MAP3K5 Research Reagents

Mitogen-activated protein kinase kinase kinase 5 is a protein in humans that is encoded by MAP3K5 gene. Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens.

The following MAP3K5 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

MAP3K5 Antibodies

MAP3K5 Antibodies for Homo sapiens (Human)

MAP3K5 Proteins

MAP3K5 Proteins for Mus musculus (Mouse)

MAP3K5 Proteins for Homo sapiens (Human)

MAP3K5 Background

The MAP3K5 gene encodes the mitogen-activated protein kinase kinase kinase 5, an enzyme also known as apoptosis signal-regulating kinase 1 (ASK1). Under nonstressed conditions, MAP3K5/ASK1 forms a high molecular mass complex, designated as the ASK1 signalosome [1]. In the signalosome, ASK1 is homo-oligomerized through the C-terminal coiled-coil domain (CCC). Following hydrogen peroxide stimulation, ASK1 undergoes autophosphorylation, leading to ASK1 activation [2][3]. MAP3K5/ASK1 is a member of the mitogen-activated protein kinase kinase kinase family, which activates c-Jun N-terminal kinase and p38 in response to a diverse array of stresses such as oxidative stress, endoplasmic reticulum stress, and calcium influx. These protein kinases are sequentially activated, such that the MAPK kinase kinase (MAPKKK) phosphorylates and activates the MAPK kinase (MAPKK), which in turn phosphorylates and activates the MAPK, eventually inducing proliferation, differentiation, migration, and apoptosis [4]. Besides, MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Furthermore, overexpression of ASK1 wild-type or a constitutively active mutant induced apoptosis in a fetal lung cell line [5][6]. ASK1 is involved in cancer, diabetes, rheumatoid arthritis, cardiovascular and neurodegenerative diseases [7][8].

[1] Noguchi T, Takeda K, et al. Recruitment of tumor necrosis factor receptor-associated factor family proteins to apoptosis signal-regulating kinase 1 signalosome is essential for oxidative stress-induced cell death [J]. J Biol Chem. 2005, 280: 37033-37040.
[2] Liu H, Nishitoh H, et al. Activation of apoptosis signal-regulating kinase 1 (ASK1) by tumor necrosis factor receptor-associated factor 2 requires prior dissociation of the ASK1 inhibitor thioredoxin [J]. Mol Cell Biol. 2000, 20: 2198-2208.
[3] Fujino G, Noguchi T, et al. Thioredoxin and TRAF family proteins regulate reactive oxygen species-dependent activation of ASK1 through reciprocal modulation of the N-terminal homophilic interaction of ASK1 [J]. Mol Cell Biol. 2007, 27: 8152-8163.
[4] Nagai H, Noguchi T, et al. Pathophysiological roles of ASK1-MAP kinase signaling pathways [J]. J Biochem Mol Biol. 2007 Jan 31;40(1):1-6.
[5] Johnson GL, Dohlman HG, et al. MAPK kinase kinases (MKKKs) as a target class for small-molecule inhibition to modulate signaling networks and gene expression [J]. Curr Opin Chem Biol. 2005, 9: 325-331.
[6] Ichijo H, Nishida E, et al. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways [J]. Science. 1997, 275: 90-94.
[7] Hattori K, Naguro I, et al. The roles of ASK family proteins in stress responses and diseases [J]. Cell Communication and Signaling. 2009, 7: 9.
[8] Nygaard G, Di Paolo JA, et al. Regulation and function of apoptosis signal-regulating kinase 1 in rheumatoid arthritis [J]. Biochemical Pharmacology. 2008, 151: 282–290.

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