Recombinant Human Phosphofurin acidic cluster sorting protein 1 (PACS1), partial

Code CSB-YP017371HU
MSDS
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Source Yeast
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Code CSB-EP017371HU
MSDS
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Source E.coli
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Code CSB-EP017371HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017371HU
MSDS
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Source Baculovirus
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Code CSB-MP017371HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PACS1
Uniprot No.
Alternative Names
Cytosolic sorting protein PACS1; PACS 1; PACS-1; Pacs1; PACS1_HUMAN; Phosphofurin acidic cluster sorting protein 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Coat protein that is involved in the localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin-coated membrane pits. Involved in HIV-1 nef-mediated removal of MHC-I from the cell surface to the TGN.
Gene References into Functions
  1. Using whole exome sequencing (WES), we identified eight additional individuals with the same p.Arg203Trp missense variant in PACS1 that we demonstrated were de novo in seven individuals with available parental DNA. PMID: 28111752
  2. PCAF and ADA3 transcriptionally regulate PACS1 and PACS1 is a key regulator of BAX/BAK oligomerization and the intrinsic (mitochondrial) pathway to apoptosis. PMID: 28060382
  3. Protein adaptation and the expanding roles of the PACS1 and PACS2 proteins in tissue homeostasis and disease has been summarized. (Review) PMID: 28476937
  4. study showed for the first time that the rs564343 in phosphofurin acidic cluster sorting protein 1(PACS1) was associated with risk of severe obesity in Chinese children PMID: 28564656
  5. Clinical description of the PACS1-related syndrome has been reported in 19 unrelated patients. PMID: 26842493
  6. PACS1 plays an important role in protein sorting for amyloidogenic-burden control via both SORLA-dependent and SORLA-independent mechanisms. PMID: 24001769
  7. Single nucleotide polymorphisms in PAKS1 gene is associated with obesity. PMID: 23563609
  8. Our findings suggest that PACS1 is necessary for the formation of craniofacial structures and that perturbation of its functions results in a specific syndromic ID phenotype. PMID: 23159249
  9. The sites on Nef and the PACS proteins required for their interaction, are identified. PMID: 22496420
  10. PACS-1 along with HIV-1 nef, has a role in downregulating cell surface MHC-I to the trans-Golgi network and usurping the ARF6 endocytic pathway PMID: 12526811
  11. subcellular localization and function of polycystin-2 are directed by phosphofurin acidic cluster sorting protein (PACS)-1 and PACS-2 PMID: 15692563
  12. casein kinase 2 induces PACS-1 binding of nephrocystin and targeting to cilia PMID: 16308564
  13. CK2-activated phosphorylation cascade controlling PACS-1- and GGA3-mediated CI-MPR sorting, is reported. PMID: 16977309
  14. PACS-1 mediates phosphorylation-dependent ciliary trafficking of the CNGB1b in olfactory sensory neurons. PMID: 19710307

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Involvement in disease
Schuurs-Hoeijmakers syndrome (SHMS)
Subcellular Location
Golgi apparatus, trans-Golgi network.
Protein Families
PACS family
Database Links

HGNC: 30032

OMIM: 607492

KEGG: hsa:55690

STRING: 9606.ENSP00000316454

UniGene: Hs.644326

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