Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

NPFF

Uniprot No.

O15130

Research Area

Neuroscience

Alternative Names

FMRFamide-related peptides

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

66-76aa

Target Protein Sequence

SQAFLFQPQRF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

32.8 kDa

Protein Length

Partial

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To achieve the expression of the recombinant Human NPFF protein in e.coli cells, a DNA fragment encoding the Human NPFF protein (66-76aa) is inserted into a plasmid vector, which is then transferred to e.coli cells. After screening positive cells, they are cultured and induced to creat the NPFF protein. The protein features a N-terminal GST tag fusion. The recombinant Human NPFF protein is purified from the cell lysate through affinity purification. The purity of the obtained recombinant Human NPFF protein is greater than 90%, measured by SDS-PAGE.

Target Background

Function

Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels.

Gene References into Functions

Our results...highlight the neuropeptide FF-amide peptide encoding gene (NPFF) as a novel candidate risk gene for both migraine with aura and migraine without aura PMID: 26660531
reduction of NPFF release within the dorsal motor nucleus and ambiguus nucleus could serve as a possible cause of the impairment of cardiac vagal control in hypertensive patients PMID: 21120930
expression of hNPFF2 receptor mRNA was up-regulated by neuropeptide FF PMID: 16336216
neuropeptide FF and micro-opioid receptor association could be a possible molecular basis for anti-opioid activity PMID: 17224450
the findings from the early studies on the analgesic and morphine modulating activities suggested a role for NPFF in pain modulation--review PMID: 17854890

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Subcellular Location

Secreted.

Protein Families

FARP (FMRFamide related peptide) family

Database Links

HGNC: 7901

OMIM: 604643

KEGG: hsa:8620

STRING: 9606.ENSP00000267017

UniGene: Hs.733076

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Code	CSB-EP015981HU
Abbreviation	Recombinant Human NPFF protein, partial
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Pro-FMRFamide-related neuropeptide FF (NPFF), partial

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