Recombinant Human Protein IL-40 (C17orf99)

The Recombinant Human C17orf99 protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of C17orf99 gene. This recombinant C17orf99 protein was expressed in the host. The expression region is 21-265aa of the Human C17orf99. In the production, the expression vector contains N-terminal 10xHis tag and C-terminal Myc tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.

Interleukin 40 (IL-40) is a newly identified B cell-associated cytokine implicated in humoral immune responses and B cell homeostasis. As B cells play a pivotal role in autoimmunity. This cytokine was originally described by Catalan-Dibene et al. in 2017 as a small (27 kDa) secreted protein encoded by the C17orf99 gene (chromosome 17 open reading frame 99). Based on its unique structural properties, IL-40 fell into the category of the few so-called “orphan” cytokines. To date, there have been only few studies regarding the C17orf99 gene or its product IL-40 protein. It is well established that C17orf99 or IL-40 are expressed only in mammals and are particularly enhanced in fetal liver, bone marrow, and activated B cells. Very recently, C17orf99 was found down-regulated in the co-culture of human respiratory epithelial cells with macrophages upon treatment with anti-inflammatory cytokine IL-38, suggesting the role of IL-40 in inflammation. The only evidence linking IL-40 to autoimmune inflammation was reported back in 2012 by Zingaretti et al., who preliminary data indicate that IL-40 is released by RA neutrophils undergoing NETosis.