Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

MAT2A

Uniprot No.

P31153

Research Area

Metabolism

Alternative Names

AdoMet synthase 2;Methionine adenosyltransferase 2;MAT 2;Methionine adenosyltransferase II;MAT-II

Species

Homo sapiens (Human)

Source

Baculovirus

Expression Region

1-395aa

Target Protein Sequence

MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

49.3 kDa

Protein Length

Full Length

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-395 form the expressed segment for recombinant Human MAT2A. The expected molecular weight for the MAT2A protein is calculated to be 49.3 kDa. This protein is generated in a baculovirus-based system. The MAT2A gene fragment has been modified by fusing the C-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant MAT2A protein during the following stages.

Human S-adenosylmethionine synthase isoform type-2 (MAT2A) is a crucial enzyme involved in cellular methylation processes. MAT2A catalyzes the formation of S-adenosylmethionine (SAM), a universal methyl donor critical for various cellular reactions, including DNA methylation, histone modification, and neurotransmitter synthesis. MAT2A is predominantly expressed in the liver and regulates the balance between SAM and S-adenosylhomocysteine (SAH), influencing cellular methylation potential. Dysregulation of MAT2A is implicated in liver diseases and cancer, emphasizing its role in maintaining epigenetic and metabolic homeostasis. Research on MAT2A continues to uncover its intricate involvement in cellular processes, making it a potential target for therapeutic interventions in diseases related to aberrant methylation patterns.

Target Background

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Gene References into Functions

MAT2A expression is upregulated through mRNA stabilization upon SAM depletion. PMID: 29262316
Increased MAT2A expression is associated with recurrence in hepatocellular carcinoma. PMID: 29448301
High MAT2A expression is associated with tamoxifen-resistant breast cancer. PMID: 26418898
METTL16 that controls MAT2A intron retention in response to intracellular SAM levels; splicing of the MAT2A retained intron is rapidly induced upon Met depletion, and this effect requires a conserved hairpin which is a METTL16 m6A substrate. PMID: 28525753
methionine adenosyltransferase II alpha (MAT2A), and the arginine methyltransferase, PRMT5, as vulnerable enzymes in cells with MTAP deletion. PMID: 27068473
crystal structures of human MATalpha2 containing various bound ligands. PMID: 26858410
acetylation decreased in hepatocellular cancer PMID: 25925782
Mutagenesis of MATalpha2 and MATbeta phospho-sites destabilized them and prevented hepatic stellate cell trans-differentiation. PMID: 25294683
The data presented here support the conclusion that rare genetic variants in MAT2A predispose individuals to thoracic aortic disease. PMID: 25557781
Results suggest that MAT2A is downregulated in cancer tissues of renal cell carcinomas patients and has function of tumor suppressor though repressing the expression of heme oxygenase-1. PMID: 24636201
Differential gene expression of BACE1 and PSEN1 in the knockdown cells, which is possibly a consequence of MAT2A deregulation and may indicate a self regulatory mechanism. PMID: 22879628
hypoxia-induced MAT2A expression is HIF-1alpha dependent PMID: 21460102
Insulin-like growth factor 1 (IGF1) activates methionine adenosyltransferase 2A MAT2A) transcription by both known and novel pathways in colon cancer cells. PMID: 21406062
X protein of hepatitis B virus inhibits apoptosis in hepatoma cells through enhancing the methionine adenosyltransferase 2A gene expression and reducing S-adenosylmethionine production PMID: 21247894
regulation of expression in hepatocarcinoma cells by L-methionine availability PMID: 12660248
beta subunit associates with cirrhosis and cancer providing a proliferative advantage in hepatoma cells through its interaction with methionine adenosyltransferase II alpha2 and down-regulation of S-adenosylmethionine levels. PMID: 12671891
up-regulation of MAT2A also provides a growth advantage and s-adenosylmethionine and methylthioadenosine can block mitogenic signaling in colon cancer cells PMID: 17631143
Lower expression of both MAT2A and MAT2beta and interfere with leptin signaling in liver cancer cells. PMID: 18041713
This work was carried out to examine the role of MAT II activity and S-adenosylmethionine biosynthesis in the survival of leukemic T cells. PMID: 19048023

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Protein Families

AdoMet synthase family

Tissue Specificity

Detected in kidney.

Database Links

HGNC: 6904

OMIM: 601468

KEGG: hsa:4144

STRING: 9606.ENSP00000303147

UniGene: Hs.516157

Code	CSB-BP013517HU
Abbreviation	Recombinant Human MAT2A protein
MSDS	MSDS Datasheet
Size	$400
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human S-adenosylmethionine synthase isoform type-2 (MAT2A)

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