Recombinant Human Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2)

Code CSB-YP866332HU
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Source Yeast
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Code CSB-EP866332HU
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Source E.coli
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Code CSB-EP866332HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866332HU
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Source Baculovirus
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Code CSB-MP866332HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NMNAT2
Uniprot No.
Alternative Names
C1orf15; KIAA0479; MGC2756; NaMN adenylyltransferase 2; Nicotinamide mononucleotide adenylyltransferase 2; Nicotinamide nucleotide adenylyltransferase 2; Nicotinate-nucleotide adenylyltransferase 2; NMN adenylyltransferase 2; NMNA2_HUMAN; NMNAT 2; Nmnat2; PNAT 2; PNAT2; Pyridine nucleotide adenylyltransferase 2
Species
Homo sapiens (Human)
Expression Region
1-307
Target Protein Sequence
MTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMC QLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ NETPQPIYQN SNVATKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIP GLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHG DGHVVDYLSQ PVIDYILKSQ LYINASG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor. Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons.
Gene References into Functions
  1. analyses found that rs10494561, located in the intron region within NMNAT2, was associated with the severity of the prodromal symptoms of psychosis implicitly, mediated through the volume of the left hemisphere of the superior frontal region PMID: 28544218
  2. Data indicate a chaperone function of nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2), independent from its enzymatic activity, and NMNAT2 complexes with heat shock protein 90 (HSP90) to refold aggregated protein substrates. PMID: 27254664
  3. We confirmed association at NMNAT2 and identified independent SMG7 association with systemic lupus erythematosus in European American and Amerindian/Hispanic ancestries. PMID: 26783109
  4. NMNAT2 might participate in tumorigenesis of CRC. PMID: 27218101
  5. Knockdown of NMNAT-2 significantly reduces cellular NAD(+) levels and protects cells from p53-dependent cell death upon DNA damage, suggesting an important functional role of NMNAT-2 in p53-mediated signaling. PMID: 24552824
  6. Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) is essential for axon growth and survival. PMID: 24840802
  7. Overexpression of NMNAT2 in colorectal cancer cells renders them sensitive to Tiazofurin antineoplastic action. PMID: 23764899
  8. affects tau phosphorylation by regulating PP2A activity PMID: 23579329
  9. Decreased endogenous NMNAT2 function caused by reduced CREB signaling during pathological insults may be one of underlying mechanisms for neuronal death in tauopathies PMID: 22027994
  10. that overexpression of Nmnat2 in M-cells significantly delayed axon degeneration in vivo PMID: 20857515
  11. Nmnat2 is a neuronal protein peripherally attached to membranes via palmitoylation and suggest that Nmnat2 is transported to synaptic terminals via an endosomal pathway. PMID: 20943658
  12. analysis of isoform-specific targeting and interaction domains in human nicotinamide mononucleotide adenylyltransferases PMID: 20388704
  13. cloning, isolation and kinetics PMID: 12359228
  14. Nicotinamide 5'-mononucleotide adenylyltransferase-2 is markedly expressed in the islets of Langerhans. PMID: 14516279
  15. NMNAT1 is a nuclear protein, whereas NMNAT2 and -3 are localized to the Golgi complex and the mitochondria PMID: 16118205
  16. ATP binds before NMN with Golgi apparatus NMNAT2. NMNH conversion to NADH by NMNAT1 and NMNAT3 occurs at similar rates, conversion by NMNAT2 is much slower. PMID: 17402747

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Subcellular Location
Golgi apparatus membrane; Lipid-anchor. Cytoplasmic vesicle membrane; Lipid-anchor. Cytoplasm. Cell projection, axon.
Protein Families
Eukaryotic NMN adenylyltransferase family
Tissue Specificity
Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans.
Database Links

HGNC: 16789

OMIM: 608701

KEGG: hsa:23057

STRING: 9606.ENSP00000287713

UniGene: Hs.497123

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