Recombinant Human PAX-interacting protein 1 (PAXIP1), partial

Code CSB-YP761652HU
MSDS
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Source Yeast
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Code CSB-EP761652HU
MSDS
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Source E.coli
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Code CSB-EP761652HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP761652HU
MSDS
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Source Baculovirus
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Code CSB-MP761652HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAXIP1
Uniprot No.
Alternative Names
CAGF 28; CAGF 29; CAGF28; CAGF29; FLJ41049; PACIP 1; PACIP1; PAX interacting (with transcription activation domain) protein 1; PAX interacting protein 1; PAX transactivation activation domain-interacting protein; PAX transcription activation domain interacting protein 1 like; PAX-interacting protein 1; PAXI1_HUMAN; PAXIP 1; PAXIP 1L; paxip1; PAXIP1 protein; PAXIP1L; Protein encoded by CAG trinucleotide repeats; PTIP; TNRC 2; TNRC2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Proposed to recruit PAGR1 to sites of DNA damage and the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probably independent of MLL-containing histone methyltransferase (HMT) complexes. However, this function has been questioned. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by KMT2D/MLL2. During immunoglobulin class switching in activated B-cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes. Conflictingly, its function in transcriptional regulation during immunoglobulin class switching is reported to be independent of the MLL2/MLL3 complex.
Gene References into Functions
  1. PAXIP1 and 53BP1 protein levels followed gene expression results, i.e., are intrinsically correlated, and also reduced in more advanced breast cancer tumors. PMID: 28475402
  2. PAXIP1 is involved in sensitizing lung cancer cells to the WEE1 inhibitor AZD1775 in combination with platinum-based treatment. PMID: 27196765
  3. PTIP promotes double-strand break repair through a direct role in homologous recombination. PMID: 20088963
  4. PPM1B can dephosphorylate the Pax2 activation domain and displace the adaptor protein PTIP, thus inhibiting H3K4 methylation and gene activation. PMID: 25631048
  5. uncovered a nuclease, Artemis, as a PTIP-binding protein PMID: 25512557
  6. PITX2 and PTIP co-occupy the promoter of the PITX2's transcriptional target. PMID: 24486544
  7. Polyglutamine expansion AR sequesters PTIP to attenuate DNA repair and increase genomic instability. PMID: 22736030
  8. a new clue to identify the role of PTIP in DNA damage pathway PMID: 22064073
  9. PTIP controls expression of PPARgamma and C/EBPalpha, the two principal adipogenic transcription factors, and is therefore required for adipogenesis. PMID: 19583951
  10. PTIP (PAXIP1) is a strong inhibitor of the trans-activation activities of Pax2A and Pax2B on the glucagon gene promoter and p8 binding to PTIP prevents inhibition of this promoter. PMID: 11940591
  11. identified tandem BRCT (BRCA1 carboxyl-terminal) domains in PTIP and in BRCA1 as phosphoserine- or phosphothreonine-specific binding modules that recognize substrates phosphorylated by the kinases ATM and ATR in response to gamma-irradiation PMID: 14576432
  12. hPTIP (PAXIP1) shows increased association with 53BP1 in response to ionizing radiation but not in response to other DNA-damaging agents. PMID: 15456759
  13. Determination of a single ATM-phosphorylated residue in 53BP1--Ser25--that is required for binding of 53BP1 to hPTIP. PMID: 17690115
  14. PTIP depletion from mammalian cells by RNAi reduced PCNA ubiquitination in response to DNA damage, and also decreased the recruitment to chromatin of polymerase eta and the recombination protein Rad51 PMID: 18353733
  15. subset of PTIP.PA1 complex is recruited to DNA damage sites via the RNF8-dependent pathway and is required for cell survival in response to DNA damage. PMID: 19124460
  16. PTIP regulates 53BP1-dependent signaling pathway following DNA damage PMID: 19414588
  17. Without DNA damage agent treatment, endogenous PTIP associates with MLL3- and MLL4-containing histone H3 K4 methyltransferase complex that also contains ASH2L, RBBP5, WDR5, hDPY-30, NCOA6, PA1 and JmjC domain-containing putative histone demethylase UTX. PMID: 17500065

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Subcellular Location
Nucleus matrix. Chromosome.
Database Links

HGNC: 8624

OMIM: 608254

KEGG: hsa:22976

STRING: 9606.ENSP00000384048

UniGene: Hs.443881

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