Recombinant Human Myosin regulatory light chain 12A (MYL12A) (Active)

MYL12A is a non-muscle myosin regulatory light chain that modulates actomyosin contractility, making it a key node in studies of cell migration, cytokinesis, and cancer-associated cytoskeletal remodeling. This full-length recombinant human MYL12A (aa 1–171), expressed in *E. coli* with a C-terminal 6×His tag positioned away from the N-terminal EF-hand domains, preserves the native conformation required for productive protein-protein interactions. Functional ELISA confirms binding activity against an anti-MYL9 recombinant antibody with an EC₅₀ of 5.325–6.456 ng/mL, providing a suitable basis for antibody development, epitope mapping, and use as a positive control in western blot or immunofluorescence workflows. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for structural studies such as crystallography or cryo-EM, as well as cytoskeletal dynamics and polymerization assays investigating actomyosin complex assembly.