Recombinant Human Na (+)/H (+) exchange regulatory cofactor NHE-RF1 (NHERF1)

1. DNMT1 contributes to promoter hypermethylation and epigenetic NHERF1 silencing in colon cancer. PMID: 29901158
2. found that NHERF1 was associated with trophoblast differentiation and motility; stepwise experimental platform to explore new functions of ambiguously denoted candidate proteins PMID: 28960081
3. High expression of NHERF-1 is associated with triple-negative breast cancer. PMID: 28535016
4. Taken together, these findings suggest that the high dynamics of cytosolic NHERF1 provide cancer cells with a means of controlling chemotactic migration. PMID: 28684865
5. The up-regulation of NHERF1 induced by the exposure to hypoxia in colon cancer cells depends on the activation of VEGFR2 signaling. PMID: 27999191
6. -catenin was predominantly associated with Na+/H+ exchanger regulating factor 1 (NHERF1) in a dynamic context and an increase of its oncogenic function through RAS-association domain family 1, isoform A (RASSF1A) inactivation in liver metastases. PMID: 27765918
7. Na+/H+ exchanger regulatory factor (NHERF1) expression level positively associates with G protein-coupled estrogen receptor (GPER) activation in ER-positive breast cancer. PMID: 27448983
8. The present results suggest a role for NHERF1 in the progression of breast cancer mediated by the nuclear distribution of the NHERF1 protein, as determined by the truncation or key site mutation of the PDZ-I domain. PMID: 27097111
9. NF2 localizes in nucleus when Ser518 is not phosphorylated, while phosphorylated form is present in cytoplasm and plasma membrane. Data suggest that binding of NF2 to TIMAP and EBP50 is critical in nuclear localization of NF2. (NF2 = neurofibromin 2; TIMAP = TGF-beta-inhibited membrane-associated protein; EBP50 = Ezrin-Radixin-Mosein binding phosphoprotein 50) PMID: 27871951
10. NHERF1/EBP50 encompasses the regulation of several major signaling pathways engaged in cancer, including the receptor tyrosine kinases PDGFR and EGFR, PI3K/PTEN/AKT and Wnt-beta-catenin pathways. PMID: 28068322
11. Data demonstrated that EBP50 overexpression inhibited the adhesion and migration of breast and cervical cancer cell lines and suppressed MMP-2 activity in these cells; while its knockdown promoted cell adhesion and migration, and enhanced the activity of MMP-2.This study reveals the anti-metastatic effect and a new mechanism of EBP50 action in breast and cervical cancer cells. PMID: 28739728
12. Data provide evidence for an important role of NHERF1 in the epithelial-mesenchymal transition of non-small-cell lung cancer cells, as well as migration. PMID: 28739734
13. This interaction is promoted by EPAC1 activation, triggering its translocation to the plasma membrane and binding to NHERF1. Our findings identify a new CFTR-interacting protein and demonstrate that cAMP activates CFTR through two different but complementary pathways - the well-known PKA-dependent channel gating pathway and a new mechanism regulating endocytosis that involves EPAC1. PMID: 27206858
14. we show here that NHERF1 loss from the PM is oncogenic in vivo through its influence on Wnt-APC-beta-catenin and Hippo-YAP pathways. PMID: 27566107
15. High EBP50 expression is associated with cervical cancer. PMID: 27509846
16. Using neutron spin echo spectroscopy (NSE), the authors show salt-concentration-dependent excitation of nanoscale motion at the tip of the C-terminal tail in the phosphomimic S339D/S340D mutant. PMID: 28285124
17. data highlight sequences in PTHR that contribute to NHERF1 interaction and can be altered to prevent phosphorylation-mediated inhibition PMID: 28376304
18. NHERF1 acts with PMCA2 to regulate HER2 signaling and membrane retention in breast cancers PMID: 28235801
19. COOH-terminal sequences mediate enhanced NHERF1 interaction and facilitate the localization of CFTR. PMID: 27793802
20. Na/H exchanger regulatory factor 1 (NHERF1) expression Is downregulated in cisplatin-resistant HeLa cells. PMID: 28085111
21. Wild-type NHERF1 acts as a tumour suppressor, while NHERF1 A190D mutation abolishes the tumour-suppressive effect in cancer cells, due to A190D mutation-mediated nuclear NHERF1 translocation and induction of YAP phosphorylation. PMID: 28011475
22. This study sheds light on a possible therapeutic strategy targeting at the aberrant nuclear expression of EBP50 without affecting the normal physiological function of EBP50 at other subcellular localization. PMID: 26862730
23. A novel NHERF1 mutation in human breast cancer cells inactivates inhibition of EGFR signaling by NHERF1 promoting disease progression. PMID: 26977012
24. Results showed that EBP50 specifically scaffolds the interaction of PTEN with EGFR and enhances the inhibition of PTEN on EGF-induced AKT activation. These results elucidated a novel mechanism regulating EGF-induced AKT signaling. PMID: 26531778
25. autosomal-dominant polycystic kidney disease is associated with a decrease in NHERF1 protein and mRNA levels. PMID: 26142394
26. Interaction of ABCB4 with EBP50 through its PDZ-like motif plays a critical role in the regulation of ABCB4 expression and stability at the canalicular plasma membrane. PMID: 26789121
27. This study identified alpha-actinin-4 as a novel NHERF1 interaction partner and provided new insights into the regulatory mechanism of the actin cytoskeleton by NHERF1. PMID: 26432781
28. deuterium uptake profiles of isolated PDZ1 and PDZ2 were similar to those of full-length EBP50. Interestingly, PDZ1 was more dynamic than PDZ2, and these PDZ domains underwent different conformational changes upon ligand binding PMID: 25789870
29. Nuclear NHERF1 expression is associated with drug resistance in in advanced gastric cancer patients treated with epirubicin/oxaliplatin/capecitabine first line chemotherapy. PMID: 26126066
30. These data highlight a role for NHERF1 in ependymal morphogenesis with direct application to the diagnosis of ependymal tumors. PMID: 25775275
31. activation of the Nherf1-PP1alpha-TAZ pathway in osteoblasts is targeted by histone deacetylase inhibitors PMID: 26491017
32. conclude that FNACs provide useful material for detection of NHERF1 localization and expression, and that high nuclear NHERF1 expression may be a potential marker of aggressiveness in NSCLC PMID: 25744438
33. A molecular switch in the scaffold NHERF1 enables misfolded CFTR to evade the peripheral quality control checkpoint. PMID: 25990958
34. the activation of PTEN and NHERF-1 may impede the evolution of macrophages beyond the M1 into M2 phenotype in tumor microenvironment. PMID: 25756512
35. EBP50 expression was decreased in esophageal squamous cell carcinoma. PMID: 25555876
36. PDZK1 and NHERF1 regulate the transport function of OATP1A2 by modulating protein internalization via a clathrin-dependent pathway and by enhancing protein stability. PMID: 24728453
37. NHERF1 is essential for maintaining the localization and function of Mrp-2. PMID: 25163515
38. These findings reveal an essential role of NHERF1 in epithelial morphogenesis and polarity and validate this 3D system for modeling the molecular changes observed in CRC. PMID: 24862762
39. New conformational state of NHERF1-CXCR2 signaling complex captured by crystal lattice trapping. PMID: 24339979
40. the structural basis for the PDZ domain-mediated NHERF1-CXCR2 interaction and probable modes of PDZ domain dimerization PMID: 24768637
41. Results show that the loss of nuclear NHERF1 expression is associated with reduced survival, and the link between nuclear NHERF1 and ER expression may serve as a prognostic marker for the routine clinical management of breast cancer patients. PMID: 24201803
42. This study provides an understanding of the structural basis for the PDZ-mediated NHERF1-PLCbeta3 interaction that could prove valuable in selective drug design against CXCR2-related cancers. PMID: 24642259
43. cdc2/cyclin B-mediated EBP50 phosphorylation may play a role in the regulation of various cell functions by affecting actin cytoskeleton reorganization. PMID: 23775624
44. The overexpression of EBP50 could inhibit the growth of hepatocellular carcinoma cells and promote apoptosis by modulating beta-catenin, E-cadherin. PMID: 23483729
45. Breast cancer-derived K172N, D301V mutations abolish Na+/H+ exchanger regulatory factor 1 inhibition of platelet-derived growth factor receptor signaling PMID: 24012959
46. Taken together, these data suggest that NHERF-1 plays a more central role in cell proliferation by modulating Gq-mediated signalling pathways. PMID: 23454118
47. The study shows that ligand-induced structural and dynamic changes coupled with sequence variation at the putative PDZ binding site dictate ligand selectivity and binding affinity of the two PDZ domains of NHERF1. PMID: 23583913
48. Our results suggest that EBP50 may function as a potential tumor suppressor PMID: 22622406
49. EBP50 can suppress EGF-induced proliferation of breast cancer cells by inhibiting EGFR phosphorylation and blocking EGFR downstream signaling in breast cancer cells. PMID: 22476347
50. altered expression of peroxiredoxin 1 and radixin-moesin-binding phosphoprotein 50 may be used as prognostic markers in cholangiocarcinoma. PMID: 22446018

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HGNC: 11075

OMIM: 604990

KEGG: hsa:9368

STRING: 9606.ENSP00000262613

UniGene: Hs.724482