Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

DDAH2

Uniprot No.

O95865

Research Area

Neuroscience

Alternative Names

(DDAH-2)(Dimethylarginine dimethylaminohydrolase 2)(DDAHII)(Dimethylargininase-2)(Protein G6a)(S-phase protein)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-285aa

Target Protein Sequence

MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEIGDENATLDGTDVLFTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGPSHLRGLCGMGGPRTVVAGSSDAAQKAVRAMAVLTDHPYASLTLPDDAAADCLFLRPGLPGVPPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSELEKAGAGLSSLCLVLSTRPHS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

61.2 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The process of expressing the recombinant Human DDAH2 protein includes transfecting e.coli cells with a DNA expression vector carrying the gene for the desired protein (1-285aa). Subsequently, the cells are cultured to induce the expression of the intended protein. The protein is equipped with a N-terminal 6xHis-GST tag. The recombinant Human DDAH2 protein is then collected and purified from the cell lysate through affinity purification, exhibiting a purity exceeding 85%, as verified by SDS-PAGE.

Target Background

Function

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.

Gene References into Functions

genetic variations in the DDAH2 gene may influence the ADMA concentration and erythropoietin resistance in MHD patients PMID: 28590543
The main finding from this study was that it demonstrated that the C-allele of rs3087894 in DDAH1 is a risk factor for hypertension in the Kazakh group but a protective factor in the Uygur group. In addition, we did not find any genotype of DDAH1 and DDAH2 associated with hypertension in the Han group. PMID: 26786611
Exogenous human DDAH2 gene promotes differentiation of rabbit bone marrow-derived endothelial progenitor cells into mature endothelial cells. PMID: 28150318
expression of DDAH2 is associated with invasiveness of lung adenocarcinoma via tumor angiogenesis PMID: 26515557
Inhibiting the expression of DDAH1, but not DDAH2, resulted in a significant increase in the sensitivity of the EVT cell line SGHPL-4 to tumour necrosis factor related apoptosis inducing ligand (TRAIL) induced apoptosis PMID: 26082478
increased ADMA levels in rheumatoid arthritis do not appear to relate to DDAH genetic polymorphisms PMID: 25194333
The percentage of senescent endothelial progenitor cells increased while the expression of DDAH2 decreased concomitantly with an increase in the plasma levels of asymmetric dimethylarginine in patients with type 2 diabetes mellitus. PMID: 25701782
DDAH-1 is a specific molecular target for portal pressure reduction, through actions on ADMA-mediated regulation of eNOS activity. PMID: 25152204
Our results suggest that the rs805304 C allele of the DDAH gene was associated with decreased risk of myocardial infarction and decreased risk of obesity. PMID: 25236572
Homocysteine disrupts EPCs function via inducing the hypermethylation of DDAH2 promoter, suggesting a key role of epigenetic mechanism in the progression of atherosclerosis PMID: 24934151
-476 to -469 of the DDAH2 promoter was a NF-kB responsive element and is important for the transactivation of DDAH2. PMID: 24928011
The rs9267551 functional variant of the DDAH2 gene is associated with chronic kidney disease with carriers of the C allele having a lower risk of renal dysfunction independently from several confounders PMID: 24125425
Our approaches revealed signature candidates of differentially hypermethylated genes of DDAH2 and DUSP1 which can be further developed as potential biomarkers for OSCC as diagnostic, prognostic and therapeutic targets in the future. PMID: 24155659
Enhancing pulmonary DDAH II activity attenuates LPS-mediated lung leak in acute lung injury. PMID: 24134589
There is a significant difference in distribution of DDAH2 gene polymorphism among hemodialysis patients compared to healthy individuals. PMID: 23770786
DDAH2-1151 A/C polymorphism is associated with Chronic renal impairment in type 2 diabetes. PMID: 23129820
Glucose-stimulated insulin secretion is increased in Ddah2-transgenic pancreatic islets by 33%, compared to its levels in wild-type mice. PMID: 23430976
Suppression of DDAH2 expression is a culprit for homocysteine-induced impairments of DDAH/ADMA/NOS/NO pathway in endothelial cells. PMID: 23171931
Found that SNP rs2272592 in DDAH2 is associated with type 2 diabetes but SNP rs805304 in DDAH2 is not. DDAH2 SNP rs2272592 AG+GG genotypes are associated with genetic susceptibility to type 2 diabetes in Korean population. PMID: 22579530
No association was observed between the DDAH2 polymorphisms at rs805305 and rs2272592 and coronary heart disease. PMID: 22923027
A functional polymorphism of the DDAH2 gene may confer increased risk for type 2 diabetes by affecting insulin sensitivity PMID: 22558392
The -449G single nucleotide polymorphism within the DDAH2 gene was associated with both decreased plasma asymmetric dimethylarginine and an increased likelihood of presenting with "cold" shock in pediatric sepsis. PMID: 22428028
DDAH-2 could play an important role in IL-1beta-induced NO production and in osteoarthritis pathogenesis. PMID: 21898353
Low expression of DDAH-2 in placenta and increased serum asymmetric dimethylarginine level might confer susceptibility to preeclampsia. PMID: 19570459
a possible association between A allele / AA genotype for DDAH2 SNP1 (-1151 C/A, rs805304) and G allele / GG genotype for SNP2 (-449 C/G, rs805305) with CVD in male 35-50 year-old Egyptian patients was observed. PMID: 21677405
DDAH1 and DDAH2 polymorphisms are strongly and additively associated with serum ADMA concentrations in individuals with type 2 diabetes PMID: 20209122
Single nucleotide polymorphisms in the DDAH2 gene are associated with blood pressure levels, prevalence of hypertension, and left ventricular mass and function in the general population. PMID: 19666123
In cultured endothelial cells, heterologously expressed human DDAH II was S-nitrosylated after cytokine induced expression of the inducible NOS isoforms. PMID: 12370443
DDAH2 expression in endothelial cells is altered by genetic variation in a basal promoter element PMID: 14550280
messenger RNA and protein were demonstrated in first trimester placental tissue, primary extravillous trophoblasts and extravillous trophoblast-derived cell lines. PMID: 16920729
Allelic variation for a polymorphism in the DDAH II gene may influence asymmetrical dimethyl arginine concentrations, hence the severity of organ failure in septic shock patients. PMID: 17002794
The purpose of this study was to investigate whether there is any association between preeclampsia and eNOS, DDAH, and VEGF gene polymorphisms.Polymorphisms in eNOS, DDAH, and VEGF gene do not seem to be risk factors for preeclampsia. PMID: 18251679
Lysophosphatidylcholine (LPC) impairs DDAH/ADMA/NOS/NO pathway, and DDAH2 gene transfer could improve the LPC-elicited impairments in endothelial cells. PMID: 18342305
results suggest that the DDAH2 common variant may play a protective role in the development of Intracerebral Hemorrhage, implicating that the DDAH2/ADMA pathway may act as a critical regulator of cerebral small-vessel disorders PMID: 19250061
DDAH2 mRNA expression is inversely associated with some cardiovascular risk-related features. PMID: 19822957

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Subcellular Location

Cytoplasm. Mitochondrion. Note=Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.

Protein Families

DDAH family

Tissue Specificity

Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.

Database Links

HGNC: 2716

OMIM: 604744

KEGG: hsa:23564

STRING: 9606.ENSP00000364943

UniGene: Hs.247362

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Code	CSB-EP006580HU
Abbreviation	Recombinant Human DDAH2 protein
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human N (G),N (G)-dimethylarginine dimethylaminohydrolase 2 (DDAH2)

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