosis. Although soluble receptor agonist alone could not induce death, combinatorial treatment incorporating soluble CD40 agonist and pharmacological inhibition of Trx-1 was functionally equivalent to the signal triggered by mCD40L PMID: 27869172
  16. These results suggest that the platelet Ask1 plays an important role in regulation of hemostasis and thrombosis. PMID: 28028021
  17. from the two catalytic cysteines of TRX1 the residue C32 is responsible for the high-affinity binding of TRX1 to the ASK1-TRX-binding domain in reducing conditions PMID: 27588831
  18. Shotgun mass spectrometry and manual validation identified 12 distinct ASK1 phosphosites. Targeted parallel reaction monitoring assays were used to track the phosphorylation dynamics of each confirmed site in response to treatment. PMID: 27989136
  19. phosphorus NMR and time-resolved tryptophan fluorescence measurements suggest that 14-3-3zeta interacts with the kinase domain of ASK1 in close proximity to its active site, thus indicating this interaction might block its accessibility and/or affect its conformation. PMID: 27514745
  20. ASK1 MAP kinase signaling cascade is an important regulator of chondrocyte terminal differentiation. PMID: 26405834
  21. Pretreatment by IRE1 agonist tunicamycin or JNK agonist anisomycin attenuated the effect of psoralen on osteoporotic osteoblasts. Psoralen inhibited apoptosis of osteoporotic osteoblasts by regulating IRE1-ASK1-JNK pathway PMID: 28349059
  22. Our results thus suggest that GSK-3beta is a key factor involved in ASK1 activation and reactive oxygen species-induced cell death. PMID: 27221474
  23. the data show that miRNA-mediated down-regulation of ASK1 protects mesenchymal stem cells during post-transplantation, leading to an increase in the efficacy of MSC-based cell therapy. PMID: 27775615
  24. Cross-talk between arginine methylation and serine phosphorylation in ASK1.PRMT5 is an ASK1-binding protein.PRMT5 mediates arginine methylation of ASK1. PMID: 26912789
  25. results suggest that baicalein-mediated ASK1/JNK activation regulates the mitochondria-dependent apoptosis pathway through the up-regulation of TAp63 and down-regulation of NF-kappaB and CD74/CD44 in B-cell malignancies PMID: 26694167
  26. curcumin and ABT-737 on HCC cells was investigated for the first time, to the best of our knowledge. It was found that curcumin markedly enhanced the antitumor effects of ABT-737 on HepG2 cells and activate ROS-ASK1-c-Jun N-terminal kinase pathway . . PMID: 26707143
  27. ASK1 signalling regulates brown and beige adipocyte function. PMID: 27045525
  28. These results implicate the TNF/TRAF2/ASK1/p38 kinase pathway in modulating risk of pulmonary complications. PMID: 26165383
  29. The present findings thus support our notion that ROR1 sustains lung adenocarcinoma survival, at least in part, through direct physical interaction with ASK1 PMID: 26661061
  30. Together, we suggest that 4SC-202 activates ASK1-dependent mitochondrial apoptosis pathway to potently inhibit human HCC cells. PMID: 26773495
  31. Data show that the MAPKKK6 ASK2, a modulator of MAPKKK5 ASK1 signaling, was essential for ASK1-dependent apoptosis, but not for inducing interferon-beta (IFNB) expression. PMID: 26243192
  32. The expression of ASK1 is correlated with the level of claudin-6 in cervical carcinoma cells and tissues. PMID: 26191261
  33. ASK1 stabilizes APOBEC3G and binds HIV-1 Vif, disrupting assemble of the Vif-ubiquitin ligase complex, thus restoring the antiviral activity of APOBEC3g. PMID: 25901786
  34. Data indicates that ASK1 expression is regulated by MiR-19a by targeting specific sites in the 3' untranslated region of its mRNA. PMID: 25982447
  35. Findings suggest that methyl isocyanate inhibits angiogenesis by inducing mitogen-activated protein kinase kinase kinase 5 ASK1-JNK-dependent endothelial cell death. PMID: 25068797
  36. TNF-alpha-induced ASK1-p38/JNK pathway is an important mediator of cytokine synthesis and enhanced expression of adhesion molecules in rheumatoid arthritis and is inhibited by thymoquinone. PMID: 26134265
  37. Cyclophilin A regulates JNK/p38-MAPK signaling through its physical interaction with ASK1. PMID: 26095851
  38. Knockdown of IRE1alpha by siRNA dramatically abrogated CXC195-induced activation of TRAF2, ASK and JNK, formation of an IRE1alpha-TRAF2-ASK1 complex and caspase- and mitochondrial-dependent apoptosis in T24 cells PMID: 25797626
  39. Because the phosphorylation site mutants of NR4A2 cannot rescue the cell death-promoting activity, ASK1-p38 pathway-dependent phosphorylation and subsequent cytoplasmic translocation of NR4A2 may be required for oxidative stress-induced cell death. PMID: 25752609
  40. Collectively, these data reveal that activation of the PI3K/Akt pathway limits JNK-mediated apoptosis by phosphorylating and inactivating ASK1 during human enterovirus 71 infection. PMID: 25116390
  41. Siah1 is a substrate of ASK1 for activation of the GAPDH-Siah1 oxidative stress signaling cascade. PMID: 25391652
  42. TNF-signaling dependence of ASK1-mediated apoptosis in melanoma cells. PMID: 24574456
  43. Data show that ASK1 is critical for IFN gamma-induced DAPK1 via ATF6 recruitment. PMID: 25135476
  44. apoptosis signal-regulating kinase 1 has a role in chondrosarcoma cell apoptosis along with endoplasmic reticulum stress due to FPipTB PMID: 21594902
  45. data suggest that degradation of ASK1 mediated by Roquin-2 is an evolutionarily conserved mechanism required for the appropriate regulation of stress responses, including pathogen resistance and cell death PMID: 24448648
  46. It is activated in response to various stresses, such as reactive oxygen species (ROS)and endoplasmic reticulum (ER) stress, and plays pivotal roles in a wide variety of cellular responses, including cell death, differentiation and inflammation. (review) PMID: 24912301
  47. Data indicate that the ASK1-FoxO3a-TRADD-caspase 8 pathway is present in neural tube defects (NTDs)-affected tissues. PMID: 23982205
  48. MAP3K5 R256C mutation revealed attenuation of MKK4 activation through increased binding of the inhibitory protein thioredoxin (TXN/TRX-1/Trx), resulting in increased proliferation and anchorage-independent growth of melanoma cells. PMID: 24008424
  49. Identification of the domain through which HIV-1 Nef interacts with ASK1 and inhibits its function. PMID: 23799149
  50. In gastric epithelial cells, H. pylori activates ASK1 in a reactive oxygen species - and cag pathogenicity island-dependent manner, and ASK1 regulates sustained JNK activation and apoptosis induced by H. pylori. PMID: 24082073

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Subcellular Location Cytoplasm. Endoplasmic reticulum. Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
Protein Families Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
Tissue Specificity Abundantly expressed in heart and pancreas.
Database Links

HGNC: 6857

OMIM: 602448

KEGG: hsa:4217

STRING: 9606.ENSP00000351908

UniGene: Hs.186486

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