P4HA2 Antibody

Datasheet

Code	CSB-PA017340ESR1HU
Size	US$166
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Image	Western blot All lanes: P4HA2 antibody at 6μg/ml Lane 1: HepG2 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA017340ESR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA017340ESR1HU at dilution of 1:100 Immunoprecipitating P4HA2 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA017340ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA017340ESR1HU (4μg) + Hela whole cell lysate (500μg) Lane 3: Hela whole cell lysate (20μg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) P4HA2 Polyclonal antibody

Uniprot No.

O15460

Target Names

P4HA2

Alternative Names

2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody; 4 PH alpha 2 antibody; 4-PH alpha-2 antibody; C-P4Halpha(II) antibody; collagen prolyl 4-hydroxylase alpha(II) antibody; P4HA2 antibody; P4HA2_HUMAN antibody; procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II antibody; procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2 antibody; Procollagen-proline antibody; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody; Prolyl 4 hydroxylase alpha 2 subunit antibody; Prolyl 4 hydroxylase subunit alpha 2 [Precursor] antibody; Prolyl 4-hydroxylase subunit alpha-2 antibody; Prolyl 4-hydroxylase, alpha polypeptide II antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Prolyl 4-hydroxylase subunit alpha-2 protein (236-535AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Gene References into Functions

Assembly of the elongated collagen prolyl 4-hydroxylase alpha2beta2 heterotetramer around a central alapha2 dimer has been reported. PMID: 28093469
Plasminogen and P4HA2 are involved in vascular remodelling and angiogenesis, suggesting a high relevance of these processes for the pathogenic mechanisms underlying this type of vasculitis PMID: 28041642
RNAsequencing showed that the t(4;5)(q24;q31) resulted in recombination of the genes TBCK on 4q24 and P4HA2 on 5q31.1 with generation of an inframe TBCKP4HA2 and the reciprocal but outofframe P4HA2TBCK fusion transcripts. PMID: 27633981
P4HA2 was identified as a novel causative gene for nonsyndromic high myopia. PMID: 25741866
Suggest the critical role of P4HA2 in breast cancer progression and identify P4HA2 as a potential therapeutic target and biomarker for breast cancer progression. PMID: 24383403
Hypoxia-inducible factor 1 (HIF-1) promotes extracellular matrix remodeling under hypoxic conditions by inducing P4HA1, P4HA2, and PLOD2 expression in fibroblasts. PMID: 23423382
P4HA2 was upregulated in breast tumor cells compared with its adjacent normal tissues. PMID: 22813596
Overexpression of PRDX4 and P4HA2 was significantly associated with lymphatic metastasis in oral cavity squamous cell carcinoma PMID: 21859152
the substrate specificity, binding site and conformation of this enzyme PMID: 12824157
In comparison with healthy cartilage, Osteoarthritis articular chondrocytes exhibit increased in vivo synthesis of collagen prolyl-4-hydroxylase type II, a pivotal enzyme in collagen triple helix formation. PMID: 16877351
HIF-P4H, HIF-1alpha and HIF-2alpha are effective oxygen sensors PMID: 16885164
results show that p53 transcriptionally activates the alpha(II) collagen prolyl-4-hydroxylase [alpha(II)PH] gene, resulting in the extracellular release of antiangiogenic fragments of collagen type 4 and 18 PMID: 16917063

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Involvement in disease

Myopia 25, autosomal dominant (MYP25)

Subcellular Location

Endoplasmic reticulum lumen.

Protein Families

P4HA family

Database Links

HGNC: 8547

OMIM: 600608

KEGG: hsa:8974

STRING: 9606.ENSP00000166534

UniGene: Hs.519568

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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