Recombinant Human Serpin H1 (SERPINH1)

In Stock
Code CSB-EP021087HUb0
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Target Names
SERPINH1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
(47 kDa heat shock protein)(Arsenic-transactivated protein 3)(AsTP3)(Cell proliferation-inducing gene 14 protein)(Collagen-binding protein)(Colligin)(Rheumatoid arthritis-related antigen RA-A47)
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
19-418aa
Target Protein Sequence
AEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
50.6 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Gene References into Functions
  1. Our present data suggest that HSP47 is an important prognostic factor and an attractive therapeutic target in laryngeal squamous cell carcinoma (LSCC)due to its influence on the biological behaviour of LSCC cells PMID: 28849239
  2. an endoplasmic reticulum complex of resident chaperones that includes HSP47, FKBP65, and BiP regulating the activity of LH2. PMID: 28177155
  3. These observations indicate that this system is appropriate for detecting the interaction between HSP47 and collagen, and could be applied to high-throughput screening for drugs capable of suppressing and/or curing fibrosis. PMID: 29438711
  4. miR-29b can reduce collagen biosynthesis during skin wound healing likely via post-transcriptional inhibition of HSP47 expression. PMID: 27477081
  5. HSP47 expression in patients with colorectal cancer and the number of HSP47-positive spindle cells in the tumor stroma were significantly higher compared with those in adjacent normal colonic mucosa, and the number of the latter cells increased with tumor progression. PMID: 27925182
  6. The essential parts of the Golgi stress response from the perspective of the organelle autoregulation. The pathways of the mammalian Golgi stress response have been identified, specifically the HSP47 pathway. PMID: 28179603
  7. Overexpression of LOXL2 and SERPINH1 was observed in clinical specimens of lung cancer and fibrotic lesions. Downregulation of miR-29a caused overexpression of LOXL2 and SERPINH1 in lung cancer and IPF, suggesting that these genes are involved in the pathogenesis of these two diseases. PMID: 27488440
  8. The changes in the SERPINH1 and SERPINF1 genes in patients with osteogenesis imperfect were synonymous polymorphisms or missense changes located in non-coding regions. PMID: 27706701
  9. Overexpression of HSP47 is associated with poor prognosis in patients with esophageal squamous cell carcinoma and this is consistent with the function of HSP47 in terms of increased cell proliferation and colony formation. PMID: 25953518
  10. A novel homozygous variant in SERPINH1 associated with a severe, lethal presentation of osteogenesis imperfecta with hydranencephaly. PMID: 27677223
  11. The present study demonstrates that HSP47 promotes glioma angiogenesis and highlights the importance of HSP47 as an attractive therapeutic target of GBM. PMID: 25758142
  12. Although the chemical chaperone 4-PBA partially restores the solubility of the Hsp47 OI mutants, collagen-binding activity of Hsp47 was not improved. PMID: 26692483
  13. Data show that the expression of heat shock protein 47 (HSP47) was increased in the peripheral blood mononuclear cells and plasma from scleroderma patients. PMID: 26091621
  14. Mutations in the HSP47 and FKBP65 produce a moderately severe form of Osteogenesis imperfect. PMID: 25510505
  15. In patients with schistosomiasis japonica, TGF-beta1 participates not only in the inflammatory process, but also in the fibrotic process in which Hsp47 and CTGF probably play a key role. PMID: 25111595
  16. Hsp47 expression promotes cancer progression in part by enhancing deposition of extracellular matrix proteins. PMID: 25744716
  17. IL-17A-induced HSP47 expression is involved in collagen I expression in intestinal subepithelial myofibroblasts, which might contribute to intestinal fibrosis in Crohn's disease. PMID: 24534724
  18. silencing of the HSP47 gene significantly inhibited cell migration and invasion in cancer cells and the expression of HSP47 was upregulated in cancer tissues and cervical intraepithelial neoplasia, as demonstrated by immunostaining. PMID: 24141696
  19. miR-29b down-regulates HSP47 and LOX expression. PMID: 24650661
  20. HSP47 is a novel glioma-associated antigen PMID: 24623841
  21. TRAIL induced HSF1 inactivation leads to the suppression of Hsp47-dependent collagen production in activated human hepatic stellate cells. PMID: 23587601
  22. Correlative Hsp47 expression in fibroblasts with bFGF in inflammatory cells may contribute to stromal fibrosis and obstruction in colorectal carcinoma PMID: 23265436
  23. NMR and mutational identification of the collagen-binding site of the chaperone Hsp47 PMID: 23049894
  24. Hsp47 may be related to the TGF-beta1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts. PMID: 22967132
  25. Hsp47 recognizes the triple-helix form of procollagen in vitro and in vivo. PMID: 22235129
  26. Overexpression of HSP47 decreased the secretion of heterotrimers containing the mutant collagen alpha5(IV) chain. PMID: 21187648
  27. HSP47 and fascin expression may play role in the pathogenesis of invasive ductal carcinoma of the breast and prostatic adenocarcinoma because their expression is significantly higher than their normal counterpart. PMID: 20701077
  28. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis PMID: 19067716
  29. down-regulated KLF4, CHGA, GPX3, SST and LIPF, together with up-regulated SERPINH1, THY1 and INHBA is an 8-gene signature for gastric cancer PMID: 20043075
  30. Increased heat shock protein 47 expression is associated with esophageal squamous cell carcinoma. PMID: 20112500
  31. The enhancement of HSP47 expression by TGF-beta and IL-1 beta has been confirmed in embryonic lung fibroblasts. PMID: 11994473
  32. induced in cicatricial pemphigoid: possible role(s) in dermal fibrosis PMID: 12061838
  33. Results indicate a novel means by which type I collagen production is regulated by the endoplasmic reticulum constituent, Hsp47. PMID: 12163502
  34. Modulates the production of the endostatin precursor collagen XVIII in head and neck carcinomas PMID: 12174873
  35. levels of HSP47 protein and autoantibodies to HSP47 in the sera of patients with rheumatic autoimmune diseases PMID: 12659832
  36. gene expression profiling in epidermolysis bullosa acquisita PMID: 12824005
  37. high levels of expression of Hsp47 and adult and oncofetal fibronectin in Dupuytren's contracture suggests that cell-mediated alterations in the extracellular environment may play an important role in the disease process PMID: 15047128
  38. HSP47 has a role in aging and photoaging in human fibroblasts PMID: 15247019
  39. The surface-exposed RA-A47 may induce autoantibodies and inflammatory reactions in autoimmune disease situations such as rheumatoid arthritis. PMID: 15389525
  40. HSP47 is constitutively expressed in human hepatic stellate cells and may be a target for antifibrotic therapy. PMID: 15806139
  41. Our results suggest the existence of different fibrotic pathways among these groups involved in the expression of HSP47 and type I procollagen. PMID: 15955241
  42. results suggest nitric oxide has dual effects on collagen synthesis by fibroblasts: the direct stimulation of collagen synthesis due to the up-regulation of procollagen alphaI(1) mRNA, and an indirect effect through the increase of HSP47 mRNA expression PMID: 16171977
  43. analysis of the client recognition mechanism of HSP47 PMID: 16326708
  44. analysis of recognition of the collagen triple helix by chaperone HSP47 PMID: 16484215
  45. A functional SNP in the promoter of the SERPINH1 gene increases risk of preterm premature rupture of membranes in African Americans. PMID: 16938879
  46. The anti-fibrotic effect of pirfenidone may be mediated through direct inhibition of collagen type I expression and inhibition of HSP47 expression in lung fibroblasts. PMID: 18093617
  47. HSP47-positive fibroblasts were main constituent cell of dermatofibroma. PMID: 18095990
  48. These studies define a new haplotype in the SERPINH1 gene that modifies risk of an adverse obstetrical outcome. PMID: 18205191
  49. During keratin preparation from cultured human tumor cell lines, Hsps might be associated with keratin expression in tumor cells PMID: 18293509
  50. Hsp47 was exposed on surface of GPVI-activated platelets; inhibition of Hsp47 abolished platelet aggregation in response to collagen, but partially reduced aggregation in response to other agonists; propose Hsp47 may play a role in hemostasis & thrombosis PMID: 19341245

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Involvement in disease
Osteogenesis imperfecta 10 (OI10)
Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
Serpin family
Database Links

HGNC: 1546

OMIM: 600943

KEGG: hsa:871

STRING: 9606.ENSP00000350894

UniGene: Hs.596449

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