Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
(Na(+)(-)(Natrium iodide transporter)(Sodium-iodide symporter)(Na(+)(-)(Solute carrier family 5 member 5)
Species
Homo sapiens (Human)
Expression Region
547-643aa
Target Protein Sequence
SCLTGPTKRSTLAPGLLWWDLARQTASVAPKEEVAILDDNLVKGPEELPTGNKKPPGFLPTNEDRLFFLGQKELEGAGSWTPCVGHDGGRDQQETNL
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Human SLC5A5 covers amino acids 547-643. The calculated molecular weight for this SLC5A5 protein is 17.4 kDa. This SLC5A5 protein is produced using e.coli expression system. The SLC5A5 coding gene included the C-terminal 6xHis tag, which simplifies the detection and purification processes of the recombinant SLC5A5 protein in following stages of expression and purification.
The human sodium/iodide cotransporter (SLC5A5) is a crucial membrane protein involved in the active transport of iodide ions across the cell membrane. Mainly expressed in thyroid follicular cells, SLC5A5 plays a pivotal role in thyroid hormone synthesis. It facilitates the uptake of iodide from the bloodstream into thyroid cells, an essential step for the production of thyroid hormones, thyroxine (T4), and triiodothyronine (T3). This process is integral to the regulation of metabolism and overall physiological homeostasis. Additionally, due to its ability to concentrate iodide, SLC5A5 has been utilized in medical imaging and radioiodine therapy for thyroid disorders, including thyroid cancer.