Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ME3

Uniprot No.

Q16798

Research Area

others

Alternative Names

ME3NADP-dependent malic enzyme; mitochondrial; NADP-ME; EC 1.1.1.40; Malic enzyme 3

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

24-590aa

Target Protein Sequence

RWTPTAPAQGCHSKPGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

67.2 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Dive into the world of enzymatic research with our premium Recombinant Human ME3 protein. This NADP-dependent malic enzyme, mitochondrial (NADP-ME; Malic enzyme 3) is specifically engineered in an E. coli expression system, providing a reliable and efficient product for your research needs. The partial protein (24-590aa) ensures outstanding performance in various experimental setups.

Featuring an N-terminal 6xHis-tag for easy protein purification and detection, our Recombinant Human ME3 protein boasts a purity greater than 85% as determined by SDS-PAGE. The protein is available in both liquid and lyophilized powder forms, providing versatility and flexibility for your research applications. This premium product supports your investigations into the intricate world of enzymatic processes.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

According to the expression system above, this is the partial protein (24-590aa). I would like the full-length sequence from 1-590aa. Do you have this protein? Or is the sequence 1-23aa just the signal sequence and the protein above is the mature full-length form (so not partial)? Can you please clarify. If you have the full-length protein 1-590aa, can you please provide pricing for all expression systems, tag info, sequence, etc.

Thanks for your inquiry. The 1-23aa is mitochondrial transit peiptide, and the 24-590aa is the full length of the mature protein.

We are looking for functionally active recombinant human ME3 for an enzyme assay. Kindly confirm suitability of your product for the same. Also share specific activity of the protein if you have tested.

Thanks for your inquiry.
We haven't tested the activity of this protein yet so we can't provide activity data currently.
We could provide you 10ug of Free Sample, in the condition that you share their result with us after activity test.
You don't need to provide us the raw data, just tell us the activity testing method (substrate and ligand used), result (Active/ Inactive), and published literature link.

Target Background

Gene References into Functions

ATP inhibition is proposed to be determined by the electrostatic potential involving the positive charge on the side chain of Lys346 PMID: 18959763
Studies demonstrate that the Lys57 residue plays dual functional roles in the structural integrity of the allosteric site and in the subunit-subunit interaction at the dimer interface of m-NAD(P)-ME. PMID: 19236308
human c-NADP-ME exists mainly as a tetramer, whereas human m-NAD(P)-ME exists as a mixture of dimers and tetramers PMID: 19416979

Subcellular Location

Mitochondrion matrix.

Protein Families

Malic enzymes family

Tissue Specificity

Expressed predominantly in organs with a low-division rate.

Database Links

HGNC: 6985

OMIM: 604626

KEGG: hsa:10873

STRING: 9606.ENSP00000352657

UniGene: Hs.199743

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