Recombinant Human Prion-like protein doppel (PRND)

Code CSB-YP890741HU
MSDS
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Source Yeast
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Code CSB-EP890741HU
MSDS
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Source E.coli
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Code CSB-EP890741HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890741HU
MSDS
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Source Baculovirus
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Code CSB-MP890741HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Prion-like protein doppel; PrPLP; Prion protein 2
Species
Homo sapiens (Human)
Expression Region
26-152aa
Target Protein Sequence
TRGIKHRIKWNRKALPSTAQITEAQVAENRPGAFIKQGRKLDIDFGAEGNRYYEANYWQFPDGIHYNGCSEANVTKEAFVTGCINATQAANQGEFQKPDNKLHQQVLWRLVQELCSLKHCEFWLERG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for normal acrosome reaction and for normal male fertility. Can bind Cu(2+).
Gene References into Functions
  1. results demonstrate that blocking doppel can control VEGF signaling in TECs and selectively inhibit tumor angiogenesis. PMID: 26950422
  2. The association between the APOE, CYP46, PRNP and PRND genes and the profile of neuropsychiatric symptoms in Polish subjects with Alzheimer's disease and mild cognitive impairment, was evaluated. PMID: 22453181
  3. in astrocytomas, Dpl undergoes different molecular processes that might constitute additional helpful tools to characterize the glial tumor progression PMID: 20981146
  4. Cytotoxicity induced by the expression of Dpl and truncated PrP in neural derived cells are closely related with the apoptosis process. PMID: 19728151
  5. expression pattern and biochemical characteristics in human tissues and in Chinese hamster ovary cells transfected with wild-type or variant human Dpl gene constructs PMID: 12200435
  6. Doppel expression is not modified in the brains of patients with Creutzfeldt-Jakob disease. PMID: 12586339
  7. human Doppel fails to interact with itself; Dpl and PrP are not related or are only marginally related with respect to their ligand binding behaviour PMID: 15158907
  8. results demonstrate that Doppel and PrPc co-patch extensively at the plasma membrane PMID: 15203115
  9. Doppel interacts with the full-length laminin receptor precursor protein PMID: 15246873
  10. Doppel is expressed early during ontogenesis, and is found in both germ cells and Sertoli cells. Doppel may play a physiological role in acrosome biogenesis. PMID: 16421231
  11. Extensive differences in PRND gene expression were also found within each grade of malignancy, suggesting that PRND mRNA quantitation might be useful to distinguish astrocytoma subtypes. PMID: 17390034
  12. Study provides the foundation for further study of Dpl biological functions in vitro and in vivo. PMID: 17894227
  13. Results might suggest a potential and functional role for Dpl in tumor cells migratory and morphological behaviours and address to future gene-targeted therapeutic interventions. PMID: 18936526
  14. results suggested that the function of Dpl is antagonistic to PrP rather than synergistic PMID: 19129949

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Prion family
Tissue Specificity
Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level).
Database Links

HGNC: 15748

OMIM: 604263

KEGG: hsa:23627

STRING: 9606.ENSP00000306900

UniGene: Hs.406696

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