Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SSX2

Uniprot No.

Q16385

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

Cancer/testis antigen 5.2; Cancer/testis antigen family 5, member 2a; Cancer/testis antigen family 5, member 2b; CT5.2; CT5.2A; CT5.2b; HD21; hom mel 40; HOM-MEL-40; MGC119055; MGC15364; MGC3884; Protein SSX2; RP11-552J9.2; sarcoma, synovial, X-chromosome related 2; SSx; SSX2; SSX2-SYT fusion gene, included; SSX2_HUMAN; SSX2B; Synovial sarcoma; synovial sarcoma X breakpoint 2; Synovial sarcoma, X breakpoint 2B; Tumor antigen HOM-MEL-40; X breakpoint 2

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-188aa

Target Protein Sequence

MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGPKRGEHAWTHRLRERKQLVIYEEISDPEEDDE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.6kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human SSX2 covers amino acids 1-188. The theoretical molecular weight of the SSX2 protein is 37.6 kDa. This SSX2 protein is produced using e.coli expression system. Fusion of the N-terminal 6xHis-SUMO tag into the SSX2 encoding gene fragment was conducted, allowing for easier detection and purification of the SSX2 protein in subsequent stages.

The human SSX2 protein functions in transcriptional regulation, playing a role in gene expression control. Understanding SSX2's functions enhances insights into cancer biology, gene regulation, and reproductive processes, offering potential applications in cancer diagnostics, therapy, and reproductive health research. In cancer research, SSX2 is associated with various malignancies, potentially contributing to tumor development. Its involvement in immunotherapy targets provides avenues for cancer treatment. In reproductive biology, SSX2 participates in spermatogenesis.

Target Background

Function

Could act as a modulator of transcription.

Gene References into Functions

We conclude from these findings that SSX2 expression in prostate cancer is not a driver of the epithelial to mesenchymal transition (EMT), but is involved in processes associated with EMT including loss of focal adhesion that may be related to tumor cell dissemination PMID: 27276714
results reveal two important phenotypes of ectopic SSX2 expression that may drive/support tumorigenesis: First, immediate induction of genomic instability, and second, long-term support of tumor cell growth. PMID: 25363656
The tumor-specific pattern of expression of SSX2. PMID: 24681846
primary autoantibodies against intracellular MM-specific tumor antigens SSX-2 and NY-ESO-1 are rare but functional in multiple myeloma patients after allogeneic stem cell transplantation PMID: 25078248
SSX2, SSX3, and SSX4 are expressed in the lung tissue of only 3.5% early-stage non-small cell lung cancer patients. PMID: 24645645
the initial events that likely occur when SYT-SSX2 is first expressed, and its dominant function in subverting the nuclear program of the stem cell, leading to its aberrant differentiation, as a first step toward transformation PMID: 21996728
The regulation of estrogen receptor alpha signaling by target proteins of SSX2 may explain the metastatic potential of breast cancer cells. PMID: 22344619
The cancer-related protein SSX2 interacts with the human homologue of a Ras-like GTPase interactor, RABIN3, and a novel nuclear protein, SSX2IP. PMID: 12007189
We report the identification of an HLA-DR3-restricted epitope mapping to the 37-51 region of SSX-2. PMID: 15596411
RT-PCR detection of SSX2 in paraffin-embedded tissue allowed for molecular diagnosis of synovial sarcoma. PMID: 15735574
Two kinds of cancer-testis antigen, SSX-2 and SSX-5 showed high-specific and high-frequent expression in hepatocellular carcinoma tissues, but neither of them could be detected in adjacent non-HCC tissues. PMID: 16546222

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Involvement in disease

A chromosomal aberration involving SSX2 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).

Subcellular Location

Nucleus.

Protein Families

SSX family

Tissue Specificity

Expressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and

Database Links

HGNC: 11336

OMIM: 300192

KEGG: hsa:6757

STRING: 9606.ENSP00000364665

UniGene: Hs.289105

Code	CSB-EP613674HU
Abbreviation	Recombinant Human SSX2 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Protein SSX2 (SSX2)

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