Recombinant Human Slit homolog 3 protein (SLIT3), partial

Code CSB-YP021769HU
MSDS
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Source Yeast
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Code CSB-EP021769HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021769HU
MSDS
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Source Baculovirus
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Code CSB-MP021769HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLIT3
Uniprot No.
Alternative Names
FLJ10764; KIAA0814; MEGF 5; MEGF5; Multiple EGF-like domains protein 5; Multiple epidermal growth factor like domains 5 ; Multiple epidermal growth factor-like domains protein 5; SLIL 2; SLIL2 ; SLIT 1; Slit 2; Slit 3; Slit homolog 3 (Drosophila); Slit homolog 3; Slit homolog 3 protein; Slit-3; SLIT1; Slit2; Slit3; SLIT3_HUMAN
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
Gene References into Functions
  1. Overexpression of Slit3 induces its tumor suppressive effects in Breast cancer. PMID: 26542734
  2. Results show that silencing of Slit3 promoted proliferation, migration and invasion of A549 cells and induced epithelial-mesenchymal transition suggesting that Slit3 functions as a tumor suppressor in lung carcinoma. PMID: 26045181
  3. Estrogen-dependent expression of SLIT3 may play a key role in regulating nerve-vessel interactions within the complex microenvironment of endometriosis lesions. PMID: 25051436
  4. SLIT3 is increased with labour, and both amnion and myometrial studies describe a pro-inflammatory effect of SLIT3 in these tissues. PMID: 24286238
  5. High SLIT3 expression is associated with high-grade gliomas. PMID: 24158112
  6. SLIT3-ROBO4 activation promotes vascular network formation in human engineered tissue and angiogenesis in vivo. PMID: 24090675
  7. results implicate the involvement of miR-218-2 and its host gene SLIT3 in thyroid cancer cell invasion, migration, and proliferation PMID: 23720784
  8. Slit3 treatment increased the in vivo homing efficiency of CD34 HSPCs to the BM in NOD/SCID mice, whereas Slit3-exposed HSPC migration in vitro was inhibited. Results support a role for Slit3 in human HSPC migration in vitro and homing in vivo. PMID: 22614124
  9. SLIT3 duplication on 5q35.1 predisposes to major depressive disorder PMID: 21152026
  10. The chemorepellent Slit3 promotes monocyte migration. PMID: 21078908
  11. Slit3 inhibits Robo3-induced invasion of synovial fibroblasts in rheumatoid arthritis. PMID: 20298552
  12. Many variants in SLIT3 tend to associate with schizophrenia susceptibility in the Chinese Han population. PMID: 15231749
  13. data suggest that Slit proteins play a key role in guiding dorsally projecting cranial motoneurons and in facilitating their neural tube exit PMID: 16162649
  14. In fetal and embryonic stem cell cultures Slit-3 inhibited neurite outgrowth. PMID: 16840550
  15. functions to promote angiogenesis in coordinating organogenesis during embryonic development PMID: 19741192

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Subcellular Location
Secreted.
Tissue Specificity
Predominantly expressed in thyroid.
Database Links

HGNC: 11087

OMIM: 603745

KEGG: hsa:6586

STRING: 9606.ENSP00000430333

UniGene: Hs.552087

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