Recombinant Human Paired box protein Pax-1 (PAX1)

Code CSB-YP017487HU
MSDS
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Source Yeast
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Code CSB-EP017487HU
MSDS
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Source E.coli
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Code CSB-EP017487HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017487HU
MSDS
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Source Baculovirus
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Code CSB-MP017487HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAX1
Uniprot No.
Alternative Names
HUP48; OTTHUMP00000030407; Paired box 1; Paired box gene 1; Paired box protein Pax 1; Paired box protein Pax-1; Paired domain gene HuP48; PAX 1; Pax1; PAX1_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-534
Target Protein Sequence
MKFTLGLGSR AWRVSWEGAA AAAAGPGAGG SALRCRAQRV SSPRLGRRGS RLSGALPLCL SRGGGGAQAL PDCAGPSPGH PGHPGARQLA GPLAMEQTYG EVNQLGGVFV NGRPLPNAIR LRIVELAQLG IRPCDISRQL RVSHGCVSKI LARYNETGSI LPGAIGGSKP RVTTPNVVKH IRDYKQGDPG IFAWEIRDRL LADGVCDKYN VPSVSSISRI LRNKIGSLAQ PGPYEASKQP PSQPTLPYNH IYQYPYPSPV SPTGAKMGSH PGVPGTAGHV SIPRSWPSAH SVSNILGIRT FMEQTGALAG SEGTAYSPKM EDWAGVNRTA FPATPAVNGL EKPALEADIK YTQSASTLSA VGGFLPACAY PASNQHGVYS APGGGYLAPG PPWPPAQGPP LAPPGAGVAV HGGELAAAMT FKHPSREGSL PAPAARPRTP SVAYTDCPSR PRPPRGSSPR TRARRERQAD PGAQVCAAAP AIGTGRIGGL AEEEASAGPR GARPASPQAQ PCLWPDPPHF LYWSGFLGFS ELGF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column.
Gene References into Functions
  1. The association between PAX1 and the susceptibility of AIS was successfully replicated in the Chinese population PMID: 29095406
  2. Hypermethylated ZNF582 and PAX1 genes in the oral scrapings collected from adjacent normal oral mucosal sites rather than cancer sites are associated with aggressive progression and poor prognosis of OSCC PMID: 29224816
  3. A novel PAX1 null homozygous mutation in autosomal recessive otofaciocervical syndrome associated with severe combined immunodeficiency has been described in a consanguineous family. PMID: 28657137
  4. PAX1 gene methylation was associated with the transition of CIN I to CIN II/III and CIN II/III to cervical cancer, so that it could be an auxiliary biomarker to estimate the risk of CIN progress. PMID: 28472814
  5. conclude that hypermethylated ZNF582 and PAX1 are effective biomarkers for the detection of oral dysplasia and oral cancer and for the prediction of oral cancer recurrence PMID: 27865370
  6. DNA methylation status of PAX1 showed a relatively good sensitivity and specificity for the detection of ESOPHAGEAL SQUAMOUS CELL CARCINOMA. PMID: 28241446
  7. Paired boxed gene 1 protein expression is a potential histopathology biomarker for the differential diagnosis of malignant and premalignant endometrial lesions. Paired boxed gene 1 is also a potential prognostic marker in cases of endometrial carcinoma. PMID: 27226215
  8. Hypermethylation of PAX1 gene may be highly associated with the development of cervical cancer. PMID: 26552048
  9. meta-analysis support the utility of PAX1 methylation as an auxiliary biomarker in cervical cancer screening PMID: 26234429
  10. Testing PAX1 DNA methylation using oral swabs is a promising method for oral cancer detection. Combined assessments regarding betel nut consumption and DNA methylation can improve OSCC screening PMID: 23907469
  11. PAX1 methylation hallmarks a potential diagnostic value for cervical cancer screening in Asians PMID: 26642709
  12. analysis of a PAX1 enhancer locus that is associated with susceptibility to idiopathic scoliosis in females PMID: 25784220
  13. PAX1 and SOX1 DNA methylation correlate with a cervical intraepithelial neoplasia diagnosis. PMID: 24799352
  14. PAX1 methylation is associated with cervical cancer. PMID: 24844223
  15. PAX1 methylation is associated with high-grade squamous intraepithelial lesions. PMID: 24568514
  16. Cervical adenocarcinoma cells carry aberrantly high methylation rates of PAX1, commonly methylated in squamous cell carcinomas. PMID: 24407576
  17. We identified PAX1 as a novel disease-causing gene for otofaciocervical syndrome and show that the pooling of DNA from affected individuals can reduce the number of putative disease-causing homozygous variants in consanguineous families. PMID: 23851939
  18. PAX1 hypermethylation is associated with cervical cancer. PMID: 20442585
  19. There was a significant reduction in PAX1 expression in fetuses with the Jarcho-Levin syndrome. PMID: 12833407
  20. No mutations were identified in the PAX1 and MEOX1 exons or flanking intronic sequences, excluding them as likely causative genes for diaphanospondylodysostosis PMID: 17764081

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Involvement in disease
Otofaciocervical syndrome 2 (OTFCS2)
Subcellular Location
Nucleus.
Database Links

HGNC: 8615

OMIM: 167411

KEGG: hsa:5075

STRING: 9606.ENSP00000381499

UniGene: Hs.122310

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