Recombinant Human Phytanoyl-CoA dioxygenase domain-containing protein 1 (PHYHD1)

Code CSB-YP716328HU
MSDS
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Source Yeast
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Code CSB-EP716328HU
MSDS
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Source E.coli
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Code CSB-EP716328HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP716328HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP716328HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PHYHD1
Uniprot No.
Alternative Names
MGC16638; OTTHUMP00000022302; OTTHUMP00000022303; OTTHUMP00000022304; OTTHUMP00000022305; OTTHUMP00000199642; PHYD1_HUMAN; Phyhd1; Phytanoyl CoA dioxygenase domain containing 1; Phytanoyl CoA dioxygenase domain containing protein 1; Phytanoyl-CoA dioxygenase domain-containing protein 1
Species
Homo sapiens (Human)
Expression Region
1-291
Target Protein Sequence
MACLSPSQLQ KFQQDGFLVL EGFLSAEECV AMQQRIGEIV AEMDVPLHCR TEFSTQEEEQ LRAQGSTDYF LSSGDKIRFF FEKGVFDEKG NFLVPPEKSI NKIGHALHAH DPVFKSITHS FKVQTLARSL GLQMPVVVQS MYIFKQPHFG GEVSPHQDAS FLYTEPLGRV LGVWIAVEDA TLENGCLWFI PGSHTSGVSR RMVRAPVGSA PGTSFLGSEP ARDNSLFVPT PVQRGALVLI HGEVVHKSKQ NLSDRSRQAY TFHLMEASGT TWSPENWLQP TAELPFPQLY T
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Isoform 1 has alpha-ketoglutarate-dependent dioxygenase activity. Does not show detectable activity towards fatty acid CoA thioesters. Is not expected to be active with phytanoyl CoA. Isoform 2 and isoform 3 probably lack enzyme activity.
Gene References into Functions
  1. PHYHD1A has the double-stranded beta-helix fold and Fe(II) and cosubstrate binding residues characteristic of the 2-oxoglutarate dependent oxygenases and catalyzes the conversion of 2-oxoglutarate to succinate and CO(2) in an iron-dependent manner. PMID: 21530488
Protein Families
PhyH family, PHYHD1 subfamily
Database Links

HGNC: 23396

KEGG: hsa:254295

STRING: 9606.ENSP00000309515

UniGene: Hs.709447

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