Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

LIN28A

Uniprot No.

Q9H9Z2

Research Area

others

Alternative Names

Zinc finger CCHC domain-containing protein 1

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

39-112aa

Target Protein Sequence

HGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

10.2 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In the process of expressing the recombinant Human LIN28A protein, Yeast cells are transfected with a DNA expression vector that carries the gene encoding the desired protein (39-112aa). The cells are then cultured to induce the expression of the intended protein. The protein features a N-terminal 6xHis tag fusion. The recombinant Human LIN28A protein is collected and purified from the cell lysate through affinity purification, with its purity surpassing 90%, as assessed by SDS-PAGE.

Target Background

Function

RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 AND tut7 uridylyltransferaseS. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells. Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue.

Gene References into Functions

LIN28 is a very specific marker (98% specificity) for distinguishing classic gastric hepatoid carcinomas from hepatocellular carcinoma PMID: 30196987
Data indicated that LIN28A might make contribution to HSF proliferation and extracellular matrix synthesis upon thermal injury. PMID: 28771809
LIN28A inhibits lysosomeassociated membrane glycoprotein 1 protein expression in embryonic stem and bladder cancer cells. PMID: 29749495
Studied effects of lin-28 homolog A protein (Lin28A) on cells proliferation, migration, invasion and apoptosis of osteocarcinoma (OS) cells through metastasis associated lung adenocarcinoma transcript 1 (MALAT1). PMID: 29204769
Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. PMID: 29034889
LIN28A is a sensitive IHC marker for the diagnosis of Embryonal Tumor with Multilayered Rosettes , but immunoreactivity can also be seen in a proportion of Atypical Teratoid/Rhabdoid Tumors PMID: 28744687
Recent improvement in high-throughput sequencing has highlighted the potential role of LIN28/let-7 regulatory network in several developmental events. It was proposed that this pathway might represent a functional signature in cell proliferation, transition between commitment and pluripotency, and regulation of cancer and tumorigenicity. PMID: 28846452
Knockdown of miR-128a induces Lin28a expression and reverts myeloid differentiation blockage in acute myeloid leukemia. PMID: 28569789
Lin28A and Lin28B are co-expressed colon cancer tissues and have functional similarities. PMID: 27793004
this study demonstrates that Lin28A can activates androgen receptor via regulation of c-myc and promotes malignancy of ER-/Her2+ breast cancer PMID: 27494865
The results suggest that the Lin28a gene enhances the osteoblastic differentiation of human periosteum-derived cells. In addition, the Lin28a gene increases mitochondrial activity in human periosteum-derived cells. PMID: 29143345
High LIN28A expression is associated with pancreatic cancer. PMID: 26910839
the specific interaction between zinc knuckle domain of LIN28 and pre-let-7 is necessary and sufficient to induce oligouridylation. PMID: 28297670
It has been shown that the constitutive expression of Lin28a during neuronal differentiation in vitro positively and negatively affects numerous miRNAs. PMID: 27881476
ur study demonstrated that disturbance of the let-7/LIN28 double-negative feedback loop is involved in the regulation of radio- and chemo-resistance, and that let-7 and LIN28 could be employed as predictive biomarkers of response to radiotherapy or chemotherapy in non-small-cell lung cancer patients. PMID: 28235063
tight control by ESE3/EHF over the Lin28/let-7 axis is a critical barrier to malignant transformation. PMID: 27197175
MSI2 might play a crucial role in sustaining stemness and chemoresistance of liver cancer stem cells in LIN28A-dependent manner in hepatocellular carcinoma. PMID: 27721018
High LIN28A expression is associated with colorectal cancer. PMID: 27859935
LIN28A and LIN28B play cooperative roles in regulating reprogramming, naive/primed pluripotency, and stem cell metabolism. PMID: 27320042
The molecular dynamics simulations suggest that a conserved structural feature of the loop regions of pre-let-7 miRNAs is more important for LIN28 recognition than sequence conservation among members of the let-7 family or the presence of the GGAG motif in the 3' region. PMID: 28076679
Lin28A is up-regulated in 73.3 % of colon cancer patients. Lin28A-enforced expression in colon cancer cells enhanced the chemosensitivity of cancer cells to 5-FU via promoting apoptosis in a let-7 independent manner, associated with decreasing the expression of DNA damage repair protein H2AX. PMID: 26687759
Upregulation of let-7a carries the potential to reverse CCL18 induced cell proliferation and migration alteration in breast cancer cells by regulating Lin28 expression. PMID: 26898455
Data suggest that the Lin28/let-7 (lin-28 homolog protein/mirnlet7 microRNA) molecular switch plays roles in regulation of cell growth signaling pathways and in regulation of expression of metabolic enzymes. [REVIEW] PMID: 26811207
Lin28A expression promotes metabolic switching to a phenotype that relies predominantly on glycolysis as an energy source, while compromising oxidative phosphorylation. PMID: 27230676
high LIN28 expressing ovarian cancer cells secrete exosomes that can be taken up by nontumor cells and cause changes in gene expression and cell behavior associated with tumor development. IGROV1 PMID: 26583126
data document the expression profiles of the Lin28/let-7 system in rat testis along postnatal/pubertal maturation, and their perturbation in models of pubertal and hormonal manipulation PMID: 26494358
The role of Lin28 in cancer and immunity. [Review] PMID: 26945970
Erythroid-Specific Expression of LIN28A Is Sufficient for Robust Gamma-Globin Gene and Protein Expression in Adult Erythroblasts. PMID: 26675483
Our findings suggest that Lin28 plays a key role in the acquisition of resistance to AR-targeted therapies by prostate cancer cells and establish the importance of Lin28 in prostate cancer progression. PMID: 26714839
LIN28 may regulate splicing and gene expression programs that drive breast cancer subtype phenotypes. PMID: 26149387
Data implicate LIN28/RAS/MAP kinase as key drivers of tumorigenesis atypical teratoid rhabdoid tumors. PMID: 25638158
LIN28 and its regulatory microRNAs have roles in adult adrenocortical cancer PMID: 25200669
LIN28A over-expression increased HbF, reduced beta (sickle)-globin, and strongly suppressed all members of the let-7 family of miRNAs while reducing erythrocyte sickling. PMID: 25188417
Lin28A and Lin28B enhance, whereas let-7 suppresses, aerobic glycolysis via targeting pyruvate dehydrogenase kinase 1, or PDK1. PMID: 25301052
Expression of SUMO1/2/3 is dramatically enhanced by interferons through an miRNA-based mechanism involving the Lin28/let-7. PMID: 24942926
Results show the expression of Lin28 closely associated with resistance to paclitaxel in Hep3B cells which expresses high levels of Lin28. Its dysregulation inhibited let-7 family microRNA levels and upregulated the Bcl-xL, which is a target of let-7. PMID: 24970027
Incubation of a embryonal carcinoma cell line with N-methyl-mesoporphyrin IX reduces its stem cell traits. In particular it decreases OCT4, HMGA1, CCNB1, CDK4, and Lin28A protein, decreases sphere formation, and inhibits colony formation. PMID: 26045559
DFMO treatment restores balance to the LIN28/Let-7 axis and inhibits glycolytic metabolism and neurosphere formation in neuroblastoma PMID: 25415050
overexpression of Lin28 can suppress the biological behavior of gastric cancer in vitro, and let-7 miRNA may play an important role in the process PMID: 25515921
methylation modulates pluripotency in embryonic stem cells PMID: 25479749
Trim25 binds to the conserved terminal loop (CTL) of pre-let-7 and activates TuT4, allowing for more efficient Lin28a-mediated uridylation. PMID: 25457611
Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain both display LIN28A positivity. Only the latter, however, display amplification of the 19q13.42 locus involving C19MC PMID: 25748578
detected aberrant expression of LIN28A and/or LIN28B in 38% of a large series of human CRC samples (n = 595), where LIN28 expression levels were associated with invasive tumor growth PMID: 25956904
High Lin28 expression is associated with gastric cancer. PMID: 25128063
Lin28 induced EMT in breast cancer cells via downregulation of let-7a. PMID: 24349438
High LIN28 expression was an independent prognostic factor for a shorter survival in glioblastoma multiforme patients. PMID: 24475120
Results highlight a novel molecular interaction between miR-125b and Lin28 in glioblastoma stem cells. PMID: 24356103
The results have demonstrated that LIN28 is a key translational determinant of the initiation of human embryonic stem cell differentiation. PMID: 24860167
High LIN28 expression is associated with embryonal tumor with multilayered rosettes. PMID: 24311633
Data suggest that LIN28A plays functional role in regulating trophoblast differentiation/placentation; loss of LIN28A in human trophoblasts is sufficient to induce differentiation, but loss of Lin28a does not induce mouse trophoblast differentiation. PMID: 24006280

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Subcellular Location

Cytoplasm. Rough endoplasmic reticulum. Cytoplasm, P-body. Cytoplasm, Stress granule. Nucleus, nucleolus.

Protein Families

Lin-28 family

Tissue Specificity

Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors.

Database Links

HGNC: 15986

OMIM: 611043

KEGG: hsa:79727

STRING: 9606.ENSP00000254231

UniGene: Hs.86154

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Code	CSB-YP884500HU1
Abbreviation	Recombinant Human LIN28A protein, partial
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Protein lin-28 homolog A (LIN28A), partial

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