Recombinant Human Otolin-1 (OTOL1)

Code	CSB-YP407389HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP407389HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP407389HU-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP407389HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP407389HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

OTOL1

Uniprot No.

A6NHN0

Alternative Names

OTOL1; Otolin-1

Species

Homo sapiens (Human)

Expression Region

24-477

Target Protein Sequence

KTTPHTK FTKKSEEREM PKGLKPSSGP PPEEEETLFT EMAEMAEPIT KPSALDSVFG TATLSPFENF TLDPADFFLN CCDCCSPVPG QKGEPGETGQ PGPKGEAGNL GIPGPPGVVG PQGPRGYKGE KGLKGERGDQ GVPGYPGKPG AQGEPGPKGD KGNIGLGGVK GQKGSKGDTC GNCTKGEKGD QGAMGSPGLH GGPGAKGEKG EMGEKGEMGD KGCCGDSGER GGKGQKGEGG MKGEKGSKGD SGMEGKSGRN GLPGAKGDPG IKGEKGELGP PGLLGPTGPK GDIGNKGVRG PTGKKGSRGF KGSKGELARV PRSAFSAGLS KPFPPPNIPI KFEKILYNDQ GNYSPVTGKF NCSIPGTYVF SYHITVRGRP ARISLVAQNK KQFKSRETLY GQEIDQASLL VILKLSAGDQ VWLEVSKDWN GVYVSAEDDS IFTGFLLYPE ETSGISP

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Collagen-like protein specifically expressed in the inner ear, which provides an organic scaffold for otoconia, a calcium carbonate structure in the saccule and utricle of the ear. Acts as a scaffold for biomineralization: sequesters calcium and forms interconnecting fibrils between otoconia that are incorporated into the calcium crystal structure. Together with OC90, modulates calcite crystal morphology and growth kinetics.

Gene References into Functions

An expression and purification procedure for the C1q-like domain of human otolin-1 was developed. It forms stable trimers in solution in the presence of calcium ions. C1q-like domains were stabilized by the calcium ions. PMID: 29076638
calcium carbonate crystal growth data demonstrate that Otolin-1 and OC90 modulate in vitro calcite crystal morphology but neither protein is sufficient to produce the shape of otoconia PMID: 24748133
Identification and functional characterization of a similar protein in zebrafish. PMID: 15905077

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

OTOL1 family

Database Links

HGNC: 34071

KEGG: hsa:131149

STRING: 9606.ENSP00000330808

UniGene: Hs.585021

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