Recombinant Human Rho-related GTP-binding protein RhoU (RHOU)

Code CSB-YP745331HU
MSDS
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Source Yeast
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Code CSB-EP745331HU
MSDS
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Source E.coli
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Code CSB-EP745331HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP745331HU
MSDS
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Source Baculovirus
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Code CSB-MP745331HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RHOU
Uniprot No.
Alternative Names
ARHU; CDC42 L1; CDC42 like GTPase; CDC42-like GTPase 1; CDC42L1; G28K; GTP binding protein like 1; GTP binding protein SB128; GTP-binding protein-like 1; hG28K; mG28K; Ras homolog gene family member U; Ras homolog gene family member U isoform CRA a; Ras like gene family member U; Rho GTPase like protein ARHU; Rho GTPase-like protein ARHU; Rho related GTP binding protein RhoU; Rho-related GTP-binding protein RhoU; Rhou; RHOU_HUMAN; Ryu GTPase; Wnt-1 responsive Cdc42 homolog 1; Wnt1 responsive Cdc42 homolog; WRCH 1; WRCH-1
Species
Homo sapiens (Human)
Expression Region
1-258
Target Protein Sequence
MPPQQGDPAF PDRCEAPPVP PRRERGGRGG RGPGEPGGRG RAGGAEGRGV KCVLVGDGAV GKTSLVVSYT TNGYPTEYIP TAFDNFSAVV SVDGRPVRLQ LCDTAGQDEF DKLRPLCYTN TDIFLLCFSV VSPSSFQNVS EKWVPEIRCH CPKAPIILVG TQSDLREDVK VLIELDKCKE KPVPEEAAKL CAEEIKAASY IECSALTQKN LKEVFDAAIV AGIQYSDTQQ QPKKSKSRTP DKMKNLSKSW WKKYCCFV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.
Gene References into Functions
  1. These results unravel a role for RhoU not only in regulating the migratory features of malignant plasma cells, but also in controlling cell cycle progression. PMID: 29440639
  2. This review addresses the developmental roles of 2 GTPases of the Rho family, RhoV/Chp and RhoU/Wrch. PMID: 26555387
  3. PAK4 and RhoU cooperate to drive adhesion turnover and promote cell migration. PMID: 26598620
  4. Data indicate that upregulated miR-126 upon coxsackievirus B3 (CVB3) infection targets SPRED1, LRP6, and WRCH1 genes, mediating cross-talk between ERK1/2 and Wnt/beta-catenin pathways, and thus promoting viral replication. PMID: 23811937
  5. multiple upstream signals may converge on Wrch1 directly through its SH3 domain-binding properties PMID: 23183748
  6. results indicate that NOTCH1 mutation stimulates T-cell acute lymphoblastic leukaemia cell migration through RhoU upregulation that could contribute to the leukaemia cell dissemination PMID: 22349824
  7. data suggest a unique regulatory mechanism by which RhoU interaction with SH3 adaptor proteins might serve to integrate growth factor receptor signaling with RhoU activatio PMID: 21508312
  8. A screen for Wrch-1 binding-partners identified the novel RhoGAP protein, ARHGAP30, as a Wrch-1 interactor. PMID: 21565175
  9. Studies revealed a requirement for serum-stimulated tyrosine phosphorylation of Wrch1 at residue Y254 within its C-terminal membrane targeting domain, mediated by the nonreceptor tyrosine kinase Src. PMID: 20547754
  10. Down regulation of WRCH1 by estradiol in breast cancer and altered expression of WRCH1 is associated with other cancers PMID: 11894124
  11. G28K cDNA encodes a protein of 258 amino acids with homology to the cell division cycle 42/G25K protein; this gene is located at 1q42.11-q42.3; G28K was abundantly expressed in the stomach and the small intestine PMID: 14731133
  12. These data suggest that Wrch-1 regulates cell migration by multiple mechanisms: on the one hand Wrch-1 controls focal adhesions by regulating myosin light chain and on the other hand Wrch-1 stimulates the activation of Akt and JNK. PMID: 17504809
  13. Wrch-1-induced morphological and growth transformation may occur in part through Par6-mediated disruption of tight junction and actin organization. PMID: 19064640
  14. As an essential actor of osteoclast differentiation, the atypical RhoGTPase Wrch1/RhoU could be an interesting target for the development of novel antiresorptive drugs. PMID: 19135548

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Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Cell junction, focal adhesion. Cell projection, podosome.
Protein Families
Small GTPase superfamily, Rho family
Tissue Specificity
Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.
Database Links

HGNC: 17794

OMIM: 606366

KEGG: hsa:58480

STRING: 9606.ENSP00000355652

UniGene: Hs.647774

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