Recombinant Human Ras association domain-containing protein 5 (RASSF5)

Code CSB-EP819906HU
MSDS
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
RASSF5
Uniprot No.
Research Area
Cell Biology
Alternative Names
Maxp 1; MAXP1; MGC10823; MGC17344; New ras effector 1; NORE 1; NORE1; NORE1A; NORE1B; Novel Ras effector 1; RAP1 Binding Protein; RAPL; Ras association (RalGDS/AF 6) domain family 5; Ras association (RalGDS/AF 6) domain family member 5; Ras association domain containing family protein 5; Ras association domain-containing protein 5; Ras effector like protein; RASF5_HUMAN; RASSF 3; RASSF 5; RASSF3; RASSF5; Regulator for cell adhesion and polarization enriched in lymphoid tissue; Regulator for cell adhesion and polarization enriched in lymphoid tissues; Tumor suppressor RASSF3
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-265aa
Target Protein Sequence
MTVDSSMSSGYCSLDEELEDCFFTAKTTFFRNAQSKHLSKNVCKPVEETQRPPTLQEIKQKIDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLAGPDTEVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIQQVQKKYDKFRQKLEEALRESQGKPG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
57.4kDa
Protein Length
Full Length of Isoform 2
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis.
Gene References into Functions
  1. Ubc9 is an essential regulator of ADAP where it is required for TCR-induced membrane recruitment of the small GTPase Rap1 and its effector protein RapL. PMID: 29127148
  2. Hepatitis C virus uses NS5B to specifically suppress NORE1A, facilitating viral replication and elevated Ras signaling. PMID: 28090674
  3. Our study demonstrated that miR-214 expression was elevated and RASSF5 was down regulated in oral cancer. Moreover, miR-214 suppressed KB cell apoptosis through down regulation of RASSF5 expression PMID: 28290615
  4. mCD40L-induced cell death mediated by NORE1A expression appeared to be independent of mCD40L-induced cell death mediated by sustained JNK activation since NORE1A inhibition did not affect JNK phosphorylation and vice versa PMID: 26986513
  5. Ras induces the formation of a complex between NORE1A and the phosphatase PP1A, promoting the activation of the Rb tumor suppressor by dephosphorylation PMID: 26677227
  6. Down-regulation of RASSF5A and RASSF5C expression is a tumor-specific phenomenon. PMID: 25420558
  7. RASSF5 expression is negatively correlated with distant metastasis of osteosarcoma, and RASSF5 may function as a tumor suppressor in OS cells through activation of the MST1/LATS1 pathway. PMID: 25109282
  8. NORE1A allows Ras to qualitatively modify p53 function to promote senescence. PMID: 25778922
  9. the inactivation of RASSF5A through CpG island 1 methylation may play an important role in esophageal squamous cell carcinoma (ESCC) carcinogenesis, RASSF5A may be a functional tumor suppressor and may serve as a prognostic biomarker for ESCC. PMID: 25579665
  10. NORE1A has a role in Ras regulation of SCF(beta-TrCP) protein activity and specificity PMID: 25217643
  11. RASSF5 can act as an inhibitor or a potential positive regulator of Mst2, depending on whether it binds to Mst2 before or after activation-loop phosphorylation. PMID: 23972470
  12. Ubiquitin ligase Itch is a unique negative regulator of RASSF5. PMID: 23538446
  13. The RASSF gene family members RASSF5, RASSF6 and RASSF7 show frequent DNA methylation in neuroblastoma. PMID: 22695170
  14. crystal of NORE1 diffracted to 2.7 A resolution and belonged to space group P6(1)22, with unit-cell parameters a = b = 73.041, c = 66.092 A, alpha = beta = 90, gamma = 120 degrees PMID: 22750872
  15. These findings indicate that the control of HIPK1 stability by Mdm2-NORE1 has a major effect on cell behaviour, and epigenetic inactivation of NORE1 enables adenocarcinoma formation in vivo through HIPK1 stabilization. PMID: 22173032
  16. NORE1A has activity that suppresses the centrosome amplification induced by HU and that NORE1A mRNA down-regulation is one of the common gene abnormalities in NSCLCs, both of which imply a key preventive role of NORE1A against the carcinogenesis of NSCLC. PMID: 20434789
  17. epigenetic inactivation of NORE1 due to aberrant promoter hypermethylation is a frequent event in colorectal tumorigenesis and might be implicated in the malignant progression of colorectal tumors PMID: 20969767
  18. a novel regulatory network composed of the tumor suppressor NORE1A, the mitotic kinase Aurora A, the small GTPase Ras, and the microtubule cytoskeleton. PMID: 20339001
  19. findings define a T cell receptor "inside-out" pathway via N-SKAP1-C-RapL that regulates T cell adhesion, motility, and arrest times with dendritic cells in lymph nodes. PMID: 20346707
  20. The present investigation provided evidence that Lck-mediated phosphorylation regulates the nucleocytoplasmic shuttling and cell growth control activities of RASSF5. PMID: 20064523
  21. Nore1 is a member of a family of Ras effector/tumor suppressors that includes RASSF1 PMID: 12676952
  22. the epigenetic alteration of NORE1A is confined to lung tumors with a wild-type K-ras: 88% (15 of 17) of the tumors with NORE1 hypermethylation did not harbor a K-ras mutation (P=0.008, Fisher's exact test) PMID: 15378027
  23. Rap1 activation contributes to directional vascular endothelial cell migration accompanied by extension of microtubules on which RAPL localizes PMID: 15569673
  24. results indicate that RASSF1A epigenetic changes are an early event in thyroid tumor pathogenesis and progression PMID: 15980887
  25. Suppression of NORE1A, a known Ras effector, in PAX8-PPARgamma fusion-carrying follicular thyroid cancer. PMID: 16352687
  26. association of NORE1A with cytoskeletal elements is essential for NORE1A-induced growth suppression and that the ERK pathway is a target for NORE1A growth-suppressive activities PMID: 16421102
  27. The candidate tumor suppressor gene NORE1B is epigenetically down-regulated in hepatocellular carcinoma. PMID: 16516329
  28. These findings suggest that endogenous Nore1B recruits active Ras to the APC-T cell interface and mediates the interaction between Ras and Carma1. PMID: 16520020
  29. The NORE1A gene was never found epigenetically methylated in hepatitic or non-hepatitic liver. PMID: 16606445
  30. Nuclear localization of RASSF5 is critical for its cell growth control activity. PMID: 17320110
  31. interaction surfaces in RAPL-Rap1 and RAPL-Rap2 complexes are different and that a single residue in the switch I region of Rap proteins (residue 39) contributes considerably to the different kinetics of these protein-protein interactions. PMID: 17716979
  32. nuclear export of NORE1A via nuclear export signal is involved in the NORE1A-mediated induction of apoptosis PMID: 18211824
  33. NORE1A promoter methylation is a rare event in neuroblastoma cells and primary tumors. Other mechanisms are likely to account for the frequent reduction of NORE1A mRNA expression. Also, antitumorigenic role of NORE1A in human neuroblastoma is evident. PMID: 18452173
  34. Study describes the crystal structure of Ras in complex with the Ras binding domain (RBD) of NORE1A; the contact area of NORE1A is extended as compared with other Ras effectors & provides a rationale for an exceptionally long lifetime of the complex. PMID: 18596699
  35. findings showed that only 4% of the glioma tumors revealed a methylated promoter for NORE1A PMID: 18616639
  36. Data show that degradation by calpains is a novel mechanism for downregulation of NORE1A and RASSF1A proteins and might be the mechanism allowing cancer cells to escape growth suppression. PMID: 19098985
  37. NORE1B suppresses replication and transformation of cells as effectively as RASSF1A and is a putative tumor suppressor gene. NORE1B interacts with RASSF1A and loss of one of these may lead to uncontrolled growth and transformation of hepatocytes. PMID: 19118008
  38. NORE1A activates p21(CIP1) via promoting p53 nuclear localization. PMID: 19435914

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Note=Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
Tissue Specificity
Widely expressed. Frequently down-regulated in lung tumor cell lines and primary lung tumors.
Database Links

HGNC: 17609

OMIM: 607020

KEGG: hsa:83593

STRING: 9606.ENSP00000347443

UniGene: Hs.497579

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