Recombinant Human Pyruvate carboxylase, mitochondrial (PC), partial

Code CSB-YP017511HU
MSDS
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Source Yeast
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Code CSB-EP017511HU
MSDS
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Source E.coli
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Code CSB-EP017511HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017511HU
MSDS
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Source Baculovirus
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Code CSB-MP017511HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PC
Uniprot No.
Alternative Names
mitochondrial; OTTHUMP00000235155; OTTHUMP00000235156; PC; PCB; Pcx; PYC_HUMAN; Pyruvate carboxylase; Pyruvate carboxylase mitochondrial; Pyruvic carboxylase
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Gene References into Functions
  1. PC enzyme activity is much higher in the highly invasive breast cancer cell line MDA-MB-231 than in less invasive breast cancer cell lines. Strong PC suppression lowered glucose incorporation into downstream metabolites of oxaloacetate, the product of the PC reaction, including malate, citrate and aspartate. PMID: 27890529
  2. breast cancer cells proliferating as lung metastases activate PC-dependent anaplerosis in response to the lung microenvironment. PMID: 27732858
  3. Pyruvate carboxylase activates the RIG-I-like receptor-mediated antiviral immune response by targeting the MAVS signalosome. PMID: 26906558
  4. PC and PDH may be an important part of cellular adaptation to the IDH1 mutation and may serve as potential therapeutic targets PMID: 25243911
  5. These findings indicate that PC-mediated anaplerosis in early-stage NSCLC is required for tumor survival and proliferation. PMID: 25607840
  6. Increased PC expression is correlated with the malignant progression of GC, which might be a potential target for GC biotherapy. PMID: 23294723
  7. characterization of PC distal promoter PMID: 23383084
  8. PC protein is easily detectable by streptavidin blot and the presence of considerable amounts of PC in cultured human myotubes and in human muscle tissue was shown. PMID: 20807508
  9. IVS15+2-5delTAGG results in the retention of intron 15 during pre-mRNA splicing and frameshift mutations. PMID: 12112657
  10. expression and characterization of a variant by retroviral gene transfer PMID: 12437512
  11. 1 deficiency patient survived from neonatal lactic acidemia and shows an unusual subcortical leucodystrophic process. PMID: 16325442
  12. The crystal structures at 2.8-A resolution of full-length PC from Staphylococcus aureus and the C-terminal region (missing only the BC domain) of human PC, is reported. PMID: 18297087
  13. Eight novel mutations were identified in PC from 8 pyruvate carboxylase deficiency patients. PMID: 18676167
  14. The activities of pyruvate carboxylase (PC) were decreased by 65% in pancreatic islets of patients with type 2 diabetes. PMID: 19296078
  15. Nine novel mutations of the PC gene, in five unrelated patients, are reported. PMID: 19306334

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Involvement in disease
Pyruvate carboxylase deficiency (PC deficiency)
Subcellular Location
Mitochondrion matrix.
Database Links

HGNC: 8636

OMIM: 266150

KEGG: hsa:5091

STRING: 9606.ENSP00000377527

UniGene: Hs.89890

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