Recombinant Human Ras-related protein Rab-34 (RAB34)

Code CSB-YP880148HU
MSDS
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Source Yeast
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Code CSB-EP880148HU
MSDS
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Source E.coli
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Code CSB-EP880148HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880148HU
MSDS
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Source Baculovirus
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Code CSB-MP880148HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAB34
Uniprot No.
Alternative Names
Rab-39; RAB34; RAB34, member RAS oncogene family; RAB34_HUMAN; RAB39; RAH; Ras-related protein Rab-34; Ras-related protein Rab-39; Ras-related protein Rah
Species
Homo sapiens (Human)
Expression Region
1-259
Target Protein Sequence
MNILAPVRRD RVLAELPQCL RKEAALHGHK DFHPRVTCAC QEHRTGTVGF KISKVIVVGD LSVGKTCLIN RFCKDTFDKN YKATIGVDFE MERFEVLGIP FSLQLWDTAG QERFKCIAST YYRGAQAIII VFNLNDVASL EHTKQWLADA LKENDPSSVL LFLVGSKKDL STPAQYALME KDALQVAQEM KAEYWAVSSL TGENVREFFF RVAALTFEAN VLAELEKSGA RRIGDVVRIN SDDSNLYLTA SKKKPTCCP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transport protein involved in the redistribution of lysosomes to the peri-Golgi region. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Acts also as a positive regulator of hedgehog signaling and regulates ciliary function.
Gene References into Functions
  1. The present study demonstrated that RAB34 plays an important role in the initiation and progression of hepatocellular carcinoma (HCC). Our results suggest a new therapeutic target for the clinical treatment of HCC. PMID: 29048615
  2. Rab34 activity is modulated by SopD2 and is required for maturation of the Salmonella containing vacuole. PMID: 28185347
  3. NARR is ubiquitously expressed and resides in nucleoli where it colocalizes with ribosomal DNA gene clusters. PMID: 21586586
  4. MMP19 were positively correlated with RAB34. PMID: 25501506
  5. Although Rab34 regulates both lysosomal positioning and lysosome to phagosome fusion, the latter effect is not due to the regulation of the cytoplasmic accessibility of lysosomes to phagosomes by Rab34. PMID: 23871933
  6. Rab36 may regulate the spatial distribution of late endosomes and lysosomes through a similar mechanism to Rab34. PMID: 19961360
  7. Data suggest that diacylglycerol-activated hmunc13 serves as an effector of Rab34, mediating lysosome-Golgi trafficking. PMID: 16138900
  8. define Rab34 as a novel member of the secretory pathway acting at the Golgi PMID: 17881736
  9. B coxsackievirus entry depends on occludin and require the activity of Rab34, Ras, and Rab5, GTPases known to regulate macropinocytosis. PMID: 18005733
  10. mechanistic basis for high glucose-induced protein secretion is through interaction of munc13 and rab34, indicating a potentially critical role for this newly described pathway in the pathogenesis of DN. PMID: 19641095

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Subcellular Location
Cytoplasm. Golgi apparatus. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Cell projection, cilium.
Protein Families
Small GTPase superfamily, Rab family
Database Links

HGNC: 16519

OMIM: 610917

KEGG: hsa:83871

STRING: 9606.ENSP00000410403

UniGene: Hs.301853

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