Recombinant Human Receptor-type tyrosine-protein phosphatase S (PTPRS), partial

Code CSB-YP613393HU
MSDS
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Source Yeast
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Code CSB-EP613393HU
MSDS
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Source E.coli
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Code CSB-EP613393HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP613393HU
MSDS
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Source Baculovirus
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Code CSB-MP613393HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PTPRS
Uniprot No.
Alternative Names
Protein tyrosine phosphatase PTPsigma ; Protein tyrosine phosphatase receptor type S; Protein tyrosine phosphatase receptor type sigma; Ptprs; PTPRS_HUMAN; PTPSIGMA ; R PTP sigma; R-PTP-S; R-PTP-sigma; Receptor-type tyrosine-protein phosphatase S; Receptor-type tyrosine-protein phosphatase sigma
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycan. Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth. Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection. Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2. Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb. Functions as tyrosine phosphatase. Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3. Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases. Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta.
Gene References into Functions
  1. Study identified several CpG sites and specifically several CpGs in the PTPRS and PER3 genes differentially methylated between obese and non-obese children, suggesting a role for DNA methylation concerning development of childhood obesity. PMID: 28614626
  2. interaction of receptor type of protein tyrosine phosphatase sigma (RPTPsigma) with a glycosaminoglycan library PMID: 29420785
  3. Receptor protein tyrosine phosphatase sigma (RPTPsigma) regulates neuronal extension and acts as a presynaptic nexus for multiple protein and proteoglycan interactions during synaptogenesis. PMID: 25385546
  4. Data demonstrate that synoviocytes are regulated by an RPTPsigma-dependent proteoglycan switch in vivo, which can be targeted for rheumatoid arthritis therapy. PMID: 25995222
  5. represents an evolutionarily conserved pDC-specific inhibitory receptor, and is required to prevent spontaneous IFN production and immune-mediated intestinal inflammation PMID: 26231120
  6. crystallographic analyses of a shared HSPG-CSPG binding site reveal a conformational plasticity that can accommodate diverse glycosaminoglycans with comparable affinities; HS and analogs induced RPTPsigma ectodomain oligomerization which was inhibited by CS PMID: 21454754
  7. Structural insights into the homology and differences between mouse protein tyrosine phosphatase-sigma and human protein tyrosine phosphatase-sigma. PMID: 22027896
  8. Intragenic microdeletions of the EGFR phosphatase PTPRS were found frequently (26%) in head and neck cancers, identifying this gene as a target of 19p13 loss. PMID: 22065749
  9. Transgenic zebrafish carrying a promoter-driven expression vector reveal the increased density of olfactory sensory neuron-specific cell synapses. PMID: 21812780
  10. Data show that PTPsigma localizes to PtdIns3P-positive membranes in cells, and this vesicular localization is enhanced during autophagy. PMID: 21303930
  11. The receptor protein tyrosine phosphatase sigma (RPTPsigma) has now been identified as a receptor for inhibitory chondroitin sulfate proteoglycans. PMID: 20179269
  12. Transient overexpression of the short splice variant 3 of RPTPR Sigma conferred alpha-LTX induced secretion to hamster insulinoma (HIT-15) cells. In contrast, the long splice variant 2 was inactive in secretion or in a single cell assay. PMID: 16552719
  13. Three SNPs that flank exon 8 are associated with ulcerative colitis. The presence of these SNPs is associated with novel splicing that removes the third immunoglobulin-like domain (exon 9) from the extracellular portion of PTPsigma. PMID: 17614280
  14. SNPs rs1143699, rs4807015, and rs1978237 confer an increased risk of developing type 2 diabetes mellitus. PMID: 17893260
  15. In colorectal tumors, microsatellite repeats mutation rates are higher than the mean mutation frequency PMID: 19000305

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Perikaryon. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. Cell junction, synapse, postsynaptic density. Cell projection, neuron projection. Cell projection, growth cone.
Protein Families
Protein-tyrosine phosphatase family, Receptor class 2A subfamily
Tissue Specificity
Detected in peripheral blood plasmacytoid dendritic cells (at protein level). Detected in all tissues tested except for placenta and liver. Detected in peripheral blood plasmacytoid dendritic cells.
Database Links

HGNC: 9681

OMIM: 601576

KEGG: hsa:5802

STRING: 9606.ENSP00000349932

UniGene: Hs.744928

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