Recombinant Human MutS protein homolog 5 (MSH5), partial

Code CSB-YP015031HU
MSDS
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Source Yeast
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Code CSB-EP015031HU
MSDS
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Source E.coli
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Code CSB-EP015031HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015031HU
MSDS
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Source Baculovirus
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Code CSB-MP015031HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MSH5
Uniprot No.
Alternative Names
DKFZp434C1615; G7; hMSH5; MGC2939; MSH 5; MSH5; MSH5_HUMAN; mutS (E. coli) homolog 5 ; mutS homolog 5 ; mutS homolog 5 (E. coli); MutS protein homolog 5; MutSH5; NG23; OTTHUMP00000029187; OTTHUMP00000029188
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in DNA mismatch repair and meiotic recombination processes. Facilitates crossovers between homologs during meiosis.
Gene References into Functions
  1. From sanger sequencing of MSH5 in 200 sporadic POI patients, we identified three heterozygous mutations . Considering the heterozygous p.D487Y carrier in the POI pedigree was fertile, the causality of the three heterozygous mutations in POI need more evidence PMID: 28175301
  2. our data point to the existence of a functional interplay between hMSH5 and FANCJ in double-strand break repair induced by replication stress. PMID: 26055704
  3. the roles of hMSH5 variants in the processes of DNA damage response and repair PMID: 24023853
  4. Authors show that MSH5 (MutSHomolog 5) is localized into the mitochondria of germ and somatic cells. PMID: 22917773
  5. Study has suggested a role for hMSH5 in the processing of cisplatin-induced DSBs, and silencing of hMSH5 may provide a new means to improve the therapeutic efficacy of cisplatin. PMID: 22401567
  6. The presence of the MSH5 85F allele marks the subgroup of DRB1*0102 haplotypes carrying susceptibility for selective IgA deficiency. MSH5 polymorphisms per se are not predisposing factors. PMID: 20542071
  7. hMSH5 possesses a CRM1-dependent nuclear export signal and a nuclear localization signal that participates to its nuclear targeting. PMID: 20185565
  8. There is an association of polymorphism C85T in MSH5 or C2531T in MLH3 with male infertility, specifically azoospermia or severe oligozoospermia, and interaction between these MSH5 and MLH3 polymorphisms increased the risk of developing male infertility PMID: 19808033
  9. A review of the properties and functional roles of MSH5. PMID: 17127347
  10. These findings suggest that Msh4/5 heterodimers contribute to class switch recombination. PMID: 17409188
  11. Data show that MSH5 mutations may be one explanation for premature ovarian failure. PMID: 18166824
  12. Biochemical identification of the proteins with which it interacted showed that SMCY formed a distinct complex with MSH5, a critical meiosis-regulatory protein in the human testicular germ cell line, NEC8. PMID: 18459961
  13. this study implicates a role for hMSH5 in DNA damage response involving c-Abl and p73, and suggests that mutations impairing this process could significantly affect normal cellular responses to anti-cancer treatments. PMID: 19442657
  14. occurrence of an unusual TG 3' splice site in intron 6 PMID: 17672918

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Involvement in disease
Premature ovarian failure 13 (POF13)
Protein Families
DNA mismatch repair MutS family
Tissue Specificity
Widely expressed, with high levels in testis and ovary, including granulosa cells. Also expressed in fetal ovary and adrenal gland.
Database Links

HGNC: 7328

OMIM: 603382

KEGG: hsa:4439

STRING: 9606.ENSP00000364855

UniGene: Hs.647011

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