Recombinant Human Nuclear factor 1 A-type (NFIA)

Code CSB-YP619635HU
MSDS
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Source Yeast
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Code CSB-EP619635HU
MSDS
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Source E.coli
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Code CSB-EP619635HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619635HU
MSDS
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Source Baculovirus
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Code CSB-MP619635HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NFIA
Uniprot No.
Alternative Names
1110047K16Rik; 9430022M17Rik; CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; FLJ39164; NF I/A; NF-I/A; NF1-A; NFI A; NFI L; NFI-A; NFIA; NFIA_HUMAN; Nuclear factor 1 A type; Nuclear factor 1 A-type; Nuclear factor 1/A; Nuclear factor I/A; TGGCA binding protein; TGGCA-binding protein
Species
Homo sapiens (Human)
Expression Region
1-509
Target Protein Sequence
MYSPLCLTQD EFHPFIEALL PHVRAFAYTW FNLQARKRKY FKKHEKRMSK EEERAVKDEL LSEKPEVKQK WASRLLAKLR KDIRPEYRED FVLTVTGKKP PCCVLSNPDQ KGKMRRIDCL RQADKVWRLD LVMVILFKGI PLESTDGERL VKSPQCSNPG LCVQPHHIGV SVKELDLYLA YFVHAADSSQ SESPSQPSDA DIKDQPENGH LGFQDSFVTS GVFSVTELVR VSQTPIAAGT GPNFSLSDLE SSSYYSMSPG AMRRSLPSTS STSSTKRLKS VEDEMDSPGE EPFYTGQGRS PGSGSQSSGW HEVEPGMPSP TTLKKSEKSG FSSPSPSQTS SLGTAFTQHH RPVITGPRAS PHATPSTLHF PTSPIIQQPG PYFSHPAIRY HPQETLKEFV QLVCPDAGQQ AGQVGFLNPN GSSQGKVHNP FLPTPMLPPP PPPPMARPVP LPVPDTKPPT TSTEGGAASP TSPTYSTPST SPANRFVSVG PRDPSFVNIP QQTQSWYLG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Gene References into Functions
  1. The consistent overlap in clinical presentation provides further evidence of the critical role of NFIA haploinsufficiency in the development of the 1p32-p31 microdeletion syndrome phenotype. PMID: 28941020
  2. Data define a previously unknown nuclear factor I-A-nuclear factor-kappaB feed-forward regulation that may contribute to glioblastoma cell survival. PMID: 27994064
  3. Studies indicate the role of nuclear factor one (NFIs) as epigenetic regulators in cancer. PMID: 28076901
  4. Altogether, these results demonstrated that miR-370 suppressed hepatitis B virus gene expression and replication through repressing NFIA expression, which stimulates hepatitis B virus replication via direct regulation on hepatitis B virus Enhancer I activities. PMID: 27664977
  5. We verified that NFIA binds to the IGFBP2 promoter and transcriptionally enhances IGFBP2 expression levels. We identified that NFIA-mediated IGFBP2 signaling pathways are involved in miR-302b-induced glioma cell death. PMID: 28323865
  6. miR-191 was upregulated in patients with middle- and late-stage NSCLC, and in NSCLC cell lines, under mild hypoxic conditions. miR-191 promoted the proliferation and migration of NSCLC under chronic hypoxic conditions, and this promotion may be associated with its targeting of NFIA. PMID: 28075452
  7. Dihydrocapsaicin can significantly decrease proinflammatory cytokines through enhancing NFIA and inhibiting NF-kappaB expression PMID: 27267730
  8. These results demonstrated that RP5833A20.1 inhibited tumor cell proliferation, induced apoptosis and inhibited cellcycle progression by suppressing the expression of NFIA in U251 cells. PMID: 27779670
  9. NFI-A is involved in the miR-21-induced expression of IL-10 in B cells in nasopharyngeal carcinoma; Il-10 is capable of suppressing CD8+ T-cell activities. PMID: 25544502
  10. microRNA-136 targeted and degraded NFIA, which induced the release of microRNA-223, promoting CD11b expression. Direct base pairing occurs between miR-136 and the 3' UTR of NFIA mRNA. PMID: 26329426
  11. this family also carried a microdeletion affecting solely the NFIA gene, this study substantiates the importance of this gene in craniofacial development. PMID: 25714559
  12. TGF-beta-mediated suppression of ANT2 through NF1/Smad4 complexes contributes to oxidative stress and DNA damage during induction of cellular senescence. PMID: 25220407
  13. a strong candidate gene for asthma and allergic rhinitis PMID: 24560411
  14. RP5-833A20.1/miR-382-5p/NFIA pathway was essential to the regulation of cholesterol homeostasis and inflammatory reactions. PMID: 25265644
  15. This report presents the first case of an intragenic deletion within the NFIA gene that is still consistent with classic clinical phenotypes present in previously reported cases of chromosome 1p31.3 related deletion. PMID: 24462883
  16. High nuclear factor IA expression is associated with glioblastomas. PMID: 24305710
  17. These studies represent the first characterization of miR-223/NFIA axis function in glioma PMID: 23946414
  18. These data suggest that genetic variants in the NF1A gene region may predispose to seasonal patterned of mania in bipolar disorder. PMID: 22925353
  19. NFIA expression in white matter lesions of human newborns with neonatal HIE, as well active MS lesions in adults, revealed that it is similarly expressed in oligodendrocyte progenitors and not oligodendrocytes. PMID: 22807310
  20. Role in the expression mechanism of hNaPi-IIb gene transcription. PMID: 15458926
  21. nuclear factor I has a role in the intrinsic control of cerebellar granule neuron gene expression PMID: 15466411
  22. Distribution of nuclear factor I binding sites correlate with Z-DNA forming regions in human chromosome 22. PMID: 15598822
  23. Data show that human granulocytic differentiation is controlled by a regulatory circuitry involving miR-223 and two transcriptional factors, NFI-A and C/EBPalpha. PMID: 16325577
  24. The NF1-A transcription factor plays an important role in the transcriptional activation of the TR2 orphan receptor gene expression via a promoter activating cis-element. PMID: 17010934
  25. The mouse Nfia mutant phenotype and the common features among five human cases indicate that NFIA haploinsufficiency contributes to a novel human central nervous system malformation syndrome that can also include ureteral and renal defects. PMID: 17530927
  26. NFI family of transcription factors plays a key role in the regulation of both the B-FABP and GFAP genes in malignant glioma cells. PMID: 19540848
  27. in early hematopoiesis, the NFI-A expression level acts as a novel factor channeling HPCs into either the E or G lineage PMID: 19542302
  28. Disruption of the Nfia gene in the mouse causes perinatal lethality, agenesis of the corpus callosum and hydrocephalus. PMID: 10518556

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Involvement in disease
Brain malformations with or without urinary tract defects (BRMUTD)
Subcellular Location
Nucleus.
Protein Families
CTF/NF-I family
Database Links

HGNC: 7784

OMIM: 600727

KEGG: hsa:4774

STRING: 9606.ENSP00000360231

UniGene: Hs.740757

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