Recombinant Human Solute carrier family 22 member 16 (SLC22A16), partial

Code CSB-YP771451HU
MSDS
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Source Yeast
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Code CSB-EP771451HU
MSDS
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Source E.coli
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Code CSB-EP771451HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP771451HU
MSDS
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Source Baculovirus
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Code CSB-MP771451HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLC22A16
Uniprot No.
Alternative Names
SLC22A16; OCT6; Solute carrier family 22 member 16; Carnitine transporter 2; CT2; Fly-like putative transporter 2; FLIPT2; Flipt 2; Organic cation transporter OKB1; Organic cation/carnitine transporter 6
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
High affinity carnitine transporter; the uptake is partially sodium-ion dependent. Thought to mediate the L-carnitine secretion mechanism from testis epididymal epithelium into the lumen which is involved in the maturation of spermatozoa. Also transports organic cations such as tetraethylammonium (TEA) and doxorubicin. The uptake of TEA is inhibited by various organic cations. The uptake of doxorubicin is sodium-independent.
Gene References into Functions
  1. Allele C of ABCB1 and allele A of SLC22A16 tended to have a greater association with grade 3/4 febrile neutropenia. PMID: 28036387
  2. Deletions in SLC22A16, a doxorubicin transporter, are associated with a failure to achieve event free survival in immunochemotherapy-treated diffuse large B-cell lymphoma. PMID: 26314988
  3. findings may greatly contribute to the elucidation of the roles of the Oct and Myc proteins in osteoblast direct reprogramming. The results may also lead to establishment of novel regenerative therapy for various bone resorption diseases PMID: 26499074
  4. Knockdown of CT2 reduced the growth and viability of acute myeloid leukemia cells with high expression of CT2 (OCI-AML2 and HL60), but not low expression. CT2 knockdown reduced basal oxygen consumption without a concomitant increase in glycolysis. PMID: 25998464
  5. Both gene and protein expression of OCT6 were decreased in both CDDP-resistant lung cancer cell lines compared with their expression in their respective parental cells. PMID: 25772757
  6. cytotoxic assays, stable transfectants of leukemic Jurkat cells overexpressing SLC22A16 cells became significantly more sensitive to doxorubicin PMID: 15963465
  7. Variant alleles in the ABCB1, SLC22A16 and CYP2B6 genes are associated with response to AC therapy in the treatment of breast cancer. PMID: 20179710
  8. that hCT2 can mediate bleomycin-A5 and polyamine uptake, and that the rate of bleomycin-A5 accumulation may account for the differential response to the drug in patients. PMID: 20037140
  9. identification, expression and function of human CT2 as a carnitine transporter in testis mediating transport of l-carnitine PMID: 12089149
  10. Identification as a novel organic cation transporter preferentially expressed in hematopoietic cells and leukemias PMID: 12384147
  11. may be possible to use progestins to increase the response of endometrioid endometrial carcinoma with SLC22A16 expression to adriamycin-based chemotherapeutic regimens PMID: 17197897
  12. Exploratory study suggests that the c.146A>G variation could contribute to variations in the pharmacokinetics of doxorubicin and doxorubicinol in Asian breast cancer patients. PMID: 17559346
  13. SLC22A16 was abundantly expressed in clear-cell adenocarcinomaof the ovary. PMID: 17581421

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Subcellular Location
Membrane; Multi-pass membrane protein. Cell membrane. Note=Detected in the plasma membrane of Sertoli cells and in the luminal membrane of epithelial cells in the epididymis.
Protein Families
Major facilitator (TC 2.A.1) superfamily, Organic cation transporter (TC 2.A.1.19) family
Tissue Specificity
Widely expressed at low levels in adult tissues and fetal brain, lung, liver and kidney. Expressed in testis and epididymis (at protein level). Expressed at lower levels in bone marrow and fetal liver. Expressed in hematopoietic cells, including CD34(+) l
Database Links

HGNC: 20302

OMIM: 608276

KEGG: hsa:85413

STRING: 9606.ENSP00000357915

UniGene: Hs.520319

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