Recombinant Human Rho guanine nucleotide exchange factor 3 (ARHGEF3)

Code CSB-YP878863HU
MSDS
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Source Yeast
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Code CSB-EP878863HU
MSDS
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Source E.coli
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Code CSB-EP878863HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878863HU
MSDS
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Source Baculovirus
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Code CSB-MP878863HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARHGEF3
Uniprot No.
Alternative Names
ARHGEF3; Rho guanine nucleotide exchange factor 3; Exchange factor found in platelets and leukemic and neuronal tissues; XPLN
Species
Homo sapiens (Human)
Expression Region
1-526
Target Protein Sequence
MVAKDYPFYL TVKRANCSLE LPPASGPAKD AEEPSNKRVK PLSRVTSLAN LIPPVKATPL KRFSQTLQRS ISFRSESRPD ILAPRPWSRN AAPSSTKRRD SKLWSETFDV CVNQMLTSKE IKRQEAIFEL SQGEEDLIED LKLAKKAYHD PMLKLSIMTE QELNQIFGTL DSLIPLHEEL LSQLRDVRKP DGSTEHVGPI LVGWLPCLSS YDSYCSNQVA AKALLDHKKQ DHRVQDFLQR CLESPFSRKL DLWNFLDIPR SRLVKYPLLL REILRHTPND NPDQQHLEEA INIIQGIVAE INTKTGESEC RYYKERLLYL EEGQKDSLID SSRVLCCHGE LKNNRGVKLH VFLFQEVLVI TRAVTHNEQL CYQLYRQPIP VKDLLLEDLQ DGEVRLGGSL RGAFSNNERI KNFFRVSFKN GSQSQTHSLQ ANDTFNKQQW LNCIRQAKET VLCAAGQAGV LDSEGSFLNP TTGSRELQGE TKLEQMDQSD SESDCSMDTS EVSLDCERME QTDSSCGNSR HGESNV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.
Gene References into Functions
  1. This is the first study describing the involvement of XPLN in the regulation of the mTORC2-SPARC axis, and these findings may help uncover regulatory mechanisms mediated by XPLN in idiopathic pulmonary fibrosis PMID: 28315487
  2. Our findings suggest that increased expression of ARHGEF3 plays a critical oncogenic role in nasopharyngeal carcinoma pathogenesis PMID: 27028992
  3. Data suggest that modulation of ARHGEF3 gene expression in humans with a promoter-localized SNP plays a role in human megakaryocytes and human platelet function-a finding resulting from the biological follow-up of human genetic studies. Arhgef3 KO mice partially recapitulate the human phenotype. PMID: 28542600
  4. ARHGEF3 has a crucial role in myeloid differentiation. PMID: 25494542
  5. ARHGEF3 and RHOA are potential regulators of a number of genes in bone cells, with influences on ACTA2 evident in both osteoblast-like and osteoclast-like cells. PMID: 24840563
  6. XPLN is an endogenous inhibitor of mTORC2. PMID: 24043828
  7. the association between rs7646054 in ARHGEF3 and bone mineral density observed in Caucasians was not replicated in the chinese samples. PMID: 19727905
  8. a guanine nucleotide exchange factor for RhoA and RhoB, but not RhoC PMID: 12221096
  9. genetic variation in ARHGEF3 plays a role in the determination of bone density in Caucasian women. This data implicates the RhoGTPase-RhoGEF pathway in osteoporosis. PMID: 18499081

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Subcellular Location
Cytoplasm.
Tissue Specificity
Widely expressed. Highest levels are found in adult brain and skeletal muscle. Lower levels are found in heart and kidney.
Database Links

HGNC: 683

OMIM: 612115

KEGG: hsa:50650

STRING: 9606.ENSP00000341071

UniGene: Hs.476402

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