Recombinant Human Protein SGT1 (ECD)

Code CSB-YP007370HU
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Source Yeast
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Code CSB-EP007370HU
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Source E.coli
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Code CSB-EP007370HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007370HU
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Source Baculovirus
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Code CSB-MP007370HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ECD
Uniprot No.
Alternative Names
ecd; ECD_HUMAN; Ecdysoneless homolog; hSGT1; Human suppressor of GCR two; Protein ecdysoneless homolog; SGT 1; SGT1 protein; Suppressor of GCR2
Species
Homo sapiens (Human)
Expression Region
1-644
Target Protein Sequence
MEETMKLATM EDTVEYCLFL IPDESRDSDK HKEILQKYIE RIITRFAPML VPYIWQNQPF NLKYKPGKGG VPAHMFGVTK FGDNIEDEWF IVYVIKQITK EFPELVARIE DNDGEFLLIE AADFLPKWLD PENSTNRVFF CHGELCIIPA PRKSGAESWL PTTPPTIPQA LNIITAHSEK ILASESIRAA VNRRIRGYPE KIQASLHRAH CFLPAGIVAV LKQRPRLVAA AVQAFYLRDP IDLRACRVFK TFLPETRIMT SVTFTKCLYA QLVQQRFVPD RRSGYRLPPP SDPQYRAHEL GMKLAHGFEI LCSKCSPHFS DCKKSLVTAS PLWASFLESL KKNDYFKGLI EGSAQYRERL EMAENYFQLS VDWPESSLAM SPGEEILTLL QTIPFDIEDL KKEAANLPPE DDDQWLDLSP DQLDQLLQEA VGKKESESVS KEEKEQNYDL TEVSESMKAF ISKVSTHKGA ELPREPSEAP ITFDADSFLN YFDKILGPRP NESDSDDLDD EDFECLDSDD DLDFETHEPG EEASLKGTLD NLKSYMAQMD QELAHTCISK SFTTRNQVEP VSQTTDNNSD EEDSGTGESV MAPVDVDLNL VSNILESYSS QAGLAGPASN LLQSMGVQLP DNTDHRPTSK PTKN
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.
Gene References into Functions
  1. The interaction of ECD with RUVBL1, and its CK2-mediated phosphorylation, independent of its interaction with PIH1D1, are important for its cell cycle regulatory function. PMID: 26711270
  2. Ecdysoneless and H-Ras expressing human mammary epithelial cells form tumors in NOD/SCID mice. PMID: 25616580
  3. Ecd is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells. PMID: 22977192
  4. Ecd as a novel marker for breast cancer progression and show that levels of Ecd expression predict poorer survival in Her2/neu overexpressing breast cancer patients. PMID: 22270930
  5. Ecd localizes to both nucleus & cytoplasm & shuttles between the nucleus and cytoplasm; however, it exhibits strong nuclear export. Based on previous yeast studies & evidence provided here, we suggest that Ecd functions as a transcriptional regulator. PMID: 19919181

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
ECD family
Tissue Specificity
Highly expressed in muscle and heart. Over-expressed in pancreatic and breast cancers.
Database Links

HGNC: 17029

OMIM: 616464

KEGG: hsa:11319

STRING: 9606.ENSP00000401566

UniGene: Hs.631822

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