Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
AdoMet synthase 2;Methionine adenosyltransferase 2;MAT 2;Methionine adenosyltransferase II;MAT-II
Species
Homo sapiens (Human)
Expression Region
1-395aa
Target Protein Sequence
MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-395 form the expressed segment for recombinant Human MAT2A. The expected molecular weight for the MAT2A protein is calculated to be 49.3 kDa. This protein is generated in a baculovirus-based system. The MAT2A gene fragment has been modified by fusing the C-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant MAT2A protein during the following stages.
Human S-adenosylmethionine synthase isoform type-2 (MAT2A) is a crucial enzyme involved in cellular methylation processes. MAT2A catalyzes the formation of S-adenosylmethionine (SAM), a universal methyl donor critical for various cellular reactions, including DNA methylation, histone modification, and neurotransmitter synthesis. MAT2A is predominantly expressed in the liver and regulates the balance between SAM and S-adenosylhomocysteine (SAH), influencing cellular methylation potential. Dysregulation of MAT2A is implicated in liver diseases and cancer, emphasizing its role in maintaining epigenetic and metabolic homeostasis. Research on MAT2A continues to uncover its intricate involvement in cellular processes, making it a potential target for therapeutic interventions in diseases related to aberrant methylation patterns.