Recombinant Human Protein disulfide-isomerase A3(PDIA3)

Code CSB-EP017720HU
Size US$2466
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names PDIA3
Uniprot No. P30101
Research Area Signal Transduction
Alternative Names 58 kDa glucose regulated protein; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER 60; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER p57; ER protein 57; ER protein 60; ERp 57; ERp57; ERp60; ERp61; Glucose Regulated Protein 58 Kd; GRP 57; GRP 58; GRP57; HsT17083; p58; PDIA 3; PDIA3; PDIA3_HUMAN; Phospholipase C alpha; PI PLC; Protein disulfide isomerase A3; Protein disulfide isomerase family A member 3; Protein disulfide-isomerase A3
Species Homo sapiens (Human)
Source E.coli
Expression Region 25-505aa
Target Protein Sequence SDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 59.3 kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Gene References into Functions
  1. Data suggest that disulfide bond switch in allosteric activation/inactivation of TGM2 is example of post-translational redox regulation that is reversibly and allosterically modulated by two proteins (ERp57 and TRX). (TGM2 = transglutaminase 2; ERp57 = endoplasmic reticulum resident protein 57; TRX = thioredoxin-1) PMID: 29305423
  2. Study demonstrated that the expression of PDIA3 is frequently upregulated at the mRNA and protein levels in diffuse gliomas. Its high expression was significantly correlated with high Ki-67, more TP53 mutations and poor survival outcome. These findings imply that high expression of PDIA3 plays an important role in diffuse glioma progression. PMID: 29207176
  3. summarize available knowledge of ERp57's functions in subcellular compartments and the roles of dysregulated ERp57 in various diseases. PMID: 28723413
  4. ERp57 is involved in sperm capacitation and spermatozoa-zona pellucida binding. PMID: 28175305
  5. Thiol isomerase ERp57 is mobilized to the surface of activated platelets via a process that requires actin polymerization. PMID: 27079884
  6. Findings suggested that miR-330-5p represents a potential tumor-suppressive miRNA and plays an important role in cutaneous malignant melanoma progression by suppressing TYR and PDIA3 expression. PMID: 27363653
  7. Our study found increased expression of ERp57 in chronic hepatitis B -hepatocellular carcinoma (HBV-HCC) genesis. Such altered expression could be related to HBV infection and high ERp57 expression may lead to poor prognosis of HBV-HCC patients. PMID: 28373975
  8. The interaction of different flavonoids with PDIA3 was investigated by quenching fluorescence analysis and the effects on protein activity were evaluated. PMID: 28044092
  9. Upregulation of PDIA3 promotes ovarian cancer cell proliferation and migration. PMID: 27492604
  10. Data show that knockdown of endoplasmic reticulum resident protein 57 (ERP57) expression resulted in increase in shikonin induced apoptosis, and suggest that ERP57 might be a therapeutic option for the treatment of acute myeloid leukemia. PMID: 27415599
  11. Amyotrophic lateral sclerosis-linked PDIA3 mutations disrupt motor neuron connectivity. PMID: 26869642
  12. These data indicate that the presence of autoantibodies to PDIA3 favors the development of an efficient and specific T-cell response against PDIA3 in colorectal cancer patients. PMID: 26772958
  13. ERp57 levels are increased in the airway epithelium of asthmatic patients and in mice with allergic airways disease. PMID: 26435004
  14. LEDGF/p75 Overexpression Attenuates Oxidative Stress-Induced Necrosis and Upregulates the Oxidoreductase ERP57/PDIA3/GRP58 in Prostate Cancer PMID: 26771192
  15. it is possible that the vimentin and PDIA3 could be the candidate biomarkers specific to Achyranthes saponin therapy for rheumatoid arthritis in synovial membrane. PMID: 26724776
  16. ERp57 specifically contributes to peripheral nerve regeneration, whereas its activity is dispensable for the survival of a specific neuronal population of the central nervous system. PMID: 26361352
  17. Data suggest that regulation of PDIA3 (protein disulfide isomerase associated 3 protein) expression by microRNA-330-5p is important in maintaining hair cycle through regulation of proliferation and migration capability of keratinocytes. PMID: 26402295
  18. miR148a inhibits the proliferation and promotes paclitaxelinduced apoptosis of ovarian cancer cells, and this may be partly attributed to direct targeting of PDIA3. PMID: 26004124
  19. results suggested that ER chaperone PDIA3 plays a pivotal role in FFA-induced hepatocyte steatosis and apoptosis. PMID: 26214517
  20. The sPLA2-hPDI model constitutes a valuable tool to facilitate further insights into this process and into the (patho)physiology of sPLA2s in relation to their action intracellularly. PMID: 25763817
  21. Its cross-reaction to GFAP antibodies seems to lead to a changed signaling in the cells triggering the protective effects. PMID: 25837926
  22. PDIA3 and PDIA6 gene expression is a neoplasm aggressiveness marker in primary ductal breast cancer. PMID: 26125904
  23. study identifies ERp57 as a new modulator of PrP levels and may help with understanding the consequences of ERp57 up-regulation observed in human disease PMID: 26170458
  24. Reported increased expression of ALDH3A1, PDIA3, and PRDX2 in pterygia using a proteomic approach. These proteins are presumed to have a protective role against oxidative stress-induced apoptosis. PMID: 25221425
  25. Suggest that ERp57-STAT3 regulation functions in radioresistance of laryngeal cancer. PMID: 25605256
  26. Increased expression of PDIA3 is associated with tumor progression and the clinical behavior of gallbladder carcinoma. PMID: 23782473
  27. Results demonstrated that Grp58 appears to regulate WNT signaling by targeting beta-catenin to augment cancer invasion. PMID: 25081282
  28. Increased expression of the AGR2 and ERp57 is associated with cancer. [review] PMID: 24490732
  29. ERp57 can regulate the expression of the mitochondrial calcium uniporter (MCU) and modulate mitochondrial calcium uptake PMID: 24815697
  30. Low ERp57 expression independently predicts a poor outcome for patients with cervical cancer. PMID: 23957851
  31. ERP57 was excreted in the early stages of chronic kidney disease, and its level in urine correlated with the degree of renal fibrosis, suggesting that the secretion of ERP57 represents one of the first signs of renal fibrosis onset and progression. PMID: 23781031
  32. Tg "adduct B" was identified as primarily engaging the endoplasmic reticulum oxidoreductases ERp57 and protein disulfide isomerase PMID: 24599957
  33. Low PDIA3 expression is associated with chemoresistant colon tumors. PMID: 23255428
  34. ERp57 appears as a multifunctional chaperone that can regulate diverse biological processes to maintain the homeostasis of breast cancer cells and promote the development of bone metastasis. PMID: 23625662
  35. ERp57 positively regulates H(+),K(+)-ATPase activity apart from its chaperoning function. PMID: 24188822
  36. Platelets lacking ERp57 have defective activation of the alphaIIbbeta3 integrin and platelet aggregation. The defect in aggregation was corrected by the addition of exogenous ERp57, implicating surface ERp57 in platelet aggregation. PMID: 24030382
  37. We postulate that apoB cleavage by ER-60 within the ER lumen could facilitate proteasomal degradation of the C-terminus of translocationally-arrested apoB. PMID: 23827315
  38. Nuclear ERp57 interacts in vivo with DNA fragments in melanoma cells and is potentially involved in the transcriptional regulation of its target genes. PMID: 23587917
  39. ERp57 regulates RalA signalling by acting as a redox-sensitive guanine-nucleotide dissociation inhibitor. PMID: 23226417
  40. Data indicate that the peptide-loading complex (PLC) consists maximally of 2x tapasin-ERp57/MHC I per TAP complex, but one tapasin-ERp57/MHC I in the PLC is essential and sufficient for antigen processing. PMID: 22923333
  41. Downregulation of ER60 protease is associated with inhibition of cell proliferation in breast cancer cells in vitro. PMID: 22266712
  42. PDIA3 may play in the progression of heart failure. PMID: 22503978
  43. the vitamin D receptor (VDR) and ERp57 are critical components for actions of 1,25 dihydroxy vitamin D 3 against DNA damage, but the VDR does not require normal DNA binding or classical ligand binding to mediate photoprotection. PMID: 22322599
  44. kinetic, extent and stability of the ERp57-CRT complex, using the surface plasmon resonance spectroscopy PMID: 21996511
  45. ERp57 has roles in multiple components of platelet function and arterial thrombus formation. PMID: 22168334
  46. These results suggest that increased level of hnRNP-H and PDIA3 expression in Dengue virus infected THP1 cells assist in the viral replication by suppressing the TNF-alpha production. PMID: 22207023
  47. PDIA3 and GRP78 are IL11 targets in invasive trophoblasts PMID: 21840908
  48. Grp58 is highly expressed in patients with serious cervical stromal penetration, and further supports the theory that Grp58 is critical for cervical AD progression. PMID: 21917082
  49. ERp57 participates in signal transduction from the cell surface, in regulatory processes taking place in the nucleus, and in multimeric protein complexes involved in DNA repair. (Review) PMID: 21837552
  50. Immunostaining for both ERp57-GFP and p65 after TNF-alpha treatment indicated that nuclear translocation of these two proteins occurs independently PMID: 21598303
  51. HLA-I, TAP1, CNX, LMP7, Erp57, Tapasin and ERAP1 were down-regulated in 68%, 44%, 48%, 40%, 52%, 32% and 20% of esophageal squamous cell carcinoma lesions then, respectively. PMID: 21362330
  52. ER-60 (PDIA3) is overexpressed in a serous ovarian cancer cell line and should be investigated further as a potential biomarker of ovarian cancer. PMID: 20596667
  53. The involvement of the nuclear ERp57 complex in mechanisms associated with chromosome segregation in which specific conformational states of actin play a role in the pathway involved in paclitaxel resistance. PMID: 20596672
  54. Data suggest that further investigations are warranted to verify the involvement of ERp57 in NAFLD development. PMID: 20506389
  55. findings suggests a role for 1,25D(3)-MARRS (PDIA3)in the nucleus as a regulator of gene expression; here it may also regulate the activity of NFkappaB and other factors with which it may be interacting PMID: 20064506
  56. genes MAP3K5 and PDIA3 are associated with malignant stages of prostate cancer and therefore provide novel potential biomarkers PMID: 20035634
  57. t\The over expression of protein disulfide isomerase family A led to an gonadotropin releasing hormone receptor retention in plasma membrane. PMID: 20029959
  58. ERp57 is a necessary cofactor for the regulation of at least a subset of STAT3-dependent genes. PMID: 19995546
  59. We found that ERp57 binds to the P-domain of calreticulin, an independently folding domain comprising residues 189-288. PMID: 11842220
  60. The oxidoreductase ERp57 efficiently reduces partially folded in preference to fully folded MHC class I molecules. PMID: 12032078
  61. Stat3 and GRP58 were observed to be coassociated with cytoplasmic membranes PMID: 12060494
  62. Transglutaminase activity of human ER-60 PMID: 12162574
  63. role in class I loading process PMID: 12235131
  64. Data show that CD1d associates in the ER with both calnexin and calreticulin and with the thiol oxidoreductase ERp57 in a manner dependent on glucose trimming of its N-linked glycans. PMID: 12239218
  65. multiple domains are involved in this protein-protein interaction and that the b' domain of ERp57 cannot be replaced by that of protein disulfide isomerase PMID: 14732712
  66. characterized several fundamental structural and functional properties of ERp57, such as domain organization, shape, redox potential, and the ability to catalyze disulfide reduction, disulfide isomerization, and dithiol oxidation in substrate proteins PMID: 14871896
  67. propose a model where the region of ERp57 equivalent to the primary substrate binding site of archetypal PDI is occupied by calreticulin and suggest that the ER lectins act as adaptor molecules that define the substrate specificity of ERp57 PMID: 14871899
  68. identification of the site of interaction with calnexin and calreticulin; the contribution of both the b and b' domains to the binding with CNX and CRT was revealed PMID: 15236594
  69. ERp57 shares functional properties with protein disulfide isomerase and that both are clearly different from other oxidoreductases PMID: 15328618
  70. Considering the functional association of the two proteins, the overexpression of ERp57 observed in a variety of transformed cells might be relevant to the oncogenic properties of STAT3. PMID: 15451439
  71. 1,25D3-MARRS protein and vitamin D receptor work together to fine tune intestinal Ca2+ absorption PMID: 15862831
  72. Based on immunoblotting studies of cerebrospinal fluid (CSF) from normals, we find that the bulk of the abetas are bound to the ER chaperones, ERp57 and calreticulin. PMID: 15896298
  73. ERp57 is detected in neonatal Fc receptor (FcRn)-calnexin complexes, suggesting its role in disulfide bond formation of FcRn heavy chain. PMID: 16002696
  74. Tapasin association specifically inhibits the escape pathway required for disulfide-bond isomerization within conventional protein substrates, suggesting a specific structural role for ERp57 within the MHC class I peptide-loading complex. PMID: 16193070
  75. analysis of the role of PDI in native disulfide bond formation PMID: 16368681
  76. analyzed the cooperation of ER-60 and BiP in the oxidative refolding of denatured proteins PMID: 16428306
  77. ERp27 is bound by ERp57 both in vitro and in vivo by a similar mechanism by which ERp57 binds calreticulin PMID: 16940051
  78. ERp57 appears to cooperate with Ref-1 in the regulation of gene expression mediated by redox-sensitive transcription factors and in the adaptive response of the cell to oxidative insult. PMID: 16962936
  79. We would suggest that at least part of the mechanism of action of ERp57/GRP58 takes place through the regulation of still- unidentified stress-response genes. PMID: 17061245
  80. Recruitment of major histocompatibility complex class I molecules into the peptide-loading complex in the endoplasmic reticulum is a structural role for ERp57. PMID: 17150345
  81. ERp57 has a role in the isomerisation of non-native disulphide bonds in specific glycoprotein substrates PMID: 17170699
  82. loss of ERp57 expression correlated with more aggressive disease and could provide useful prognostic information for gastric cancer patients PMID: 17188166
  83. ERp57 is involved in differentiation of certain cancer cells and in muscle function. PMID: 17456022
  84. Results show that ERp57 is a component of human sperm acrosome proteins, which play a critical role in gamete fusion. ERp57 could be a novel phenotype marker for male infertility and has the potential to be used to assess sperm selection for IVF. PMID: 17704119
  85. Endoplasmic reticulum chaperones stabilize nicotinic receptor subunits and regulate receptor assembly. PMID: 17728248
  86. the presence of ERp57 is important for the stability of core loading complexes, and in its absence, the core loading complexes may form stable aggregates within the ER PMID: 17822402
  87. Cell stress induced by the parkinsonian mimetic, oxidopamine, is concurrent with oxidation of the chaperone, ERp57, and aggresome formation. PMID: 17848102
  88. Valsartan, a selective Ang II type 1 (AT1) receptor blocker, and N-acetylcysteine, an antioxidant, inhibited both of these modifications, indicating the contribution of AT1 receptor and reactive oxygen species to oxidation of Prx2. PMID: 17964282
  89. A ternary complex between heavy chain, ERp57, and tapasin was observed and shown to be stabilized by a disulfide between both tapasinheavy chain and tapasin-ERp57. PMID: 18039656
  90. the function of ERp57 in peptide loading is likely caused by other ERp57 functional domains or a combinatorial feature of the tapasin-ERp57 conjugate PMID: 18650385
  91. ERp57 must be physically associated with the calnexin cycle to catalyze isomerization reactions with most of its substrates PMID: 19054761
  92. the 2.6 A resolution structure of the tapasin-ERp57 core of the peptide-loading complex PMID: 19119025
  93. Knockdown of ERp57 confers cellular protection against hyperoxia- or tunicamycin-induced apoptosis by inhibition of caspase-3 activation and stimulation of BiP/GRP78 induction. PMID: 19411306
  94. tapasin conjugation with ERp57 is as critical as its integration into the membrane for efficient MHC class I assembly, surface expression, and Ag presentation to CD8+ T cells. PMID: 19701894
  95. ERp57 plays an important role in chemoresistance mechanisms in ovarian cancer by modulating the attachment of microtubules to chromosomes following paclitaxel treatment through its interaction with TUBB3. PMID: 19714814

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Subcellular Location Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome
Protein Families Protein disulfide isomerase family
Tissue Specificity Detected in the flagellum and head region of spermatozoa (at protein level).
Database Links

HGNC: 4606

OMIM: 602046

KEGG: hsa:2923

STRING: 9606.ENSP00000300289

UniGene: Hs.591095

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