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| Code | CSB-YP026133HU |
| Size | US$1298 |
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| Description |
The first step of making this recombinant Human WNT2 protein is synthesizing the WNT2 gene, the synthesized gene is then cloned into an expression vector which is a cDNA plasmid that includes a promoter sequence and an antibiotic-resistant gene. It also has N-terminal 6xHis tag encoding a fusion tag for downstream protein purification or identification. The antibiotic-resistant gene enables the selection of cells carrying the plasmid in antibiotic-based media and then transfects cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The recombinant WNT2 protein is purified by ion-exchange chromatography or affinity purification. And the purity is 90%+ by SDS-PAGE. WNT2 is an autocrine growth and differentiation factor specific for hepatic sinusoidal endothelial cells (HSECs), which act synergistically with the VEGF signaling pathway. Studies have shown that targeted disruption of the WNT2 gene causes placental defects. WNT2 has been implicated in the occurrence and progression of multiple human malignancies, including lung, breast, and colorectal cancers. WNT2 expression was found to be upregulated in human colorectal and gastric cancers, while WNT2 short interfering RNA (siRNA) or monoclonal antibodies can suppress tumor growth. WNT2 regulates beta-catenin localization in granulosa cells, and WNT2/-catenin signaling helps modulate granulosa cell proliferation. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | WNT2 |
| Uniprot No. | P09544 |
| Research Area | Stem Cells |
| Alternative Names |
Int 1 related protein; Int-1-like protein 1; Int-1-related protein; INT1L 1; INT1L1; IRP; IRP protein; ONCOGENE INT1 LIKE 1; Protein Wnt-2; Secreted growth factor; Wingless type MMTV integration site family member 2; WNT 2; WNT2; WNT2_HUMAN
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| Species | Homo sapiens (Human) |
| Source | Yeast |
| Expression Region | 26-360aa |
| Target Protein Sequence | SWWYMRATGGSSRVMCDNVPGLVSSQRQLCHRHPDVMRAISQGVAEWTAECQHQFRQHRWNCNTLDRDHSLFGRVLLRSSRESAFVYAISSAGVVFAITRACSQGEVKSCSCDPKKMGSAKDSKGIFDWGGCSDNIDYGIKFARAFVDAKERKGKDARALMNLHNNRAGRKAVKRFLKQECKCHGVSGSCTLRTCWLAMADFRKTGDYLWRKYNGAIQVVMNQDGTGFTVANERFKKPTKNDLVYFENSPDYCIRDREAGSLGTAGRVCNLTSRGMDSCEVMCCGRGYDTSHVTRMTKCGCKFHWCCAVRCQDCLEALDVHTCKAPKNADWTTAT Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 39.6kDa |
| Protein Length | Full Length of Mature Protein |
| Tag Info |
N-terminal 6xHis-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons.
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| Gene References into Functions |
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| Subcellular Location | Secreted, extracellular space, extracellular matrix. Secreted. |
| Protein Families | Wnt family |
| Tissue Specificity | Expressed in brain in the thalamus, in fetal and adult lung and in placenta. |
| Database Links |
HGNC: 12780 OMIM: 147870 KEGG: hsa:7472 STRING: 9606.ENSP00000265441 UniGene: Hs.567356 |
