Recombinant Human PTB domain-containing engulfment adapter protein 1 (GULP1)

Code CSB-YP887007HU
MSDS
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Source Yeast
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Code CSB-EP887007HU
MSDS
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Source E.coli
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Code CSB-EP887007HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887007HU
MSDS
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Source Baculovirus
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Code CSB-MP887007HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GULP1
Uniprot No.
Alternative Names
CED6; Cell death protein 6 homolog; Engulfment adapter protein; Engulfment adaptor PTB domain containing 1; GULP; GULP; engulfment adaptor PTB domain containing 1; GULP1; GULP1_HUMAN; Protein GULP; PTB domain adapter protein CED 6; PTB domain adapter protein CED-6; PTB domain adaptor protein CED6; PTB domain-containing engulfment adapter protein 1
Species
Homo sapiens (Human)
Expression Region
1-304
Target Protein Sequence
MNRAFSRKKD KTWMHTPEAL SKHFIPYNAK FLGSTEVEQP KGTEVVRDAV RKLKFARHIK KSEGQKIPKV ELQISIYGVK ILEPKTKEVQ HNCQLHRISF CADDKTDKRI FTFICKDSES NKHLCYVFDS EKCAEEITLT IGQAFDLAYR KFLESGGKDV ETRKQIAGLQ KRIQDLETEN MELKNKVQDL ENQLRITQVS APPAGSMTPK SPSTDIFDMI PFSPISHQSS MPTRNGTQPP PVPSRSTEIK RDLFGAEPFD PFNCGAADFP PDIQSKLDEM QEGFKMGLTL EGTVFCLDPL DSRC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May function as an adapter protein. Required for efficient phagocytosis of apoptotic cells. Modulates cellular glycosphingolipid and cholesterol transport. May play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increases cellular levels of GTP-bound ARF6.
Gene References into Functions
  1. This study highlights and provides new implications for the role of GULP1 associated molecular mechanism(s) in COPD-emphysema development or progression. PMID: 28284325
  2. GULP1 only mediates LRP1 transactivation PMID: 23167255
  3. The apoptotic engulfment protein Ced-6 participates in clathrin-mediated yolk uptake in Drosophila egg chambers. PMID: 22398720
  4. GULP expression positively regulates TGF-beta signaling leading to growth inhibition, this may represent an attractive target to achieve TGF-beta responsiveness in ovarian cells. PMID: 22451657
  5. This study identify GULP1 as a novel neuronal amyloid-beta precursor protein interacting protein that alters trafficking and processing of amyloid-beta precursor protein. PMID: 20674096
  6. overexpression of GULP1 enhanced the generation of APP CTFs (C-terminal fragments) and Abeta, whereas knockdown of GULP1 suppressed APP CTFs and Abeta production. PMID: 21486224
  7. the GULP1 gene and the apoptotic engulfment pathway are involved in schizophrenia in subjects of European ancestry PMID: 19721717
  8. Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP). PMID: 11729193
  9. GULP is a likely downstream molecule in the stabilin-2-mediated signaling pathway and plays an important role in stabilin-2-mediated phagocytosis PMID: 18230608
  10. Phosphatidylserine-recognizing SR-BI activates associated GULP; activated GULP induces MAPK phosphorylation; activated MAPK increases GTP-bound Rac1; and activated Rac1 induces a rearrangement of the actin cytoskeleton. PMID: 19122200

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Subcellular Location
Cytoplasm. Note=May associate with the cytoplasmic side of the plasma membrane and early endosomes.
Protein Families
Ced-6 family
Tissue Specificity
Widely expressed. Detected in macrophages, pancreas, kidney, skeletal muscle, heart, colon, intestine, lung, placenta and ovary.
Database Links

HGNC: 18649

OMIM: 608165

KEGG: hsa:51454

STRING: 9606.ENSP00000352047

UniGene: Hs.470887

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