Recombinant Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2)

Code CSB-YP017340HU
MSDS
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Source Yeast
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Code CSB-EP017340HU
MSDS
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Source E.coli
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Code CSB-EP017340HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017340HU
MSDS
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Source Baculovirus
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Code CSB-MP017340HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
P4HA2
Uniprot No.
Alternative Names
2-oxoglutarate-4-dioxygenase subunit alpha-2; 4 PH alpha 2; 4-PH alpha-2; C-P4Halpha(II); collagen prolyl 4-hydroxylase alpha(II); P4HA2; P4HA2_HUMAN; procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II; procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2; Procollagen-proline; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Prolyl 4 hydroxylase alpha 2 subunit; Prolyl 4 hydroxylase subunit alpha 2 [Precursor]; Prolyl 4-hydroxylase subunit alpha-2; Prolyl 4-hydroxylase, alpha polypeptide II
Species
Homo sapiens (Human)
Expression Region
22-535
Target Protein Sequence
EFFTSIGHM TDLIYAEKEL VQSLKEYILV EEAKLSKIKS WANKMEALTS KSAADAEGYL AHPVNAYKLV KRLNTDWPAL EDLVLQDSAA GFIANLSVQR QFFPTDEDEI GAAKALMRLQ DTYRLDPGTI SRGELPGTKY QAMLSVDDCF GMGRSAYNEG DYYHTVLWME QVLKQLDAGE EATTTKSQVL DYLSYAVFQL GDLHRALELT RRLLSLDPSH ERAGGNLRYF EQLLEEEREK TLTNQTEAEL ATPEGIYERP VDYLPERDVY ESLCRGEGVK LTPRRQKRLF CRYHHGNRAP QLLIAPFKEE DEWDSPHIVR YYDVMSDEEI ERIKEIAKPK LARATVRDPK TGVLTVASYR VSKSSWLEED DDPVVARVNR RMQHITGLTV KTAELLQVAN YGVGGQYEPH FDFSRNDERD TFKHLGTGNR VATFLNYMSD VEAGGATVFP DLGAAIWPKK GTAVFWYNLL RSGEGDYRTR HAACPVLVGC KWVSNKWFHE RGQEFLRPCG STEVD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Gene References into Functions
  1. Assembly of the elongated collagen prolyl 4-hydroxylase alpha2beta2 heterotetramer around a central alapha2 dimer has been reported. PMID: 28093469
  2. Plasminogen and P4HA2 are involved in vascular remodelling and angiogenesis, suggesting a high relevance of these processes for the pathogenic mechanisms underlying this type of vasculitis PMID: 28041642
  3. RNAsequencing showed that the t(4;5)(q24;q31) resulted in recombination of the genes TBCK on 4q24 and P4HA2 on 5q31.1 with generation of an inframe TBCKP4HA2 and the reciprocal but outofframe P4HA2TBCK fusion transcripts. PMID: 27633981
  4. P4HA2 was identified as a novel causative gene for nonsyndromic high myopia. PMID: 25741866
  5. Suggest the critical role of P4HA2 in breast cancer progression and identify P4HA2 as a potential therapeutic target and biomarker for breast cancer progression. PMID: 24383403
  6. Hypoxia-inducible factor 1 (HIF-1) promotes extracellular matrix remodeling under hypoxic conditions by inducing P4HA1, P4HA2, and PLOD2 expression in fibroblasts. PMID: 23423382
  7. P4HA2 was upregulated in breast tumor cells compared with its adjacent normal tissues. PMID: 22813596
  8. Overexpression of PRDX4 and P4HA2 was significantly associated with lymphatic metastasis in oral cavity squamous cell carcinoma PMID: 21859152
  9. the substrate specificity, binding site and conformation of this enzyme PMID: 12824157
  10. In comparison with healthy cartilage, Osteoarthritis articular chondrocytes exhibit increased in vivo synthesis of collagen prolyl-4-hydroxylase type II, a pivotal enzyme in collagen triple helix formation. PMID: 16877351
  11. HIF-P4H, HIF-1alpha and HIF-2alpha are effective oxygen sensors PMID: 16885164
  12. results show that p53 transcriptionally activates the alpha(II) collagen prolyl-4-hydroxylase [alpha(II)PH] gene, resulting in the extracellular release of antiangiogenic fragments of collagen type 4 and 18 PMID: 16917063

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Involvement in disease
Myopia 25, autosomal dominant (MYP25)
Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
P4HA family
Database Links

HGNC: 8547

OMIM: 600608

KEGG: hsa:8974

STRING: 9606.ENSP00000166534

UniGene: Hs.519568

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