Recombinant Human Nuclear transcription factor Y subunit gamma (NFYC)

Code CSB-YP613431HU
MSDS
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Source Yeast
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Code CSB-EP613431HU
MSDS
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Source E.coli
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Code CSB-EP613431HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP613431HU
MSDS
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Source Baculovirus
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Code CSB-MP613431HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NFYC
Uniprot No.
Alternative Names
CAAT box DNA binding protein subunit C; CAAT box DNA-binding protein subunit C; CBF C; CBFC; CCAAT binding factor subunit C; CCAAT transcription binding factor subunit gamma; DKFZp667G242; FLJ45775; H1TF2A; HAP5; histone H1 transcription factor large subunit 2A; HSM; NF-YC; NFYC; NFYC_HUMAN; Nuclear transcription factor Y gamma; Nuclear transcription factor Y subunit C; Nuclear transcription factor Y subunit gamma; OTTHUMP00000009207; OTTHUMP00000009208; OTTHUMP00000009209; OTTHUMP00000009210; OTTHUMP00000009211; OTTHUMP00000009212; OTTHUMP00000009213; OTTHUMP00000009215; OTTHUMP00000009217; OTTHUMP00000231028; Transactivator HSM 1/2; transactivator HSM-1; Transactivator HSM-1/2
Species
Homo sapiens (Human)
Expression Region
1-458
Target Protein Sequence
MSTEGGFGGT SSSDAQQSLQ SFWPRVMEEI RNLTVKDFRV QELPLARIKK IMKLDEDVKM ISAEAPVLFA KAAQIFITEL TLRAWIHTED NKRRTLQRND IAMAITKFDQ FDFLIDIVPR DELKPPKRQE EVRQSVTPAE PVQYYFTLAQ QPTAVQVQGQ QQGQQTTSST TTIQPGQIII AQPQQGQTTP VTMQVGEGQQ VQIVQAQPQG QAQQAQSGTG QTMQVMQQII TNTGEIQQIP VQLNAGQLQY IRLAQPVSGT QVVQGQIQTL ATNAQQGQRN ASQGKPRRCL KETLQITQTE VQQGQQQFSQ FTDGQRNSVQ QARVSELTGE AEPREVKATG NSTPCTSSLP TTHPPSHRAG ASCVCCSQPQ QSSTSPPPSD ALQWVVVEVS GTPNQLETHR ELHAPLPGMT SLSPLHPSQQ LYQIQQVTMP AGQDLAQPMF IQSANQPSDG QAPQVTGD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Gene References into Functions
  1. NF-YC was significantly increased in gliomas. Knockdown of NF-YC reduced brain tumor size in vivo. NF-YC played as an independent predictor of patient survival. PMID: 27495011
  2. p53 negatively regulates the expression of the PRR11-SKA2 bidirectional transcription unit through NF-Y, suggesting that the inability to repress the PRR11-SKA2 bidirectional transcription unit after loss of p53 might contribute to tumorigenesis. PMID: 28257042
  3. TAF12 and NFYC are transcription factors that regulate the epigenome, whereas RAD54L plays a central role in DNA repair PMID: 25965574
  4. the expression of the adipogenic differentiation genes fatty acid binding protein-4, adiponectin, and leptin and the formation of fat droplets were impaired. PMID: 23657974
  5. NFY-C expression was elevated in colorectal adenocarcinomas; moreover, NFY-C mRNA levels correlated with time to disease progression, while NFY-C protein expression was significantly higher in metastatic disease PMID: 22104449
  6. Sp1, NF-Y and FOXO transcription factors are involved in the regulation of LKB1 transcription. PMID: 22412893
  7. NF-YC functions as a new corepressor of agonist-bound mineralocorticoid receptor via alteration of aldosterone-induced MR conformation PMID: 20054001
  8. role in repressing Smad2 and Smad3 transactivating activity PMID: 12023901
  9. C/EBPbeta contributes to the induction of hepatocyte replication in response to HGF in rodents, which is likely due to post-translational modifications. PMID: 15922473
  10. Expression levels of NF-Y target genes changed by CDKN1B correlate with clinical prognosis in multiple neoplasms. PMID: 19559782
  11. NF-YC complexity is generated by dual promoters and alternative splicing PMID: 19690168

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Subcellular Location
Nucleus.
Protein Families
NFYC/HAP5 subunit family
Database Links

HGNC: 7806

OMIM: 605344

KEGG: hsa:4802

STRING: 9606.ENSP00000396620

UniGene: Hs.713051

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