Name: Recombinant Human Protein-methionine sulfoxide oxidase MICAL2 (MICAL2), partial
Brand: CUSABIO
SKU: CSB-YP013808HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

MICAL2

Uniprot No.

O94851

Research Area

Cell Biology

Alternative Names

MICAL2; KIAA0750; MICAL2PV1; MICAL2PV2; [F-actin]-monooxygenase MICAL2; EC 1.14.13.225; Molecule interacting with CasL protein 2; MICAL-2

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

1-495aa

Target Protein Sequence

MGENEDEKQAQAGQVFENFVQASTCKGTLQAFNILTRHLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGKSCTNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDSFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPTDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDCTHYFVMTAKKQSLLDKGVIINDYIDTEMLLCAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTCMYASENAALVRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWNQGTPPLELLAERESLYRLLPQTTPENINKNFEQYTLDPGTRYPNLNSHCVRPHQVKHLYITKELEH
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

58.5kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

I am interested in CSB-YP013808HU. I need to know if you have any information about the folding in the buffer you have used to purify and if it is possible to obtain it without the tag.
Is it the protein lyophilized?

Thanks for your inquiry.
We've expressed this protein derived from Yeast, and the buffer is 20 mM Tris-HCl, 0.5M NaCl, pH 8.0，50% glycerol
We could provide the lyophilization service for free, lead time will be extended 3 business days.

Target Background

Function

Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA.

Gene References into Functions

Data suggest that MICAL2 protein might be an important regulator of epithelial to mesenchymal transition and therefore a promising target for anti-metastatic therapy. PMID: 26689989
These data show that SRF/MRTF-A signaling is regulated by MICAL-2-dependent redox regulation of nuclear actin. PMID: 24440334
although MICAL1 is auto-inhibited by its C-terminal coiled-coil region, MICAL2 remains constitutively active and affects stress fibers, suggesting differential but complementary roles for MICAL1 and MICAL2 in actin microfilament regulation PMID: 22331357
MICAL2-PV is likely to be involved in cancer progression of prostate cancer and could be a candidate as a novel molecular marker and/or target for treatment of prostate cancers. PMID: 16675569

Subcellular Location

Nucleus.

Protein Families

Mical family

Database Links

HGNC: 24693

OMIM: 608881

KEGG: hsa:9645

STRING: 9606.ENSP00000256194

UniGene: Hs.501928

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