Recombinant Human RAD52 motif-containing protein 1 (RDM1)

Code CSB-YP839879HU
MSDS
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Source Yeast
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Code CSB-EP839879HU
MSDS
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Source E.coli
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Code CSB-EP839879HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839879HU
MSDS
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Source Baculovirus
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Code CSB-MP839879HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RDM1
Uniprot No.
Alternative Names
MGC33977; RAD52 homolog B; RAD52 motif 1; RAD52 motif containing 1; RAD52 motif-containing protein 1; RAD52B; RDM1; RDM1_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-284
Target Protein Sequence
MAELVPFAVP IESDKTLLVW ELSSGPTAEA LHHSLFTAFS QFGLLYSVRV FPNAAVAHPG FYAVIKFYSA RAAHRAQKAC DRKQLFQKSP VKVRLGTRHK AVQHQALALN SSKCQELANY YFGFNGCSKR IIKLQELSDL EERENEDSMV PLPKQSLKFF CALEVVLPSC DCRSPGIGLV EEPMDKVEEG PLSFLMKRKT AQKLAIQKAL SDAFQKLLIV VLESGKIAVE YRPSEDIVGV RCEEELHGLI QVPCSPWKQY GQEEEGYLSD FSLEEEEFRL PELD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May confer resistance to the antitumor agent cisplatin. Binds to DNA and RNA.
Gene References into Functions
  1. Due to its similarity to RAD52, we hypothesized that RDM1 potentially repairs DNA doublestrand breaks arising through DNA replication. PMID: 29845285
  2. RDM1 recognizes DNA distortions induced by cisplatin-DNA adducts in vitro; possible role in spermatogenesis PMID: 15611051
  3. The identification of 11 human cDNAs encoding RDM1 protein isoforms which is generated by alternative pre-mRNA splicing and differential usage of two translational start sites. PMID: 17905820
Subcellular Location
Nucleus. Cytoplasm. Nucleus, nucleolus. Note=Isoform 3 and isoform 10 are predominantly nuclear and nucleolar. After treatment with proteasomal inhibitors and mild heat-shock stress, isoform 1, isoform 3, isoform 5, isoform 7, isoform 8 and isoform 10 are relocalized to the nucleolus as dot-like or irregular subnuclear structures. Isoform 1 colocalized with nuclear promyelocytic leukemia (PML) and Cajal bodies (CB); this association with nuclear bodies is enhanced in response to proteotoxic stress. Isoform 3, but not isoform 1 and isoform 5, is relocalized in nucleolar caps during transcriptional arrest.; [Isoform 1]: Cytoplasm. Nucleus, PML body. Nucleus, Cajal body. Note=Isoform 1 is predominantly cytoplasmic. Isoform 1 colocalized with nuclear promyelocytic leukemia (PML) and Cajal bodies (CB); this association with nuclear bodies is enhanced in response to proteotoxic stress.
Tissue Specificity
Expressed in testis.
Database Links

HGNC: 19950

OMIM: 612896

KEGG: hsa:201299

STRING: 9606.ENSP00000293273

UniGene: Hs.194411

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