Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

WNT2

Uniprot No.

P09544

Research Area

Stem Cells

Alternative Names

Int 1 related protein; Int-1-like protein 1; Int-1-related protein; INT1L 1; INT1L1; IRP; IRP protein; ONCOGENE INT1 LIKE 1; Protein Wnt-2; Secreted growth factor; Wingless type MMTV integration site family member 2; WNT 2; WNT2; WNT2_HUMAN

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

26-360aa

Target Protein Sequence

SWWYMRATGGSSRVMCDNVPGLVSSQRQLCHRHPDVMRAISQGVAEWTAECQHQFRQHRWNCNTLDRDHSLFGRVLLRSSRESAFVYAISSAGVVFAITRACSQGEVKSCSCDPKKMGSAKDSKGIFDWGGCSDNIDYGIKFARAFVDAKERKGKDARALMNLHNNRAGRKAVKRFLKQECKCHGVSGSCTLRTCWLAMADFRKTGDYLWRKYNGAIQVVMNQDGTGFTVANERFKKPTKNDLVYFENSPDYCIRDREAGSLGTAGRVCNLTSRGMDSCEVMCCGRGYDTSHVTRMTKCGCKFHWCCAVRCQDCLEALDVHTCKAPKNADWTTAT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

39.6kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The first step of making this recombinant Human WNT2 protein is synthesizing the WNT2 gene, the synthesized gene is then cloned into an expression vector which is a cDNA plasmid that includes a promoter sequence and an antibiotic-resistant gene. It also has N-terminal 6xHis tag encoding a fusion tag for downstream protein purification or identification. The antibiotic-resistant gene enables the selection of cells carrying the plasmid in antibiotic-based media and then transfects cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The recombinant WNT2 protein is purified by ion-exchange chromatography or affinity purification. And the purity is 90%+ by SDS-PAGE.

WNT2 is an autocrine growth and differentiation factor specific for hepatic sinusoidal endothelial cells (HSECs), which act synergistically with the VEGF signaling pathway. Studies have shown that targeted disruption of the WNT2 gene causes placental defects. WNT2 has been implicated in the occurrence and progression of multiple human malignancies, including lung, breast, and colorectal cancers. WNT2 expression was found to be upregulated in human colorectal and gastric cancers, while WNT2 short interfering RNA (siRNA) or monoclonal antibodies can suppress tumor growth. WNT2 regulates beta-catenin localization in granulosa cells, and WNT2/-catenin signaling helps modulate granulosa cell proliferation.

Target Background

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons.

Gene References into Functions

Down-regulation of miR-30a-3p/5p promotes esophageal squamous cell carcinoma cell proliferation by activating the Wnt signaling pathway through inhibition of Wnt2 and Fzd2. PMID: 29259372
The data suggest that Med19 expression correlates with aggressive characteristics of bladder cancer, and Med19 knockdown suppresses the proliferation and migration of cancer cells through down-regulating the Wnt/beta-catenin pathway. PMID: 28631286
The findings of this study have shown that molecular mechanisms, including aberrant activation of the WNT2 gene signaling pathway, may be involved in the pathogenesis of preeclampsia. PMID: 29109390
SNPs in WNT2 and FOXP2 are associated with clinical symptom severity of autism spectrum disorders. PMID: 28081867
WNT2 insufficiency might cause impaired trophoblast cell proliferation and migration via downregulation of Wnt/beta-catenin signaling pathway. PMID: 28627774
Three-dimensional organotypic co-culture assays revealed that WNT2-mediated fibroblast motility and extracellular matrix remodeling enhanced cancer cell invasion of cell lines even harboring mutations in the adenomatous polyposis coli/beta-catenin pathway. PMID: 28553956
Chondrogenic potential was higher and Wnt/beta-catenin signaling was more potently activated by a GSK-3beta inhibitor in the posterior than in the anterior part of the human infant sclera. PMID: 27336854
data enforced the evidence that knockdown of c-Fos inhibited cell proliferation, migration, and invasion, and promoted the apoptosis of OS cells accompanied by altered expression of Wnt2 and Fzd9 PMID: 28665975
High WNT2 expression is associated with Cervical Carcinoma Metastasis. PMID: 27513465
targeting beta-catenin with lipofection-delivered small interfering RNA oligonucleotide inhibited the proliferation and cell cycle arrest in G0/G1 phase and increased apoptosis of fibroblast cells, accompanied by downregulation of Wnt2 and cyclin D1 as well as the phosphorylation level of glycogen synthase kinase 3 beta in the keloid fibrosis PMID: 28656880
Genetic polymorphisms in the WNT2 and CTNNB1 genes were closely associated with hepatocellular carcinoma risk and progression in a Chinese Han population PMID: 28328801
The WNT2 rs39315 G allele was associated with advanced tumor stage in hepatocellular carcinoma. PMID: 26968103
Wnt2 complements Wnt/beta-catenin signaling in regulating colorectal cancer cell proliferation. PMID: 26484565
Wnt2/beta-catenin signaling was involved in stromal-epithelial cells interaction in endometriosis. PMID: 26116230
High expression of Wnt2 is associated with pancreatic cancer progression promoted by pancreatic stellate cells. PMID: 25731618
Hypermethylation of WNT2 is associated with colorectal cancer. PMID: 23694962
The study demonistrated that expressions of Wnt-2 in high-grade brainstem gliomas were significantly higher than that in low-grade brainstem gliomas and normal brain tissues and were positively correlated with the expression of Ki-67. PMID: 23603171
Decreased placental expression of Wnt2 and increased placental expression of sFRP4 may be associated with the pathogenesis of severe preeclampsia. PMID: 23322712
We demonstrated an activation of Wnt-2 signaling via the Frizzled-8 receptor in non-small cell lung cancer cells PMID: 23815780
The skin lesions of scleroderma patients showed obviously increased expression of cytoplasmic Wnt 2, nuclear Wnt 3a and beta-catenin, but markedly lowered cell membrane expression of beta-catenin than normal skin. PMID: 23268410
significant association with SNP rs4730775 locus on chromosome 7 with genetic susceptibility to Peyronie's disease PMID: 22489561
Pdgf signaling potentiates Wnt2-Wnt7b signaling to promote high levels of Wnt activity in mesenchymal progenitors that is required for proper development of endoderm-derived organs, such as the lung PMID: 22949635
The WNT2 gene may play a suggestive role in language development in autistic disorder. PMID: 22522212
Expression of WNT2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. PMID: 22763454
Wnt/beta-catenin pathway forms a negative feedback loop during TGF-beta1 induced human normal skin fibroblast-to-myofibroblast transition PMID: 22041457
a haplotype of WNT2 (rs2896218-rs6950765: G-G) is significantly associated with ASDs. PMID: 21575668
DNA methylation within the WNT2 promoter in human placenta is associated with low birthweight. PMID: 21474991
findings provide evidence for a significant association between WNT2 and autism. PMID: 19895723
The present study does not support a major role for WNT2 in schizophrenia. PMID: 20492734
Wnt2/beta-catenin signaling pathway is constitutively activated in esophageal squamous cell carcinoma (ESCC) cells, indicating that Wnt2/beta-catenin pathway plays an essential role in carcinogenesis of the esophagus. PMID: 19857041
Wnt2 acts as an angiogenic factor for non-sinusoidal endothelium in vitro and in vivo. PMID: 19444628
Up-regulation of WNT2 mRNA by estrogen might play a key role in some cases of human breast cancer through activation of the beta-catenin - TCF signaling pathway. PMID: 11712082
Activating mutations of the WNT2 gene are not a major contributor to the development of autistic disorder. PMID: 11840514
REVIEW: role in human gastrointestinal cancer PMID: 14533014
Our interpretation of these findings is that it is unlikely that DNA variations in RELN and WNT2 play a significant role in the genetic predisposition to autism. PMID: 15048648
Target genes involved in tumor WNT pathways in response to TGF-beta1 were found in cervix cancer cells. PMID: 15878915
Wnt/beta-catenin signalling pathway is activated in most of gastric cancers, which may play pivotal roles either in gastric cancer formation or in tumour invasion and dissemination PMID: 15896469
Inhibition of Wnt2 by monoclonal antibodies is of therapeutic interest in the development of more effective treatments for malignnt pleueral mesothelioma. PMID: 15900580
The increased Wnt2 expression in gastric cancers is paralleled with reduction of membranous E-cadherin. PMID: 16132582
The WNT2 gene is upregulated along the progression from low-grade dysplasia to esophageal adenocarcioma. PMID: 16407829
Regulation of Fz4 expression by Wnt2. PMID: 17386109
inhibition of both Wnt-2 and Gal-3 had synergistic effects on suppressing canonical Wnt signaling and inducing apoptosis, suggesting that aberrant canonical Wnt/beta-catenin signaling in colorectal cancer can be regulated at multiple levels PMID: 17534895
potential coordinative effects of Wnt, NF-kappaB and/or Stat3 activation on gastric cancer formation presumably by promoting the transcription of their common target genes. PMID: 18184402
Persistent upregulation in colorectal carcinoma cases studied. PMID: 18600204
Wnt (Wnt2), Stat3, and Notch-1 and -2 signaling are correlated in human epidermal tumors. PMID: 18703315
Knockdown of Wnt2 by siRNA in human U251 glioma cells inhibited cell proliferation and invasive ability, and induced apoptotic cell death PMID: 18949017
The results suggest that WNT2 could act through its receptor FZD9 to regulate the beta-CATENIN pathway in cumulus cells, recruiting beta-CATENIN into plasma membranes and promoting the formation of adherens junctions involving CDH1. PMID: 19038973
up-regulation of the Wnt-2 protein might play a role in the development of colorectal cancers. PMID: 19239325
RNA samples from 21 neuroblastoma showed a highly significant FZD1 and/or MDR1 overexpression after treatment, underlining a role for FZD1-mediated Wnt/beta-catenin pathway in clinical chemoresistance. PMID: 19421142

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Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted.

Protein Families

Wnt family

Tissue Specificity

Expressed in brain in the thalamus, in fetal and adult lung and in placenta.

Database Links

HGNC: 12780

OMIM: 147870

KEGG: hsa:7472

STRING: 9606.ENSP00000265441

UniGene: Hs.567356

Code	CSB-YP026133HU
Abbreviation	Recombinant Human WNT2 protein
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Protein Wnt-2 (WNT2)

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