Recombinant Human Rho guanine nucleotide exchange factor 16 (ARHGEF16)

Code CSB-YP730133HU
MSDS
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Source Yeast
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Code CSB-EP730133HU
MSDS
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Source E.coli
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Code CSB-EP730133HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730133HU
MSDS
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Source Baculovirus
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Code CSB-MP730133HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARHGEF16
Uniprot No.
Alternative Names
ARHGEF 16; ARHGEF16; ARHGG_HUMAN; Ephexin 4; Ephexin-4; EPHEXIN4; GEF16; NBR; Putative Neuroblastoma Protein; Rho guanine exchange factor (GEF) 16; Rho guanine nucleotide exchange factor (GEF) 16; Rho guanine nucleotide exchange factor 16
Species
Homo sapiens (Human)
Expression Region
2-709
Target Protein Sequence
AQRHSDSSL EEKLLGHRFH SELRLDAGGN PASGLPMVRG SPRVRDDAAF QPQVPAPPQP RPPGHEEPWP IVLSTESPAA LKLGTQQLIP KSLAVASKAK TPARHQSFGA AVLSREAARR DPKLLPAPSF SLDDMDVDKD PGGMLRRNLR NQSYRAAMKG LGKPGGQGDA IQLSPKLQAL AEEPSQPHTR SPAKNKKTLG RKRGHKGSFK DDPQLYQEIQ ERGLNTSQES DDDILDESSS PEGTQKVDAT IVVKSYRPAQ VTWSQLPEVV ELGILDQLST EERKRQEAMF EILTSEFSYQ HSLSILVEEF LQSKELRATV TQMEHHHLFS NILDVLGASQ RFFEDLEQRH KAQVLVEDIS DILEEHAEKH FHPYIAYCSN EVYQQRTLQK LISSNAAFRE ALREIERRPA CGGLPMLSFL ILPMQRVTRL PLLMDTLCLK TQGHSERYKA ASRALKAISK LVRQCNEGAH RMERMEQMYT LHTQLDFSKV KSLPLISASR WLLKRGELFL VEETGLFRKI ASRPTCYLFL FNDVLVVTKK KSEESYMVQD YAQMNHIQVE KIEPSELPLP GGGNRSSSVP HPFQVTLLRN SEGRQEQLLL SSDSASDRAR WIVALTHSER QWQGLSSKGD LPQVEITKAF FAKQADEVTL QQADVVLVLQ QEDGWLYGER LRDGETGWFP EDFARFITSR VAVEGNVRRM ERLRVETDV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
Gene References into Functions
  1. Sixty-four novel structural analogues of Y27632 were synthesized and tested for their ability to persistently inhibit the transformation of NIH3T3 cells by Rho guanidine exchange factor 16 (ARHGEF16) or Ras. PMID: 19707205
  2. These data suggest that HPV16 E6, Tip-1 and ARHGEF16 may cooperate to activate Cdc42 and support a potential link between the expression of HPV16 E6 and Cdc42 activation PMID: 21139582
  3. The ephexin4 protein mediates cell migration through a RhoG-dependent mechanism. PMID: 20679435
Subcellular Location
Cytoplasm.
Database Links

HGNC: 15515

KEGG: hsa:27237

STRING: 9606.ENSP00000367629

UniGene: Hs.87435

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