Recombinant Human Na (+)/H (+) exchange regulatory cofactor NHE-RF3 (PDZK1)

Code CSB-EP722565HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Greater than 90% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Signal Transduction
Alternative Names
1700023D20Rik; 2610507N21Rik; 4921513F16Rik; AI267131; AI314638; AL022680; C terminal linking and modulating protein; CAP70; CFTR associated protein of 70 kDa ; CFTR associated protein; 70-KD; CFTR-associated protein of 70 kDa; CLAMP; D3Ertd537e; Dietary Pi-regulated RNA-1; Diphor-1; mPDZK1; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; Na/Pi cotransporter C-terminal-associated protein; NaPi Cap1; NaPi-Cap1; NaPiCap1; NHERF 3; NHERF-3; NHERF3; NHRF3_HUMAN; OTTHUMP00000015572 ; PDZ domain containing 1; PDZ domain containing protein 1; PDZ domain-containing protein 1; PDZD1; PDZK1; Sodium hydrogen exchanger regulatory factor 3; Sodium-hydrogen exchanger regulatory factor 3
Homo sapiens (Human)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma membrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
Gene References into Functions
  1. PDZK1 directly interacts with OATP2B1 resulting in a change of the amount of transporter in the membrane, and thereby in an increased transport function. PMID: 29752999
  2. These findings have provided first lines of evidences that PDZK1 expression is negatively correlated with SHP-1 activation and poor clinical outcomes in clear cell renal cell carcinoma (ccRCC) . PDZK1 was identified as a novel tumor suppressor in ccRCC by negating SHP-1 activity PMID: 28692056
  3. HNF1alpha, which has previously been described as a modulator of several transporters of the renal transportosome, is also a key determinant of PDZK1 transcription. PMID: 28724612
  4. a PDZK1 single nucleotide polymorphism rs12129861 was found to be significantly associated with gout susceptibility (meta-analysis) PMID: 27720648
  5. PDZK1 was defined as an independent prognostic factor for both OS and DFS. These findings indicated that low level of PDZK1 could predict poor clinical outcome in patients with ccRCC. PMID: 27993630
  6. Inflammation-induced loss of PDZK1 expression may contribute to the NHE3 dysfunction observed in the inflamed intestine. PMID: 25271043
  7. PARP regulates estradiol-mediated cell growth by controlling the ER/IGF-1R/PDZK1 axis. PMID: 26183824
  8. PDZKA1 adaptor protein gene of urate transporters, is not associated with gout. PMID: 25362723
  9. PDZK1, negatively regulates 5-HT2AR endocytosis and has no effect upon 5-HT2AR-mediated ERK1/2 phosphorylation. PMID: 25562428
  10. Described is a structure of D-AKAP2 in complex with two interacting partners and the exact mechanism by which a segment that on its own is disordered presents an alpha-helix to PKA and a beta-strand to PDZK1. PMID: 25348485
  11. PDZK1 and NHERF1 regulate the transport function of OATP1A2 by modulating protein internalization via a clathrin-dependent pathway and by enhancing protein stability. PMID: 24728453
  12. Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37 PMID: 24869908
  13. IRF3 activation by innate antiviral signaling represses TGF-beta-induced growth inhibition, gene regulation and epithelial-mesenchymal transition, and the generation of Treg effector lymphocytes from naive CD4(+) lymphocytes. PMID: 25526531
  14. NHERF3 is a key regulator of organic transport in the kidney, particularly MRP4-mediated clearance of drug molecules. PMID: 24436471
  15. These results clarify the relationship between ER-alpha and PDZK1, propose a direct relationship between PDZK1 and IGF1R, and identify a novel oncogenic activity for PDZK1 in breast cancer. PMID: 23821363
  16. Antibody screening revealed 3 candidate prognostic markers in breast cancer: the Anillin (ANLN); PDZ-Binding Kinase (PBK); and, PDZ-Domain Containing 1 (PDZK1). PMID: 23547718
  17. Upregulation of PDZK1 could have an important role in the development of melasma in connection with estrogen through NHE, CFTR, and SLC26A3. PMID: 22696060
  18. PDZ domain-containing 1 (PDZK1) protein regulates phospholipase C-beta3 (PLC-beta3)-specific activation of somatostatin by forming a ternary complex with PLC-beta3 and somatostatin receptors. PMID: 22528496
  19. Human prostacyclin receptor interacts with the PDZ adapter protein PDZK1; this interaction plays important role in endothelial cell migration and angiogenesis. PMID: 21653824
  20. Although PDZK1 binding is required for optimal cell surface expression of oatp1a1, phosphorylation provides a mechanism for fast regulation of the distribution of oatp1a1 between the cell surface and intracellular vesicular pools. PMID: 21183661
  21. The SR-BI partner PDZK1 facilitates hepatitis C virus entry. PMID: 20949066
  22. Data show that PDZK1/EBP50/ezrin form a regulated ternary complex in vitro and in vivo. PMID: 20237154
  23. Overexpression of PDZK1 is associated with a drug-resistance phenotype in multiple myeloma. PMID: 15215163
  24. PDZK1 plays a role in regulating the functional activity of URAT1-mediated urate transport in the apical membrane of renal proximal tubules. PMID: 15304510
  25. We have examined the ability of Bcr to interact with other epithelial PDZ proteins and found specific binding to both the apical PDZK1 protein and the Golgi-localized Mint3 PMID: 15494376
  26. Double transfection of OCTN2 with PDZK1 intestinal and kidney-enriched PDZ protein))stimulated the uptake by OCTN2 of its endogenous substrate carnitine. PMID: 15523054
  27. oligomerization of Oatp1a1 with PDZK1 is critical for its proper subcellular localization and function PMID: 15994332
  28. intracellular sorting of the somatostatin receptor subtype 5 is regulated by interactions with PDZ domain proteins PIST/GOPC and PDZK1 PMID: 16012170
  29. PEPT2-PDZK1 interaction thus plays a physiologically important role in both oligopeptide handling as well as peptide-like drug transport in the human kidney PMID: 16738539
  30. The interaction of PDZ proteins with hOAT4 may be cell-specific. In placenta, a different set of interacting proteins from PDZK1 and NHERF1 may be required to modulate hOAT4 activity. PMID: 17602283
  31. Pdzk1 plays a specific role in stabilizing Muc17 in the apical membrane of small intestinal enterocytes. PMID: 17990980
  32. Established a mouse model to study human reverse cholesterol transport by expressing CLA-1, human PDZK1, and human apoA-I gene. PMID: 18403724
  33. Endogenous PPARalpha regulates PDZK1 expression. PMID: 18955051
  34. While the presence of forskolin results in an increase in OCTN2 protein expression, the increase in uptake capacity may be compensated by the decreased expression of PDZK1, NHERF1 or NHERF2. PMID: 19091402
  35. The tail of PDZK1 interacts with the PDZ domains of EBP50, and this interaction is negatively regulated by the intramolecular association of the tail of PDZK1 with its first PDZ domain. PMID: 19173579
  36. findings suggest that PDZ domain containing 1 (PDZK1)_i33968C > T genetic variants may be associated with a higher risk of exhibiting metabolic syndrome PMID: 19321583
  37. In HEK cells, which express little PDZK1, additional transfection of PDZK1 was required for UTP to inhibit DRA. PMID: 19447883
  38. NHERF3 colocalizes and directly binds NHE3 at the plasma membrane under basal conditions. PMID: 19535329

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Subcellular Location
Membrane; Peripheral membrane protein. Cell membrane.
Protein Families
NHER family
Tissue Specificity
Expression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule), pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carci
Database Links

HGNC: 8821

OMIM: 603831

KEGG: hsa:5174

STRING: 9606.ENSP00000342143

UniGene: Hs.444751

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