Recombinant Human Omega-amidase NIT2 (NIT2)

Code CSB-YP882075HU
MSDS
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Source Yeast
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Code CSB-EP882075HU
MSDS
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Source E.coli
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Code CSB-EP882075HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882075HU
MSDS
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Source Baculovirus
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Code CSB-MP882075HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
CUA002; MGC111199; NIT 2; Nit protein 2; NIT2; NIT2_HUMAN; Nitrilase family member 2; Nitrilase homolog 2; Omega amidase NIT2; Omega-amidase NIT2
Species
Homo sapiens (Human)
Expression Region
1-276
Target Protein Sequence
MTSFRLALIQ LQISSIKSDN VTRACSFIRE AATQGAKIVS LPECFNSPYG AKYFPEYAEK IPGESTQKLS EVAKECSIYL IGGSIPEEDA GKLYNTCAVF GPDGTLLAKY RKIHLFDIDV PGKITFQESK TLSPGDSFST FDTPYCRVGL GICYDMRFAE LAQIYAQRGC QLLVYPGAFN LTTGPAHWEL LQRSRAVDNQ VYVATASPAR DDKASYVAWG HSTVVNPWGE VLAKAGTEEA IVYSDIDLKK LAEIRQQIPV FRQKRSDLYA VEMKKP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively.
Gene References into Functions
  1. Downregulation of NIT2 inhibits colon cancer cell proliferation and induces cell cycle arrest through the caspase-3 and PARP pathways PMID: 25738796
  2. Human NIT1/2 are not involved in the metabolism of vildagliptin. PMID: 25008847
  3. The activity and substrate specificity of human Nit2 was determined with alpha-ketoglutaramate and succinamate as substrates. PMID: 22674578
  4. Genotype analysis in 4 types of tumor tissue showed allelic imbalance surrounding NIT2 locus; results show that NIT2 plays an important role in cell growth inhibition & links to malignancies, suggesting Nit2 may be a potential tumor suppressor candidate PMID: 17488281
Subcellular Location
Cytoplasm.
Protein Families
Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family
Tissue Specificity
Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Database Links

HGNC: 29878

KEGG: hsa:56954

STRING: 9606.ENSP00000377696

UniGene: Hs.439152

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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