Recombinant Human Ribonuclease H2 subunit B (RNASEH2B)

Code CSB-YP732934HU
MSDS
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Source Yeast
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Code CSB-EP732934HU
MSDS
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Source E.coli
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Code CSB-EP732934HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP732934HU
MSDS
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Source Baculovirus
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Code CSB-MP732934HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RNASEH2B
Uniprot No.
Alternative Names
RNASEH2B; DLEU8; Ribonuclease H2 subunit B; RNase H2 subunit B; Aicardi-Goutieres syndrome 2 protein; AGS2; Deleted in lymphocytic leukemia 8; Ribonuclease HI subunit B
Species
Homo sapiens (Human)
Expression Region
2-312
Target Protein Sequence
AAGVDCGDG VGARQHVFLV SEYLKDASKK MKNGLMFVKL VNPCSGEGAI YLFNMCLQQL FEVKVFKEKH HSWFINQSVQ SGGLLHFATP VDPLFLLLHY LIKADKEGKF QPLDQVVVDN VFPNCILLLK LPGLEKLLHH VTEEKGNPEI DNKKYYKYSK EKTLKWLEKK VNQTVAALKT NNVNVSSRVQ STAFFSGDQA STDKEEDYIR YAHGLISDYI PKELSDDLSK YLKLPEPSAS LPNPPSKKIK LSDEPVEAKE DYTKFNTKDL KTEKKNSKMT AAQKALAKVD KSGMKSIDTF FGVKNKKKIG KV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
Gene References into Functions
  1. Results implicate rare variants in the Aicardi-Goutieres syndrome genes ADAR and RNASEH2B and a type I interferon signature in glioma and prostate carcinoma risk and tumorigenesis, consistent with a genetic basis underlying inflammation-driven malignant transformation in glioma and prostate carcinoma development. PMID: 29030706
  2. This study reviewed that Neurologic Phenotypes Associated with Mutations in RNASEH2B in patients with Aicardi-Goutieres Syndrome. PMID: 27643693
  3. Aicardi-Goutieres syndrome 2 is caused by mutations in the ribonuclease H2 subunit B gene RNASEH2B. PMID: 25906927
  4. RNase H2 complex is assembled in the cytosol and imported into the nucleus in an RNase H2B-dependent manner. PMID: 24986920
  5. A genome-wide search for homozygosity in Aicardi-Goutieres syndrome patients in the Faroe Islands revealed one single 15.6 Mb region of homozygosity on chromosome 13, which included RNASEH2B, where a splice site mutation c.322-3C>G was identified. PMID: 22882256
  6. This study demonistrated that ribonuclease H2 mutation releated to Aicardi-Goutieres syndrome. PMID: 21862834
  7. Congenital infection is seen with preserved neurological function, most frequently due to RNASEH2B mutations. PMID: 18422679
  8. Examination of the effect of several Aicardi-Goutieres Syndrome-related mutations in the B subunit of RNase H2. PMID: 19015152
  9. patients with genetic deficiency develop the spontaneous inflammatory myocarditis PMID: 19120481

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Involvement in disease
Aicardi-Goutieres syndrome 2 (AGS2)
Subcellular Location
Nucleus.
Protein Families
RNase H2 subunit B family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 25671

OMIM: 610181

KEGG: hsa:79621

STRING: 9606.ENSP00000337623

UniGene: Hs.306291

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