Recombinant Human PH and SEC7 domain-containing protein 1 (PSD), partial

Code CSB-YP018842HU
MSDS
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Source Yeast
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Code CSB-EP018842HU
MSDS
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Source E.coli
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Code CSB-EP018842HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018842HU
MSDS
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Source Baculovirus
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Code CSB-MP018842HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PSD
Uniprot No.
Alternative Names
PSD; EFA6; EFA6A; KIAA2011; PSD1; TYL; PH and SEC7 domain-containing protein 1; Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6; Exchange factor for ARF6; Exchange factor for ARF6 A; Pleckstrin homology and SEC7 domain-containing protein 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide exchange factor for ARF6. Induces cytoskeletal remodeling.
Gene References into Functions
  1. Arf GTPases are likely to be locally activated by EFA6 and in turn targeted to the Flemming body to complete cytokinesis. PMID: 26330566
  2. PSD expression might be a useful biomarker for epigenetic-based gastric cancer early diagnosis and may lead to the identification of new targets for pharmacological intervention. PMID: 25355626
  3. EFA6 is regulated by a negative feedback loop, which is mediated by an allosteric interaction of Arf6-GTP with a domain of EFA6 and monitors the activation of Arf1 and Arf6 differentially PMID: 25114232
  4. EFA6 participates in activation of Arf6 around the cleavage furrow during cytokinesis. PMID: 23603394
  5. Aberrant methylation of PSD occurs in UC-associated colorectal mucosa, enabling circumvention of Rac1 immune responses governing neutrophil chemotaxis and apoptosis, and plays a pivotal role in the mechanisms underlying UC-associated carcinogenesis. PMID: 22179719
  6. Both levels of accumulation of F-actin and apoptosis were significantly decreased in specimens from UC patients with PSD methylation compared to those without PSD methylation. PMID: 22002136
  7. Pleckstrin is a critical molecule for proinflammatory cytokine secretion in response to elevated advanced glycation end products (AGE) in diabetes. PMID: 17579087

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Subcellular Location
Cell membrane. Cell projection, ruffle membrane. Cleavage furrow.
Protein Families
PSD family
Tissue Specificity
Isoform 2 is expressed in the brain.
Database Links

HGNC: 9507

OMIM: 602327

KEGG: hsa:5662

STRING: 9606.ENSP00000020673

UniGene: Hs.154658

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