Recombinant Human Protein Mis18-alpha (MIS18A)

Code CSB-YP885766HU
MSDS
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Source Yeast
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Code CSB-EP885766HU
MSDS
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Source E.coli
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Code CSB-EP885766HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885766HU
MSDS
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Source Baculovirus
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Code CSB-MP885766HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MIS18A
Uniprot No.
Alternative Names
MIS18A; C21orf45; C21orf46; FASP1; Protein Mis18-alpha; FAPP1-associated protein 1
Species
Homo sapiens (Human)
Expression Region
1-233
Target Protein Sequence
MAGVRSLRCS RGCAGGCECG DKGKCSDSSL LGKRLSEDSS RHQLLQKWAS MWSSMSEDAS VADMERAQLE EEAAAAEERP LVFLCSGCRR PLGDSLSWVA SQEDTNCILL RCVSCNVSVD KEQKLSKREK ENGCVLETLC CAGCSLNLGY VYRCTPKNLD YKRDLFCLSV EAIESYVLGS SEKQIVSEDK ELFNLESRVE IEKSLTQMED VLKALQMKLW EAESKLSFAT CKS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.
Gene References into Functions
  1. The authors demonstrate that CDK1 controls Mis18 complex recruitment to centromeres by regulating oligomerization of M18BP1 through the Mis18alpha:Mis18beta scaffold. PMID: 28059702
  2. the Mis18 complex contains dual CENP-C recognition motifs that are combinatorially required to generate robust centromeric localization that leads to CENP-A deposition PMID: 27239045
  3. Mis18alpha is important for epidermal cell proliferation and stratification, because it is required for the deposition of CENP-A at the centromeric nucleosomes. PMID: 27670610
  4. Stable binding of Mis18alpha-Mis18beta heterotetramer to centromeres in telophase licenses them for CENP-A deposition. PMID: 26942680
  5. it is suggested that pp5644 physically bind to FASP1 PMID: 15881666
  6. Three human proteins essential for centromere/kinetochore structure and function, hMis18alpha, hMis18beta, and M18BP1, the complex of which is accumulated specifically at the telophase-G1 centromere, are identified.[Mis18alpha, Mis18beta, M18BP1] PMID: 17199038

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Subcellular Location
Nucleus. Chromosome. Chromosome, centromere. Note=Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.
Protein Families
Mis18 family
Tissue Specificity
Detected in testis.
Database Links

HGNC: 1286

KEGG: hsa:54069

STRING: 9606.ENSP00000290130

UniGene: Hs.190518

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